Vaccinia virus(VV) provided prophylactic immunity to variola virus,however,s ignificant adverse health effects have been reported following vaccine administration. In recent years,VV has also been successfully used as...
详细信息
Vaccinia virus(VV) provided prophylactic immunity to variola virus,however,s ignificant adverse health effects have been reported following vaccine administration. In recent years,VV has also been successfully used as a live vaccine vector for i nfectious *** is therefore necessary to ensure the safty of vaccinia-based vac cine vectors. Vaccinia virus Tian Tan(VTT) is a member of the orthopoxvirus genus,whic h is the most relevant to *** was also reported that TI4L gene was correlate d to *** approach adopted in this study involved gene integration at a sit e of TI4L locus using the well-characterized Cre-loxP site-specific gene recombinatio n system,which has been used widely in animal cells and analysis of gene function. Cre recombinase catalyzes site-specific and irreversible cleavage of DNA segments flanked by unique 34 bp loxP target *** conditional gene-targeting techn
暂无评论