在自然繁育条件下,牦牛一生中仅有1%的卵泡发育成熟并排卵,其余绝大部分卵泡则在发育不同阶段走向了闭锁退化。在一个发情周期中,异常的卵泡闭锁导致无法受孕,是造成牦牛繁殖效率低下的原因之一。Yes相关蛋白1 (Yes-associated protein ...
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在自然繁育条件下,牦牛一生中仅有1%的卵泡发育成熟并排卵,其余绝大部分卵泡则在发育不同阶段走向了闭锁退化。在一个发情周期中,异常的卵泡闭锁导致无法受孕,是造成牦牛繁殖效率低下的原因之一。Yes相关蛋白1 (Yes-associated protein 1,YAP-1)作为一个非DNA结合的转录共激活子和多功能的细胞内连接蛋白,促进或抑制细胞的增殖和凋亡,调控动物有机体多个器官大小和组织再生。本研究旨在探讨牦牛繁殖活动的分子调控机制,阐明YAP1基因在卵泡发育中的生物学作用,为提高牦牛繁殖效率提供基础资料。
MicroRNAs are small non-coding endogenous RNA molecules that down-regulate the expression of target genes in a sequence-dependent *** studies indicate that miRNAs are mechanistically involved in mammalian corpus luteu...
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MicroRNAs are small non-coding endogenous RNA molecules that down-regulate the expression of target genes in a sequence-dependent *** studies indicate that miRNAs are mechanistically involved in mammalian corpus luteum(CL).However,few studies have profiled the different miRNA expression patterns in the bovine CL and follicular *** this study,a heterologous microarray was employed to investigate the different miRNA expression *** the 34 differentially expressed miRNAs between CL and follicular ovaries,20 miRNAs were preferentially expressed in CL ovaries,while 14 miRNAs were richer in the follicular *** 89 orthologous miRNAs were also *** these 89 miRNAs,76 were up-regulated more than 1.5 times in CL ovaries,and 13 in follicular ***-time RT-PCR assay was used to validate the microarray *** genes and transcription factor binding motifs of some differentially expressed miRNAs were predicted to uncover the mechanisms involved in bovine luteal function *** results detail the data on bovine ovary and are useful for biological functions investigation of miRNAs in bovine CL.
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