为了研究转化生长因子β3(transforming growth factor beta3,TGFβ3)对体外培养的羊驼皮肤黑色素细胞表型的影响。本研究在体外培养的羊驼皮肤黑色素细胞中添加不同浓度TGFβ3(50ng/ml、25ng/ml、12.5ng/ml、6.25ng/m1),通过实时监测和检测细胞增殖、毛色相关基因MITF、TYR和TYRP2表达以及黑色素产量的变化。结果表明,在羊驼皮肤黑色素细胞中添加50ng/ml浓度的TGFβ3后,在前30h内对细胞增殖有抑制效果;30h后对细胞增殖有明显的长时程维持细胞数量作用,但对TGFβ3添加的剂量没有依赖性;添加TGFβ3后,黑色素细胞内MITF、TYRP2和TYR在转录水平和蛋白水平的表达量均被下调;黑色素细胞产生黑色素的量也被下调。结果揭示TGFβ3通过对羊驼黑色素细胞内MITE、TYR和TYRP2的表达的影响而调控黑色素的产生,对细胞表型具有重要的影响。缩写:酪氨酸酶相关蛋白2(Tyrosinase Related protein 2,TYRP2)、小眼畸形相关转录因子(Microphthalmia—associtated transcription factor,MITF)和酪氨酸酶(Tyrosinase,TYR)。
(Objective) The previous researches on porcine reproductive and respiratory syndrome virus(PRRSV) and its receptors which based on molecule and cell level were common,but correlational studies on morphology in vivo we...
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(Objective) The previous researches on porcine reproductive and respiratory syndrome virus(PRRSV) and its receptors which based on molecule and cell level were common,but correlational studies on morphology in vivo were *** purpose of this experiment was to investigate the expression pattern of PRRS virus receptors in the central nervous system.(Method) Animal experiment、immunohistochemistry streptavidin-perosidase(IHC-SP) method and double immunofluorescence histochemistry method were utilized for pathological examination、virus distributing detection and the cellular localization of virus receptors.(Result) PRRSV could cause serious nonsuppurative encephalitis to piglets which characterized by hyperemia、degeneration and necrosis of neuron and gliocyte *** were widely founded in hippocampus and spinal cord neuronal cytoplasm,Vimentin were mainly discovered in neuronal and astrocyte cytoplasm,CD163 mainly expressed in neuronal cytoplasm and part of telencephal and hippocampal oligodendrocyte,nonmuscle myosin heavy chain Ⅱ-A(NMHC Ⅱ-A) were widely observed in neuronal cytoplasm.(Conclusion) It could be concluded that the four PRRSV receptors were all existed in the central nervous *** expressed in some nervous cells and functioned differentially during the process of virus *** was the combining with PRRSV and specific target receptors in the main neurocyte of the central nervous system that lead to the neurological symptom of piglets,and these conclusions provide powerful evidences for further explaining of the PRRSV pathogenic mechanism and the combination of PRRSV and its receptors,as well as the developing for more effective vaccines and diagnosis methods.
(目的)衣藻RSP3(radial spoke protein 3)是一个鞭毛结构蛋白,目前有关RSP3相关研究主要集中在衣藻上,而对其在哺乳动物中的同源基因研究甚少。衣藻RSP3在哺乳动物中的同源基因主要表达于中枢神经系统,然而目前尚缺乏对其功能的研究。(...
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(目的)衣藻RSP3(radial spoke protein 3)是一个鞭毛结构蛋白,目前有关RSP3相关研究主要集中在衣藻上,而对其在哺乳动物中的同源基因研究甚少。衣藻RSP3在哺乳动物中的同源基因主要表达于中枢神经系统,然而目前尚缺乏对其功能的研究。(方法)本研究通过构建小鼠RSP3表达载体,转染CHO和293T细胞,应用荧光共定位技术研究小鼠RSP3在哺乳动物细胞中的亚细胞定位。运用子宫内电击转染和免疫荧光染色等技术研究小鼠RSP3在大脑皮层的特殊定位及其对大脑皮层发育的影响。(结果)体外实验结果表明,小鼠RSP3并不直接与微管结合,也不定位到内质网或高尔基体,而是聚集分布于细胞核的周围。利用子宫内电击转染技术,我们成功的标记了室管膜细胞,并发现RSP3特异性分布于室管膜细胞的纤毛上。进一步的研究表明RSP3特异性分布于放射状胶质细胞的纤毛上,并能促进放射状胶质细胞的分裂和大脑皮层的形成。(结论)以上结果表明,RSP3在神经系统发育中具有非常重要的功能,可能与大脑皮层的发育及智力的形成有密切的关系。
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