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Effects of selenium form on blood and milk selenium concentrations,milk component and milk fatty acid composition in dairy cows

Effects of selenium form on blood and milk selenium concentr...

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中国畜牧兽医学会家畜内科学分会第七届代表大会暨学术研讨会

作者： Linwu Ran,Xianshi Wu,Xiangzhen Shen,Kechun Zhang,Fei Ren and Kehe Huang a Institute of Nutritional and Metabolic Disorders in Domestic Animals and Fowls, Nanjing Agricultural University,Nanjing 210095,China b College of Animal Science and Technology,Henan University of Science and Technology,Luoyang 471003,China c Institute of Dairy Research in Shanghai,Shanghai 200032,China

BACKGROUND:Human health may be improved if milk with a favorable fatty acid composition and Se concentration is ingested. The present study is to determine howa basal diet supplemented with daily 5 mg Se as Se-enriched yeast(SY) or sodium seienite (SS) affects the fatty acid composition and Se concentration of bovine *** effects of Se form on blood Se concentration, erythrocyte glutathione peroxidase 1(GPxl) activity,serum GPx3 activity and milk yield and component were also studied. RESULTS:Both Se forms,when compared to control group,increased Se concentrations of blood(P < 0.01) and milk(P < 0.01), erythrocyte GPx1 activity(P < 0.05) and milk percentages of polyunsaturated fatty acids(PUFA)(P < 0.05) and c/'s-9,cis-12 linoleicacid(P < 0.05).Cows supplemented with SY had higher Se levels in blood(P < 0.01) and milk(P < 0.01) and percentage of PUFA in milk(P < 0.05) when compared with those supplemented with *** yield,milk component and serum GPx3 activity were not significantly affected by Se form. CONCLUSION:Supplementation of diet with SY appears to be of more benefit than SS in producing favorable milk with high PUFA and Se concentrations.

关键词： selenium form milk Se fatty acid composition dairy cow

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In vitro effect of copper chloride exposure on reactive oxygen species generation and respiratory chain complexes activities of mitochondria isolated from broiler liver

In vitro effect of copper chloride exposure on reactive oxyg...

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中国畜牧兽医学会家畜内科学分会第七届代表大会暨学术研讨会

作者： Rongsheng Su~(a1).Rongmei Wang~(b1).Shining Guo~(a1).Huabin Cao~(c1).Jiaqiang Pan~a. Chengmei Li~a.Dayou Shi~a.Zhaoxin Tang~(a*) (a College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China b College of Ying Dong bio-engineering,Shaoguan University,Shaoguan 510042,China c College of Animal Science and Technology Jiangxi Agricultural University Nanchang 330045,China)

This study is to examine if Cu can act directly on mitochondria or indirectly by producing reactive oxygen species（ROS）,isolated broiler hepatic mitochondria were exposed to different concentration of Cu（10,30,50μM）. Respiratory chain complexes activities,ROS generation,respiratory control ratio（RCR） and mitochondrial membrane potential（MMP） were ***-dependent inhibition of respiratory chain complexes and induction of ROS were observed,which coincided with decreasing of respiratory control ratio（RCR） both with glutamate+malate or succinate. Further investigation indicated that the membrane potential determined by Rhodamine 123 release decreased after CuCl exposure at 30 and 50μ*** additioa effects of the antioxidants NAC（200μM） and GSH（200μM） were studied at 50μM *** results indicate Cu can induce mitochondrial dysfunction in excessive dose and the effect of Cu exposure on respiratory chain is not site-specific,and antioxidants can protect the mitochondrial function by reducing the formation of free radicals.

关键词： copper chloride(CuCl2) Reactive oxygen species Respiratory chain complexes Free radicals Membrane potential

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The roles of toll-like receptor 4 and oestradiol in bovine endometritis

The roles of toll-like receptor 4 and oestradiol in bovine e...

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中国畜牧兽医学会家畜内科学分会第七届代表大会暨学术研讨会

作者： LIU Shan,YANG Zheng-tao,ZUO Zhig-ang,LIU Bo,Zhang Nai-sheng* (College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,Jilin Province)

Objectives:To explore the role of Toll-like receptor 4(TLR4) and immunomodulatory hormones played in bovine ***:The bovine endometrial epithelial cell line BEnEpC was used in the *** BEnEpCs were contaminated by ***,BenEpCs were randomly divided into several groups according to the different concentration of 17-p estradiol(E2) 1treatment and the different stimulation,lipopoly saccharide(LPS) or lipoteichoic acid(LTA).The expressions of TLR4 mRNA and COX-2 were determined by quantitative real-time PCR(qRT-PCR).And PGF and PGE were evaluated by radioimmunoassay. Results:A significant difference was observed in TLR4 expression of bacterial contaminated BEnEpCs among low, middle and high-dose E2 treated group(p < 0.05).And there was also a statistical difference in the relative changes of the PGE2 and COX-2 mRNA expression in BEnEpCs among low,middle and high-dose E2 treated group(p < 0.05).Furthermore,the most significant increase of PGE2 and COX-2 mRNA expression was achieved in the LPS-stimulated BEnEpCs compared with those stimulated with LTA. Conclusions:Our findings suggested that TLR4 was activated in bovine endometritis,and then led to more prostaglandin production as regulation of local inflammatory' response,however,this response was dependent on and influenced by the immunomodulatory hormones.

关键词： Bovine endometritis lipopolysaccharide Toll-like receptor 4 17-βestradiol

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Nonclassic Major Histocompatibility Complex ClassⅠTake Capital Contribution to Reproduction of Dairy Cow

Nonclassic Major Histocompatibility Complex ClassⅠTake Capi...

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中国畜牧兽医学会家畜内科学分会第七届代表大会暨学术研讨会

作者： Lei Shu~1,Shen Zhang~1,Ganzhen Deng~(1*),Yue Wu~1,Mingyue He~1,Beibei Li~1,Chengye Li~1, Kechun Zhnag~3 (1 College of Veterinary Medicine,Huazhong Agricultural University.Wuhan,430070,China 2 Guangming Milk Product Cooperation Ltd,Shanghai,200436,China)

Major histocompability complex(MHC) takes capital contribution to maternal immune response to semiallogenic ***,the evidence of MHC I transcription on maternal peripheral blood monouclear cells(PBMC) and fetal tissues are still incompletely *** pairs of MHC-1 genes were chosen according to their homology and possible *** transcription levels on maternal PBMC from all three trimesters,and transcription levels in fetal tissues were compared to evaluate their contribution to cattle *** results suggested that MHC-1 gene composition and overall transcription levels on PBMC were not similar to that in fetal placenta in last *** geneⅤ(BoLA-NC3* 50201) upregulated throughout pregnancy,maybe act as an immune protective mechanism for pregnant cow;gene IV(BOLA-NC1*) transcription initialized fetus nidation;gene ID(HD15) transcription keeps the pregnancy up:GeneⅡ(MICB) transcription increases the immune response between fetus and dam,and then let fetus *** function of geneⅣsilenced in neonatal ear tissues was pended to further *** geneⅦ,ⅧandⅨshould be synergy with nonclassic gene V(BoLA-NC3*50201) to act as an immune protective mechanism for pregnant cow *** gene VI contribute to pregnancy establishment ant maintenance at first,and then to fetus expulsion *** the classic genes upregulated in neonatal ear tissues,suggest the prevention for fetus *** of heavy chain gene significantly downregulated in all dam and fetal tissues,the effect of classic MHC I genes is restricted.

关键词： diary cow MHC-I reproduction

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Effects of Zearalenone on IL-2,IL-6,and IFN-γ mRNA levels in the splenic lymphocytes of chickens

Effects of Zearalenone on IL-2,IL-6,and IFN-γ mRNA levels i...

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中国畜牧兽医学会家畜内科学分会第七届代表大会暨学术研讨会

作者： Y.C.Wang~1,J.L.Deng~(1,*),S.W.Xu~(2*),X.Peng~3,Z.C.Zuo~4,H. M.Cui~3,Y.Wang~4,Z.H.Ren~4 (1 College of Veterinary Medicine,Sichuan Agricultural University,Yaan,Sichuan 625014,P.R. China,2 Department of Veterinary Medicine,Northeast Agricultural University,Harbin 150030, P.R.China,3Sichuan Key Laboratory of Animal Disease and Human Health,Yaan,Sichuan, 625014,P.R.China Sichuan 625014,P.R.China,4 Sichuan Key Laboratory of environmental hazards disease,Yaan,Sichuan 625014,P.R.China)

Zearalenone(ZEN) is an estrogenic mycotoxin produced by several Fusarium species,which can contaminate food and *** compounds elicit a wide spectrum of toxic effects,including the capacity to alter normal immune *** this study,the in vitro effects of the treatment of ConA-stimulated splenic lymphocytes with ZEN(0-25μg/mL) were *** modulates the expression of IL-2,IL-6,and IFN-γ. The IL-2 levels were up to four fold higher(P<0.05) compared with the levels in the control levels at toxin concentrations of 25μg/mL after 48 h of *** IL-6 levels were critically suppressed at this concentration;these changes were very statistically significant(P<0.05).At lower ZEN concentrations(0.1.0.4 and 1.6μg/mL), the IFN-γlevels changed slightly;however at 6.25 and 25μg/mL,the IFN-γresults reached statistical significance compared with the control levels(P<0.05).These data suggest that ZEN has potent effects on the expression of chicken splenic lymphocytes cytokines at the mRNA level.

关键词： zearalenone mycotoxin immunity chicken cytokines

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Effects of deoxynivalenol on the mRNA levels of IL-2,IL-6,and IFN-γin the splenic lymphocytes of chickens

Effects of deoxynivalenol on the mRNA levels of IL-2,IL-6,an...

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中国畜牧兽医学会家畜内科学分会第七届代表大会暨学术研讨会

作者： Y.C.Wang~(1,2,3),J.L.Deng~(1,2,3*),S.W.Xu~(4,*),X.Peng~(1,2,3),Z.C.Zuo~(1,2,3),H.M.Cui~(1,2,3), Y.Wang~(1,2,3),Z.H.Ren~(1,2,3) (1 College of Veterinary Medicine,Sichuan Agricultural University,Yaan,Sichuan 625014,P.R. China,2 Sichuan Key Laboratory of Animal Disease and Human Health,Yaan,Sichuan,625014, P.R.China,3 Sichuan Key Laboratory of environmental hazards disease,Yaan,Sichuan 625014, P.R.China,4 Department of Veterinary Medicine,Northeast Agricultural University,Harbin 150030,P.R.China)

The immunotoxicity of deoxynivalenol(DON) as well as the induction of cytokines and related genes in the splenic lymphocytes of chicken were *** the present study,the in vitro effects of DON(0-50μg/mL) treatment on ConA-stimulated splenic lymphocytes were examined after 48 *** modulates the expression of IL-2,IL-6,and IFN-γ.The IL-2 mRNA levels at 0.2.3.2,12.5,and 50μg/mL DON were significantly higher(P < 0.05) than the control group(ConA-only stimulated cells group).The highest IL-2 mRNA level was reached at 12.5μg/mL *** IL-6 levels were critically suppressed at all concentrations,and the differences from the control group(P < 0.05) were statistically ***-6 expression was highest at 12.5μg/mL *** IFN-γlevels were significantly higher(P < 0.05) than the control group,and IFN-γexpression was lowest at 0.8μg/mL *** data indicate the potent effects of DON on cytokine mRNA expression in chicken splenic lymphocytes.

关键词： Deoxynivalenol Mycotoxin Immunity Chicken Cytokines

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High insulin concentrations repress insulin receptor gene expression in calf hepatocytes cultured in vitro

High insulin concentrations repress insulin receptor gene ex...

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中国畜牧兽医学会家畜内科学分会第七届代表大会暨学术研讨会

作者： Zhigang Zhang~1,Xiaobing Li~2,Guowen Liu~2,Yanfei Li~1,Li Gao,Hongbin Wang~1, Ruifeng Gao~2,Zhe Wang~2,Xinglin Zhu~2 (1 College of Veterinary Medicine,Northeast Agriculture University,China 2 College of Animal Science and Veterinary Medicine,Jilin University,China)

The major role of insulin and the insulin receptor(InsR) in the liver is to mediate glucose uptake into hepatocytes to synthesize glycogen and to maintain blood glucose *** this study,we investigated the effects of high insulin concentrations on InsR gene expression in calf hepatocytes cultured in *** the cells were cultured for 72 h,insulin was added to the culture solution at final concentrations of 0,1,10,100 or 1000 nM. InsR mRNA expression was determined by semi-quantitative *** results showed that InsR mRNA expression in hepatocytes,adjusted forβ-actin expression,decreased dose dependently with increasing insulin *** mRNA expression was similar at 1 and 10 nM insulin,but was significantly lower than that in the *** expression was similar at 100 and 1000 nM insulin,but was significantly lower than that in the control.1 and 10 nM insulin *** data suggest that high concentrations of insulin significantly repress InsR mRNA expression in calf hepatocytes,and this inhibition occurs in a dose-dependent *** studies are needed to investigate the mechanisms underlying these effects of insulia

关键词： Insulin Insulin receptor Gene expression Calf hepatocytes Dairy cows

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Differential Expression of Bovine Leucocyte Antigen ClassⅠChain-related Genes 2 In preganant Dairy Cows and Neonatal Calves

Differential Expression of Bovine Leucocyte Antigen ClassⅠC...

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中国畜牧兽医学会家畜内科学分会第七届代表大会暨学术研讨会

作者： ~1 Shen Zhang,~1Ganzhen Deng,~1Beibei Li,~1Yue Wu,~1 Mingyue He, ~1 Xiao Wang,~(c1)Chengye Li,~1Changwei Qiu and ~2Kechun Zhang 1 Veterinary Medical College,Huazhong Agricultural University, 430070 Wuhan,China 2 Guangming Milk Product Cooperation Ltd,Shanghai,200436,China

Major histocompatibility complex takes the capital contribution to maternal immune responses to the semiallogenic fetus. As the menber of *** machenism of class I chain-related genes 2(MIC2) on pregnant setup and delivery,till remains to be *** evaluate the possible effect of MIC2 on pregnant establishment and delivery,neonatal ear tissues,fetal placenta, peripheral blood Polymorphonuclear Neutrophils(PMN) of dairy cow in different pregnant stages were collected to analyze MIC2 expression by quantitative ***2 on PMN in dairy cows was downregulated expressed in first and second trimesters and recoveried back to normal level in ***,MIC2 was novel upregulated expressed in neonatal ear tissues and downreguJated expressed in fetal *** results suggested that MIC2 lowly expressed in the first and second trimester of pregnant cows could suppress NKG2D binding MIC2 on PMN and therefore,let embryo escape from maternal immune response. Anabiotic MIC2 expression in peripartum suggested that maternal immune to full grown foetus recover back to normal level which be in favour of *** foetus,novel upregulated expression of MIC2 in tissues was favourible for foetus to escape its ***,downregulated expression in fetal placenta let placenta decoherenced from maternal placentome which was beneficial to delivery.

关键词： MIC2 pregnant dairy cow neonatal calf polymorphonuclear neutrophils fetal placentaxhina

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检测猪圆环病毒2型血清中和抗体阻断ELISA方法的建立及应用

检测猪圆环病毒2型血清中和抗体阻断ELISA方法的建立及应用

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中国畜牧兽医学会家畜传染病学分会第七届全国会员代表大会暨第十三次学术研讨会

作者：黄立平刘长明危艳武陆月华郭龙军温士刚武嘉男吴洪丽中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/猪传染病研究室

采用杂交瘤细胞融合技术,获得了一株具有分泌抗猪圆环病毒2型(PCV2)中和活性的单克隆抗体,利用该株单抗建立了一种阻断ELISA方法。用于检测该病毒感染猪血清中和抗体.该方法先用病毒多克隆抗体包被ELISA板以捕获已知病毒抗原,然后与待... 详细信息

采用杂交瘤细胞融合技术,获得了一株具有分泌抗猪圆环病毒2型(PCV2)中和活性的单克隆抗体,利用该株单抗建立了一种阻断ELISA方法。用于检测该病毒感染猪血清中和抗体.该方法先用病毒多克隆抗体包被ELISA板以捕获已知病毒抗原,然后与待检猪血清抗体及辣根过氧化物酶标记的单抗进行反应,通过测定酶标单抗显示阻断ELISA检测结果。该方法与常规免疫过氧化物酶单层细胞试验(IPMA)对353份试验猪血清样品进行了平行试验,两种方法检测符合率为96.0%;该方法的敏感性为97.9%,特异性为92.4%,与其它几种猪病毒参考阳性血清无交叉反应.用阻断ELISA和血清中和试验检测PCV2血清中和抗体结果表明,两种方法呈显著正相关性(r=0.9970),其敏感性优于血清中和试验。用阻断ELISA对PCV2灭活疫苗免疫与攻毒试验猪血清样品检测结果显示,疫苗接种2 w开始能够检测到病毒中和抗体,4w后血清抗体阳转率达100%;此外,对来自东北3省不同地区送检的703份血清样品进行检测,抗体阳性检出率为73.4%,表明我国养猪场中PCV2感染率较高,该病毒危害严重.本研究建立的阻断ELISA具有操作简便、特异和敏感等特点,可为PCV2流行病学调查及血清抗体检测提供了一种有效技术手段.

关键词：猪圆环病毒2型中和抗体检测单克隆抗体阻断ELISA

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13种消毒剂对规模化蛋鸡场环境微生物MIC、MBC测定

13种消毒剂对规模化蛋鸡场环境微生物MIC、MBC测定

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中国畜牧兽医学会家畜传染病学分会第七届全国会员代表大会暨第十三次学术研讨会

作者：康润敏王红宁高荣周英顺庞平四川大学“动物疫病与食品安全四川省重点实验室”四川大学生命科学学院四川农业大学四川大学生命科学学院

为评价13种消毒剂(白疫杀、百毒杀、过氧化氢、PVP碘、万仑菌清、新洁尔灭.复合酚、新大卫1、碘伏、84、百毒杀S、百毒强杀、菌毒灭)对规模化鸡舍环境微生物(大肠杆菌8099,金黄色葡萄球菌ATCC 6538,枯草杆菌黑色变种芽孢ATCC 9372以及... 详细信息

为评价13种消毒剂(白疫杀、百毒杀、过氧化氢、PVP碘、万仑菌清、新洁尔灭.复合酚、新大卫1、碘伏、84、百毒杀S、百毒强杀、菌毒灭)对规模化鸡舍环境微生物(大肠杆菌8099,金黄色葡萄球菌ATCC 6538,枯草杆菌黑色变种芽孢ATCC 9372以及环境微生物中的11株大肠杆菌,7株金黄色葡萄球菌与3株枯草芽孢杆)的消毒作用,采用肉汤稀释法进行了药物最低抑菌浓度(MIC)和最低杀菌浓度测定(MBC).结果表明:13种消毒剂药物大肠杆菌的MIC最低的依次为是过氧化氢23.4mR/L,PVP碘39-78.1 mg/L以及碘伏39.0-156.3 mg/L;对大肠杆菌MBC最低的依次为过氧化氢46.8 mg/L,PVP碘39-156.3mg/L和碘伏78.1-156.3 mg/L;对金黄色葡萄球菌的MIC最低依次为PVP碘39-78.1 mg/L,过氧化氢46.8 mg/L以及新洁尔灭和新大卫62.5mg/L;对金黄色葡萄球菌的MBC最低的依次为PVP碘39-78.1mg/L,过氧化氢46.8 mg/L,新大卫62.5 mg/L;对芽孢杆菌的MIC最低依次为新洁尔灭62.5mg/L,新大卫62.5mg/L,PVP碘78.1-156.3 mg/L;对芽孢杆菌的MBC最低依次为新洁尔灭62.5 mg/L,过氧化氢93.6 mg/L,新大卫125mg/L。结论:对于规模化鸡场对大肠杆菌抑菌效果最好的的过氧化氢,对金黄色葡萄球菌抑菌效果最好的是PVP碘,对于枯草芽孢杆菌抑菌效果最好的是新洁尔灭。

关键词：消毒剂最低抑菌浓度最小杀菌浓度

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