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检索条件"任意字段=中国畜牧兽医学会动物繁殖学分会第十八届学术研讨会暨中日韩第四届动物繁殖学术交流会"

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CHARACTERIZATION OF PORCINE OCT4 UPSTREAM REGULATORY REGION STRUCTURE AND FUNCTION

CHARACTERIZATION OF PORCINE OCT4 UPSTREAM REGULATORY REGION ...

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中国畜牧兽医学会动物繁殖学分会第十八届学术研讨会暨中日韩第四届动物繁殖学术交流会

作者： Seung-Hun Kim Dongchan Son Kwang-Hwan Choi Dong-Kyung Lee Jong-Nam Oh Ji Young Oh Tae-Yeong Park Chang-Kyu Lee Department of Agricultural Biotechnology Animal Biotechnology Majorand Research Institute for Agriculture and Life ScienceSeoul National University Institute of Green Bio Science and Technology Seoul National University

The transcription factor OCT4 has a critical role in central regulator of maintaining pluripotency and ***,it is an important marker of undifferentiating status in early mammalian embryonic development and embryonic stem *** regulatory regions including core promoter,distal and proximal enhancers of OCT4 have been investigated in various species and they have four conserved

关键词： CHARACTERIZATION OF PORCINE OCT4 UPSTREAM REGULATORY REGION STRUCTURE AND FUNCTION

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OVEREXPRESSION OF KDM4D LEADS TO LOSS OF DNA METHYLATION AT ICRS OF XIST AND OCT4IN GOAT FIBROBLAST CELLS

OVEREXPRESSION OF KDM4D LEADS TO LOSS OF DNA METHYLATION AT ...

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中国畜牧兽医学会动物繁殖学分会第十八届学术研讨会暨中日韩第四届动物繁殖学术交流会

作者： Mingtian Deng Yongjie wan Guomin Zhang Rihong Guo Wenjun Zhou Zifei Liu Feng wang Jiangsu Livestock Embryo Engineering Laboratory College of Animal Science and TechnologyNanjing Agricultural University Jiangsu Engineering Technology Research Center of Meat Sheep & Goat Industry Nanjing Agricultural University

Imprinted genes play vital development roles and are generally organized in clusters controlled by DNA methylation at *** studies demonstrated that maintenance of H3K9 at ICRs is key to the regulation of imprinted ***... 详细信息

关键词： KDM OVEREXPRESSION OF KDM4D LEADS TO LOSS OF DNA METHYLATION AT ICRS OF XIST AND OCT4IN GOAT FIBROBLAST CELLS DNA

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PCI-24781 CAN IMPROVE IN VITRO AND IN VIVO DEVELOPMENTAL CAPACITY OF PIG SOMATIC CELL NUCLEAR TRANSFER EMBRYOS

PCI-24781 CAN IMPROVE IN VITRO AND IN VIVO DEVELOPMENTAL CAP...

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中国畜牧兽医学会动物繁殖学分会第十八届学术研讨会暨中日韩第四届动物繁殖学术交流会

作者：康锦丹 Hai-Ying Zhu Long Jin Qing Guo Yu-chen Zhang Xiao-Chen Li Xiao-Xu Xing Mei-Fu Xuan Guang-Lei Zhang Qi-Rong Luo Zheng-Yun Cui Cheng-Du Cui 尹熙俊 Yanbian University Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering

Introduction Previous studies suggest that histone deacetylase inhibitors(HDACi)improved the development of porcine somatic cell nuclear transfer(SCNT)*** aim of this study was to examine the effects of PCI-24781,a novel broad-spectrum phenyl hydroxamic acid HDACi,on the in vitro development of pig SCNT *** and methods Matured eggs were cultured in medium supplemented with 0.05 M sucrose and 0.4μg/mL

关键词： PCI-24781 CAN IMPROVE IN VITRO AND IN VIVO DEVELOPMENTAL CAPACITY OF PIG SOMATIC CELL NUCLEAR TRANSFER EMBRYOS HDACi

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TAUROURSODEOXYCHOLIC ACID ENHANCES PRE-IMPLANTATION EMBRYOS DEVELOPMENT AFTER ICSI OF EVAPORATIVELY DRIED BOAR SPERMATOZOA

TAUROURSODEOXYCHOLIC ACID ENHANCES PRE-IMPLANTATION EMBRYOS ...

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中国畜牧兽医学会动物繁殖学分会第十八届学术研讨会暨中日韩第四届动物繁殖学术交流会

作者： Xiao Xia Li Yun Fei Diao Shi yong Wang Xin Yan Cao Hai Jun Wei Yong gang Zhao Yi Feng Yang Dan Li Li Bao Zeng Xu Min Kyu Kim State Key Laboratory for Molecular Biology of Special Economic Animals Institute of Special Animal and Plant SciencesChinese Academy of agricultural Sciences Department of Animal Science and Biotechnology College of Agriculture and Life ScienceChungnam National University

Evaporative-drying,which is alternative cryopreservation of gametes,does not requir liquid nitrogen(LN2)and freeze-dryer machine during sample preparation;however,the results were not successful as *** is an important determinant of the normal development of pre-implantation embryos in *** acid(TUDCA)as an endogenous bile acid has been reported to

关键词： TUDCA TAUROURSODEOXYCHOLIC ACID ENHANCES PRE-IMPLANTATION EMBRYOS DEVELOPMENT AFTER ICSI OF EVAPORATIVELY DRIED BOAR SPERMATOZOA

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PROGESTERONE INFLUENCES CYTOPLASMIC MATURATION IN PORCINE OOCYTES DEVELOPING IN VITRO

PROGESTERONE INFLUENCES CYTOPLASMIC MATURATION IN PORCINE OO...

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中国畜牧兽医学会动物繁殖学分会第十八届学术研讨会暨中日韩第四届动物繁殖学术交流会

作者： Bao Yuan Shuang Liang Jiabao Zhang Nam-Hyung Kim Department of Laboratory Animal Science College of Animal SciencesJilin University Molecular Embryology Laboratory Department of Animal SciencesChungbuk National University

Progesterone(P4),an ovarian steroid hormone,is a key regulator of female *** in vitro maturation progesterone is produced by cumulus cells,and its level in the medium was increased by stimulation with *** effects of P4 on oocyte and embryo development have been assessed by several investigations,however,with contradictory *** this study,we explored the effect of progesterone on porcine oocyte meiotic maturation,both nuclear and cytoplasmic *** showed that compared with control groups,presence of P4 during oocyte maturation did not inhibit the polar body extrusions,but significantly increased intracellular reactive oxygen species(ROS) and glutathione *** incidence of parthenogentically activated embryos that developed to the blastocyst stage was higher(p < 0.05) when oocytes were exposed to P4 as those compared to *** cell number was higher and apoptosis was lower in the P4 treated ***,the P4 specific inhibitor Mifepristone(RU486)blocked porcine oocyte maturation,presented as reducing the percentages of oocytes that underwent germinal vesicle breakdown(GVBD) and first polar body ***486 affected MPF and MAPK activity and the polyadenylation status of maternal mRNAs by weakening and shortening signal ***,these data suggest that P4 influences the meiotic and cytoplasmic maturation of porcine *** least,partially by decreasing their polyadenylation,altering the expression of other maternal genes.

关键词： Progesterone Mifepristone Pig oocyte In vitro maturation(IVM) Embryonic development

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REPRODUCTIVE AXIS GENE REGULATION OF PHOTOSTIMULATION AND PHOTOREFRACTORINESS OF YANGZHOU GOOSE GANDERS

REPRODUCTIVE AXIS GENE REGULATION OF PHOTOSTIMULATION AND PH...

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中国畜牧兽医学会动物繁殖学分会第十八届学术研讨会暨中日韩第四届动物繁殖学术交流会

作者： Huanxi Zhu Xibing Shao Zhe Chen Zhendan Shi Laboratory of Animal Improvement and Reproduction Institute of Animal ScienceJiangsu Academy of Agricultural Sciences

Yangzhou goose is a long day breeder that has been increasingly produced in eastern China *** lighting programs are required for controlling the breeding *** mechanisms regulating the reproductive axis of during the light induced stimulation and refractoriness is poorly understood,but which is important for optimization of photoperiodic programs and reproductive *** study therefore was carried out to investigate the regulatory mechanisms by artificial photoperiodic programs on reproductive activities and genes expression patterns of reproductive axis in Yangzhou goose ganders.A flock of Yangzhou goose replacement fowls aged 6 months were divided into 2 groups of 300 *** flocks were initially exposed to a short daily photoperiod of 8 h(8L:16D) for either 2(Group A) or 3(Group B) months,and then both were switched,respectively,to a longer 12 h daily photoperiod(12L:12D),for a duration of 8 *** samples were collected every 2 weeks through wing veins,anti-clotted,and plasma separated and stored at-20℃ till assay for *** the course of experiment,6 to 8ganders in each group were randomly caught and slaughtered on days 123 ages,177 ages,200 ages,243 ages and 317 ages of days,and then testes were collected,and weighed.A piece of the left testis sample was fixed in 4%formaldehyde solution for histological *** the same time,hypothalamus,pituitary gland tissue samples were collected,and,together with part of testis sample,snap frozen in liquid nitrogen,for the purposes of gene expression *** results are showed as follows:Exposure to 8 h short photoperiod inhibited testes *** daily photoperiod from 8 h to 12 h rapidly stimulated testis expansion,and increased plasma testosterone concentrations,in both groups of ganders,which both reached peak levels at 2 months after switching long 12 h daily *** stimulation of reproductive activities was associated

关键词： Yangzhou goose ganders Reproductive activities Photoperiod Gene mRNA expressions

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EFFECTS OF MICROBUBBLED WATER SUPPLY ON TOTAL EGG PRODUCTION RATE,EGG WEIGHTS,AND BODY WEIGHT IN EARLY LAYING HENS

EFFECTS OF MICROBUBBLED WATER SUPPLY ON TOTAL EGG PRODUCTION...

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中国畜牧兽医学会动物繁殖学分会第十八届学术研讨会暨中日韩第四届动物繁殖学术交流会

作者： Dong-Mok Lee Byoung Boo Seo Biomedical Manufacturing Engineering Center Korea Institute of Industrial Technology(KITECH) Department of Animal Resources Daegu University Institute of Life and Environment Daegu University

Generally,microbubbles are defined as bubbles have micrometer-diameter;the diameter of these bubbles is ranging from 10 micrometer to several hundred *** are characterized with slow rise speed to water surface and low friction *** studies have done by national and international research teams with regards to applications to medical purposes,environmental water purification,aquaculture,industrial purposes,home and ***,in the current study,we investigated if microbubbled water supply impacts on total egg production rate,egg weights,and body weight gain in early laying *** hens(i.e.,ISA brown) were divided into either control group or intervention group(16 per each group);the control group was supplied with general tab water while the intervention group was supplied with microbubbled water twice a day(morning and afternoon).The total egg production rate was compared between the control and intervention group up to 30 weeks;as results,after 23-25 weeks,the intervention group showed higher total egg production rate compared to those of the control group(10%).After this,it was not significantly different compared to the control group(p >0.05).And,the weight of eggs was not also significantly different between the groups(56.2 g and 5.96 g for the control and intervention group,respectively;p > 0.05).When it comes to the early body weight gain of laying hens,it was slightly higher in the intervention group(approximately 5%) yet no statistical significant was noted(p >0.05) demonstrating that microbubbles may have no influence on egg *** together,these results herein support that microbubbled water supply may improve the total egg production rate in early laying *** investigations are required if microbubbled water supply differently influence on the total egg production rate,egg weights,and body weight gain in late laying hens.

关键词： Microbubbles ISA Brown Egg rate Egg weight Body weight

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CONCENTRATION OF AMH AND MRNA EXPRESSION OF FOUR TGF-B FAMILY GENES IN RELATION TO DIFFERENT FOLLICULAR DIAMETER IN BUFFALO

CONCENTRATION OF AMH AND MRNA EXPRESSION OF FOUR TGF-B FAMIL...

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中国畜牧兽医学会动物繁殖学分会第十八届学术研讨会暨中日韩第四届动物繁殖学术交流会

作者： Yaping Wang Zan Li Feng Xu Tingxian Deng Chunying Pang Xianwei Liang Bianca Gasparrini Guo Huahua Liguo Yang Aixin Liang Key Laboratory of Agricultural Animal Genetics Breeding and Reproduction of Ministry of EducationCollege of Animal Science and TechnologyHuazhong Agricultural University Guangxi Key Laboratory of Buffalo Genetics Reproduction and BreedingNanning Department of Veterinary Medicine and Animal Production Federico Ⅱ University

Objectives:Anti-Mullerian hormone(AMH) produced by granulosa cells(GCs) of growing follicles,is a reliable endocrine marker for antral follicle count and ovarian reserve,and could be used a potential predictive marker of embryo ***,little study is reported in buffalo species with low *** of the study was to determine the intrafollicular concentration of AMH correlation and mRNA expression of AMH,bone morphogenetic protein-2(BMP-2),bone morphogenetic protein-6(BMP-6) and inhibin-A(INHA) genes which belong to TGF-p family,and to investigate their possible *** and Methods:All visible antral follicles were aspirated from abattoir-derived ovaries collected from 22 synchronized *** cells and follicular fluid(FF) were collected from small(3-5 mm,n=68),medium(5-8 mm,n=12) and large(>8 mm,n=8) follicles,*** relative mRNA expressions of AMH,BMP-2,BMP-6 and INHA genes were detected using Real-time PCR,and the intrafollicular concentration of AMH was measured by ELISA and RIA *** and Pearson correlation analyses were used in this ***:The concentrations of AMH sharply decreased from 239.65 ± 20.47 ng/mL in small follicles to 68.94±11.21 ng/ml in medium follicles,followed by a further reduction to 28.19±7.69 ng/ml in large ***,mRNA expression of AMH gene was higher in small follicles rather than in medium and large follicles(p<0.05),and a significant association between concentration and mRNA of AMH was found(r=0.538,p<0.01).In contrast,mRNA expression of INHA showed an increasing trend with growing follicular diameter,with large follicles having higher levels than small follicles(p<0.01).However,no significant differences were found among small,medium and large follicles in the mRNA expression of both BMP-2 and BMP-6 ***:The expression and secretion levels of AMH showed decreasing change with the increasing follicular size,which exhibited reverse trend to th

关键词： AMH TGF-β Granulosa cells Buffalo

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