Progesterone(P4),an ovarian steroid hormone,is a key regulator of female *** in vitro maturation progesterone is produced by cumulus cells,and its level in the medium was increased by stimulation with *** effects of P...
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Progesterone(P4),an ovarian steroid hormone,is a key regulator of female *** in vitro maturation progesterone is produced by cumulus cells,and its level in the medium was increased by stimulation with *** effects of P4 on oocyte and embryo development have been assessed by several investigations,however,with contradictory *** this study,we explored the effect of progesterone on porcine oocyte meiotic maturation,both nuclear and cytoplasmic *** showed that compared with control groups,presence of P4 during oocyte maturation did not inhibit the polar body extrusions,but significantly increased intracellular reactive oxygen species(ROS) and glutathione *** incidence of parthenogentically activated embryos that developed to the blastocyst stage was higher(p < 0.05) when oocytes were exposed to P4 as those compared to *** cell number was higher and apoptosis was lower in the P4 treated ***,the P4 specific inhibitor Mifepristone(RU486)blocked porcine oocyte maturation,presented as reducing the percentages of oocytes that underwent germinal vesicle breakdown(GVBD) and first polar body ***486 affected MPF and MAPK activity and the polyadenylation status of maternal mRNAs by weakening and shortening signal ***,these data suggest that P4 influences the meiotic and cytoplasmic maturation of porcine *** least,partially by decreasing their polyadenylation,altering the expression of other maternal genes.
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