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Abnormal Social Behavior in Mice Co-exposed to BPA and Pb

Abnormal Social Behavior in Mice Co-exposed to BPA and Pb

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中国畜牧兽医学会家畜内科学分会第七届代表大会暨学术研讨会

作者： Luo Guang-ying,Niu Rui-yan,Wang Chong,Lu Zhao-jing,Wang Jun-dong (Shanxi Key Laboratory of Ecological Animal Science and Environmental Medicine,Shanxi Agricultural University,Taigu,Shanxi,030801,PR of China)

Alerted sociability is the core feature of a variety of human neurological disorders such as autism. Autism is a neurodevelopmental disorder characterized by impaired communication,repetitive behaviors and aberrant reciprocal social *** the etiology remains unclear,strong evidence exists for environmental risk factors,and environmental toxins have been *** A(BPA) and Lead(Pb), informed *** widely used in industry and daily *** and Pb combined exposure models have been established in this *** understand the relationship between combined exposure of BPA and Pb and the diagnostic and associated symptoms of autism,behavioral phenotyping of mouse exposed to BPA and Pb was conducted by investigating general health factors including physical abilities,social *** from our behavioral phenotyping battery indiaed that treated mouse were similar to the control on standardized measures of general ***,sex-specific sociability decreased in male mice co-exposed to BPA and *** finding supported the interpretation that BPA and Pb combined exposure could be the risk factor for autism.

关键词： social behavior BPA Pb sex-specific

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奶牛亚临床酮病对乳房炎发病情况和抗氧化功能的影响

奶牛亚临床酮病对乳房炎发病情况和抗氧化功能的影响

引用

中国畜牧兽医学会家畜内科学分会第七届代表大会暨学术研讨会

作者：薛俊欣张铮臻黄克和张克春南京农业大学动物医学院上海市奶牛研究所

为研究奶牛亚临床酮病与乳房炎的关系以及血液中的酮体成分对奶牛抗氧化功能的影响,以156头健康荷斯坦奶牛为试验动物(SCC0.05);试验组奶牛乳房炎高发期为分娩后3-6周,对照组奶牛乳房炎发病时间为分娩后5-8周,亚临床酮病发病后1-3周内... 详细信息

为研究奶牛亚临床酮病与乳房炎的关系以及血液中的酮体成分对奶牛抗氧化功能的影响,以156头健康荷斯坦奶牛为试验动物(SCC<200.000/ml),共筛选亚临床酮病奶牛69头(1.0mmol/L≤BHBA<2.6mmol/L)作为试验组,根据年龄、胎次、产奶量、泌乳天数配对的69头奶牛作为对照组(血液BHBA<1.0mmol/L),监测乳房炎发病情况并在各组中选取12头奶牛检测血液酮体水平和抗氧化指标。结果表明亚临床酮病奶牛试验期间乳房炎的发病率为43.48%,对照组试验期间乳房炎的发病率为31.88%,但差异不显著(P>0.05);试验组奶牛乳房炎高发期为分娩后3-6周,对照组奶牛乳房炎发病时间为分娩后5-8周,亚临床酮病发病后1-3周内为乳房炎的高发期,占试验组奶牛乳房炎总发病比例的73.33%,试验组和对照组病原菌的组成无显著差异(P>0.05);试验组奶牛血液中T-AOC显著低于对照组(P<0.05),GSH-PX活力、MDA和NO显著高于对照组(P<0.05),血液ACAC浓度与GSH-PX活力呈显著正相关(P<0.05,r=0.595),ACAC浓度与NO呈显著正相关(P<0.05,r=0.655),T-AOC与MDA呈显著负相关(P<0.05,r=0.533)。结果表明奶牛亚临床酮病可以导致乳房炎的发病率升高,但不是主要影响因素,亚临床酮病对乳房炎病原体构成无影响;外周血酮体水平升高导致机体总抗氧化能力的降低并引起机体脂质过氧化反应:谷胱甘肽过氧化物酶在发挥抗氧化功能时具有一定的抵抗力和自我调节能力。

关键词：奶牛亚临床酮病乳房炎抗氧化功能

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Effects of Non-esterified Fatty Acids on the Gluconeogenesis in Bovine Hepatocytes

Effects of Non-esterified Fatty Acids on the Gluconeogenesis...

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中国畜牧兽医学会家畜内科学分会第七届代表大会暨学术研讨会

作者： LI Xin-wei,LI Xiao-bing,BAI Ge,CHEN Hui,DENG Qing-hua,LIU Zhao-xi, ZHANG Liang,LIU Guo-wen,WANG Zhe College of Animal Science and Veterinary Medicine,Jilin University,5333 of Xi'an Road, Changchun,Jilin,130062,P.R.China

Dairy cows experience an increased demand for glucose to support milk ***,negative energy balance is a common condition in peripartum *** response,fat mobilization provides non-esterified fatty acids(NEFAs) for oxidation in the liver to generate *** investigate the effects of NEFAs on gluconeogenesis,the expression and enzyme activity of pyruvate carboxylase(PC) and phosphoenolpyruvate carboxylase(PEPCK) in cultured bovine hepatocytes were evaluated by quantitative polymerase chain reaction and spectrophotometry,*** results showed that PC and PEPCK mRNA levels were marked decreased when the NEFAs concentrations exceeded 0.5 mmol/L and 1.5 mmol/L,*** PC and PEPCK enzyme activity showed significantly decreased when the NEFAs concentrations exceeded 1.5 mmol/L and 0.5 mmol/L, *** findings indicate that high circulating levels of NEFAs inhibit hepatocyte gluconeogenesis, thereby promoting negative energy balance.

关键词： non-esterified fatty acids gluconeogenesis bovine hepatocytes

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Towards the development of a portable sensor based on a molecularly imprinted membrane for the rapid determination of salbutamol in pig urine

Towards the development of a portable sensor based on a mole...

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中国畜牧兽医学会家畜内科学分会第七届代表大会暨学术研讨会

作者： Chunyan Chai,Guoyan Liu*,Feng Li,Xiaofang Liu,Bing Yao,Li Wang School of Agriculture & Biology,Shanghai Jiaotong University,800# Dongchuan Road,Shanghai 200240,China

A new type of conductometric probe based on a molecularly imprinted membrane(MIM) for the detection of salbutamol has been designed and *** probe consists of two parallel screen-printed electrodes(SPE).One of the SPEs was coated with a molecularly imprinted membrane using salbutamol as the template,and the other was modified with a non-molecularly imprinted membrane(N-MIM). Measurements of salbutamol were conducted after the conductometric probe had been connected to a commercial portable ***-sample or successive detections could be easily accomplished by replacing the one-off SPE coated with the salbutamol molecularly imprinted membrane with a new *** conductometric response of the sensor to the concentration of salbutamol displayed a linear correlation over a range from 50 to 280 nM,with a detection limit of 13.5 *** recoveries reached 92.1 -98.3%based on pig urine *** addition,the sensor based on this new type of probe demonstrated high sensitivity and selectivity for salbutamol.

关键词： Salbutamol Molecularly imprinted membrane Urine Conductometry

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ZEA诱导猪卵巢颗粒细胞凋亡机理的研究

ZEA诱导猪卵巢颗粒细胞凋亡机理的研究

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中国畜牧兽医学会家畜内科学分会第七届代表大会暨学术研讨会

作者：朱丽袁莉芸邬静李荣芳文利新袁慧湖南农业大学动物医学院

研究目的:以体外培养的猪卵巢颗粒细胞为模型,通过研究ZEA对其毒性作用,来揭示ZEA对猪生殖系统的毒性作用机理;研究方法:采用机械分离法从1-5mm的卵泡中提取猪卵巢颗粒细胞,Giemsa和结晶紫染色观察细胞形态,免疫细胞化学染色方法检测FSH... 详细信息

研究目的:以体外培养的猪卵巢颗粒细胞为模型,通过研究ZEA对其毒性作用,来揭示ZEA对猪生殖系统的毒性作用机理;研究方法:采用机械分离法从1-5mm的卵泡中提取猪卵巢颗粒细胞,Giemsa和结晶紫染色观察细胞形态,免疫细胞化学染色方法检测FSHR表达来鉴定颗粒细胞,MTT方法测定细胞生长曲线;于细胞对数生长期加入60,90和120μM的ZEA作用24h后,MTT法检测细胞增殖率,氮蓝四唑(NBT)显色法检测SOD活性,硫代巴比妥酸法测定细胞中MDA的含量,间接法检测细胞中GSH-Px活力,TUNEL和Annexin-FITC/PI双染法检测细胞凋亡,分别用荧光探针DCFH-DA和罗丹明123进行活性氧和线粒体膜电位的检测,western blot检测Caspase-3,9蛋白含量,细胞免疫荧光方法测定Caspase-3蛋白的激活与定位以及Z-VAD-FMK及Ac-LEHD-FMK对细胞凋亡的抑制作用;结果:分离提取的猪卵巢颗粒细胞存活率为65%左右,细胞纯度>97%,且细胞生长状况良好,24h-96h为细胞的对数生长期。高剂量ZEA能抑制猪卵巢颗粒细胞增殖,其IC50为90μM;60μM、90μM和120μM ZEA均极显著降低抗氧化酶SOD、GSH-Px活性,升高MDA的含量而引起细胞氧化损伤,呈剂量效应相关性:并呈剂量依赖性诱导细胞凋亡;研究揭示ZEA通过caspase依赖性的线粒体凋亡途径诱导猪卵巢颗粒细胞凋亡,主要表现在:极显著升高细胞内ROS水平,显著性降低线粒体膜电位,激活Caspase-3,9蛋白并诱导Caspase-3从细胞质进入细胞核执行功能,以及caspase抑制剂Z-VAD-FMK与caspase-9抑制剂Ac-LEHD-FMK显著性抑制ZEA所诱导的猪卵巢颗粒细胞凋亡:结论:ZEA抑制猪卵巢颗粒细胞增殖,引起细胞氧化损伤,并经由caspase依赖性的线粒体凋亡途径诱导细胞凋亡。

关键词：玉米赤霉烯酮猪卵巢颗粒细胞氧化损伤细胞凋亡线粒体

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High copper levels promotes broiler hepatocyte mitochondrial permeability transition in vivo and in vitro

High copper levels promotes broiler hepatocyte mitochondrial...

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中国畜牧兽医学会家畜内科学分会第七届代表大会暨学术研讨会

作者： Rongsheng Su~(a1),Rongmei Wang~(b1),Huabin Cao~(c1),Jiaqiang Pan~a,Lijun Chen~a, Chengmei Li~a,Dayou Shi~a,Zhaoxin Tang~(a*) (a College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642, China b College of Ying Dong bio-engineering,Shaoguan University,Shaoguan 510042,China c College of Animal Science and Technology Jiangxi Agricultural University Nanchang 330045, China)

This study was to examine the effects of copper on the mitochondrial non-specific pore.360, one-day-old,healthy Arbor Acres（AA） broilers were fed with different concentrations（11,110,220 and 330 mg/kg） of copper originated from copper sulfate,tribasic copper chloride（TBCC） or copper *** the indicated time point,the mitochondrial permeability transition（MPT） and copper concentration were analyzed. Results showed that,under the same copper concentration,the MPT of broilers fed copper methionine was the greatest,followed by TBCC,then copper *** effects of copper on MPT were time- and dose-dependent. Furthermore,in vitro in the presence of K,5μM Cu could cause permeability transition as compared to 10μM Cu in buffer without *** these results together,we have shown that hepatocellular MPT may be influenced not only by source and concentration of copper or the raising period of broilers,but also by the existence of K.

关键词： Copper Broiler Mitochondria Mitochondrial permeability transition

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High insulin concentrations repress insulin receptor gene expression in calf hepatocytes cultured in vitro

High insulin concentrations repress insulin receptor gene ex...

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中国畜牧兽医学会家畜内科学分会第七届代表大会暨学术研讨会

作者： Zhigang Zhang~1,Xiaobing Li~2,Guowen Liu~2,Yanfei Li~1,Li Gao,Hongbin Wang~1, Ruifeng Gao~2,Zhe Wang~2,Xinglin Zhu~2 (1 College of Veterinary Medicine,Northeast Agriculture University,China 2 College of Animal Science and Veterinary Medicine,Jilin University,China)

The major role of insulin and the insulin receptor(InsR) in the liver is to mediate glucose uptake into hepatocytes to synthesize glycogen and to maintain blood glucose *** this study,we investigated the effects of high insulin concentrations on InsR gene expression in calf hepatocytes cultured in *** the cells were cultured for 72 h,insulin was added to the culture solution at final concentrations of 0,1,10,100 or 1000 nM. InsR mRNA expression was determined by semi-quantitative *** results showed that InsR mRNA expression in hepatocytes,adjusted forβ-actin expression,decreased dose dependently with increasing insulin *** mRNA expression was similar at 1 and 10 nM insulin,but was significantly lower than that in the *** expression was similar at 100 and 1000 nM insulin,but was significantly lower than that in the control.1 and 10 nM insulin *** data suggest that high concentrations of insulin significantly repress InsR mRNA expression in calf hepatocytes,and this inhibition occurs in a dose-dependent *** studies are needed to investigate the mechanisms underlying these effects of insulia

关键词： Insulin Insulin receptor Gene expression Calf hepatocytes Dairy cows

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三聚氰胺致NRK-52e细胞氧化应激与p38MAPK通路介导细胞凋亡的研究

三聚氰胺致NRK-52e细胞氧化应激与p38MAPK通路介导细胞凋亡的研究

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中国畜牧兽医学会家畜内科学分会第七届代表大会暨年学术研讨会

作者：郭成志袁莉芸李荣芳邬静文利新袁慧湖南农业大学动物医学院长沙市 410128

通过研究三聚氰胺导致大鼠肾小管上皮细胞氧化损伤的程度和细胞凋亡及Trolox对肾小管上皮细胞的保护作用探讨其细胞的凋亡通路;将三聚氰胺直接溶解于DMEM培养基中，分别稀释至5mg/ml、4mg/ml、3mg/ml、2mg/ml、1mg//ml、0mg/ml，通过MT... 详细信息

通过研究三聚氰胺导致大鼠肾小管上皮细胞氧化损伤的程度和细胞凋亡及Trolox对肾小管上皮细胞的保护作用探讨其细胞的凋亡通路;将三聚氰胺直接溶解于DMEM培养基中，分别稀释至5mg/ml、4mg/ml、3mg/ml、2mg/ml、1mg//ml、0mg/ml，通过MTT实验确定三聚氰胺对该细胞的半数抑制浓度IC50。根据三聚氰胺对该细胞的IC50选择三个剂量4mg/ml、2mg//ml、1mg/ml作为实验组，并每组都设立向对应的Trolox保护组，其中Trolox的终浓度为5mM。用三聚氰胺和Trolox于培养基中混匀作用于细胞8h后，收集细胞培养液并将细胞裂解收集细胞裂解液。进行相关指标的检测和研究;结果表明，三聚氰胺可以降低抗氧化酶的活性，加速细胞的脂质过氧化程度，破坏了细胞膜的完整性。同时，三聚氰胺还可以促进细胞产生过多的活性氧，这也可能是引起细胞损伤的重要因素之一，同时在加入保护剂Trolox后，三聚氰胺造成的氧化损伤程度有所降低，并且trolox可以通过提高抗氧化酶的活性、清除细胞内氧自由基来保护细胞，使细胞凋亡发生的几率降低;此外，三聚氰胺致NRK-52e细胞凋亡可能与p38MAPK通路有关，阻断p38MAPK可以阻断三聚氰胺所致的细胞凋亡现象。

关键词：三聚氰胺水溶性维生素E NRK-52e细胞氧化损伤 P38MAPK通路细胞凋亡

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Oxidative Stress and Apoptotic Changes of Rat Cerebral Cortical Neurons Exposed to Cadmium in Vitro

Oxidative Stress and Apoptotic Changes of Rat Cerebral Corti...

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中国畜牧兽医学会家畜内科学分会第七届代表大会暨学术研讨会

作者： YUAN Yan,BIAN Jian-chun,LIU Xue-zhong,ZHANG Ying,SUN Ya and LIU Zong-ping (College of Veterinary Medicine,Yang Zhou University,12 East Wen Hui Road,Yangzhou 225009,People's Republic of China)

Cadmium（Cd） can severely damage several organs,including the *** this study, the Cd toxicity on rat cerebral cortical neurons in culture were *** primary cultures of rat cerebral cortical neurons were treated with different concentrations of cadmium acetate（5,10 and 20μM） to investigate its cytotoxic mechanism.A progressive loss in cell viability and increased apoptotic cells were observed in the *** addition,apoptotic morphological changes induced by Cd exposure in cerebral cortical neurons were demonstrated by Hoechst 33258 ***,the ultrastuctural morphology were observed by transmission electron microscopy analysis,the most striking ultrastructural modifications involved distortion of mitochondrial cristae and an unusual ***,elevation of intracellular [Ca] and reactive oxygen species（ROS） levels,depletion of mitochondrial membrane potential （△Ψ） were revealed in a dose-dependent manner during the ***,the activities of catalase（CAT） and superoxide dismutase（SOD） in the living cells increased *** conclusion,exposure of cortical neurons to different doses of Cd led to cellular death,mediated by an apoptotic mechanism,and the apoptotic death induced by oxidative stress may be the chief ***,a disorder of intracellular homeostasis induced by oxidative stress and mitochondrial dysfunction is a trigger of apoptosis in cortical *** the other hand,the cells, which survived Cd action seem to be mediated by their ability to active antioxidant defense systems,since cd induced CAT and SOD activation in these cells.

关键词： cadmium apoptosis oxidative stress cerebral cortical neurons

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Dynamic changes of blood Lipids parameters in Helstein dairy cows from peripartum

Dynamic changes of blood Lipids parameters in Helstein dairy...

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中国畜牧兽医学会家畜内科学分会第七届代表大会暨学术研讨会

作者： He Bao xiang,Du Xianghong,Du Yu Ian,Han Jianbin,Liu Lingjiang,Huangjing (Animal science and technology College,Guangxi University,530005)

To understand the Blood plasma total lipids(TL),triglyceride(TG),total cholesterol(TC),and nonesterified fatty acid(NEFA) concentrations,their dynamic changes and their correlationship before and after of calving in Holstein dairy cows,and their differences between the cows with hyperketoemia(HK) and with normal ketone body levels(NK),a study was conducted in 113 cows in 5 years *** more than 14 cows were chosen every year and the blood samples were collected from the week before calving and week 0-9 after calving once a *** average plasma concentrations of TL,TC,TG,and NEFA were 3.66+ 0.94g/L,3.28±0.87mmol/L,0.17+0.06mmol/L,and 0.63±0.24 mmol/L,*** plasma TL and TC levels increased and NEFA levels decreased from calving day to week 7 *** correlation analysis revealed TL-TC, TC-NEFA,and TL-NEFA had high significant correlationship,their correlation coefficient were 0.8903,-0.6382 and -0.5350 *** HK cows have higher TL,TC TG and NEFA concentrations than NK *** *** cows with HK had mobilized more body fat than NK cows during experimental period. Monitoring and controlling the increasing rate of TL or TC levels during early milking period might be a good method for ketosis prevention.

关键词： cow lipids total lipids NEFA Triglyceride total cholesterol

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