In this study,experiment was performed with the objective of examining the effects of copper sources and levels on hydrogen peroxide（H2O2） generation of mitochondria from broiler *** were sources of Cu（CuS04 versus...
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In this study,experiment was performed with the objective of examining the effects of copper sources and levels on hydrogen peroxide（H2O2） generation of mitochondria from broiler *** were sources of Cu（CuS04 versus Cu-Met） fed at four dietary Cu levels.288 broilers（Cobb 500,Gallus domesticus） aged 1 day were randomly divided into 8 groups with 36 each and fed with diets as follows:Controls(Cu 11 mg·kg).and high copper(Cu 110 mg·kg,groupⅡ;Cu 220 mg·kg,high copper groupⅢ;Cu 330 mg·kg,high copper groupⅣ) for 60 days under normal condition, three sample-collections were processed at 12 day-old,36 day-old and 60 day-old respectively to investigate the changes of H202 generation of mitochondria from broiler *** result compared with those of control group,H202 generation of mitochondria in high copper group(Cu 110 mg·kg;Cu 220 mg·kg and Cu 330 mg·kg) were increased of two copper sources（P<0.05 or P<0.01）;At the experimental time of the 36th and 60th day,H202 generation of mitochondria from broiler hepatocyte in each group of Cu-Met were increased compared to those of each group of CuS04（P<0.05 or P<0.01）. In addition,the local of H2O2 generation of mitochondria from broiler fed with high dietary copper were mainly concentrated in mitochondrial complex *** results obtained above indicated that high dietary copper could induce oxidized stress damage in liver:at the same level of dietary copper supplement,the organic copper will lead to much faster H2O2 generation velocity of mitochondria from broiler hepatocyte than inorganic *** results can also suggest that mitochondrial complex IV maybe was one of attack target spots under high dietary copper stimulation of organism.
对广西1985～2008年间分离到的26株传染性支气管炎病毒(IBV)分别进行重要基因(S1和N)及血清型的研究,以跟踪和比较流行毒株基因的变异情况及其对抗原性的影响,并对他们相互间的关系进行探讨.对毒株S1基因高变区Ⅰ(HVRⅠ)进行序列测定,并与发表的其它IBV参考株及鸽子分离的冠状病毒株的基因序列进行比较和分析,系统进化关系显示毒株可分为5个基因群,其中有16个分离株属第Ⅰ群,它们与鸽子冠状病毒分离株的氨基酸序列同源性较高,与Massachusetts(Mass)型疫苗株的同源性较低;有15个分离株在33～34位和34～35之间分别有4个和3个氨基酸残基的插入,GX-NN6在33～34位和34～35位之间则均有4个氨基酸残基的插入;X-YL1、GX-NN2与常用的Mass型疫苗株的亲缘关系最近,同属于第Ⅱ群;GX-G、GX-XD与日本同一时期分离的毒株JP Miyazaki 89亲缘关系最近,属于第Ⅲ群:GX-YL6、GX-NN7与欧洲毒株4/91亲缘关系较近,属于第Ⅴ群.本研究的结果表明广西存在着多种类型IBV毒株的流行,毒株S1基因HVRⅠ碱基的突变或插入比较普遍,可导致其氨基酸序列的变化,绝大部分毒株与目前常用的Mass型疫苗株的亲缘关系较低.同一时期的分离株同源性较高,但无明显的地域性差异.对毒株N基因的克隆、序列测定及分析结果发现,26株IBV分离株核蛋白均含有一个长1230bp的ORF,鳊码由409个氨基酸残基组成的多肽,未发现碱基的缺失和插入;系统进化关系显示毒株可分为4个基因群,其中广西的分离株主要分布于第Ⅰ群和第Ⅱ群中,可见广西主要IBV分离株在N基因进化关系上形成了自己独立的进化群。对N基因及其局部功能区序列比较发现,广西IBV分离株与H120疫苗株N蛋白存在广泛的氨基酸变异。广西1985～1988年三株IBV分离株之间N蛋白核苷酸及其推导氨基酸同源性达99%～99.5%,分布于第Ⅲ群中.同时,本研究还发现分离株GX-NN5可能是一株基因重组病毒.应用7个广西代表性分离毒株和3个常用疫苗株分别感染的鸡胚材料免疫兔子的方法制备抗病毒的单因子血清,然后在鸡胚气管环培养(tracheal organ cultures,TOC)上对26个分离株进行病毒交叉中和试验.结果广西26个分离毒株可分为7个血清型,目前在广西流行的IBV野毒株之间以及其与疫苗株间的抗原性存在很大程度的差异,分属不同的血清型,新的血清型不断出现而且他们在分离株中所占的比例逐年增加;基因分型和血清分型结果之的比较发现,他们之间并不完全一致。研究的结果为及时跟踪广西野毒株重要基因和抗原的变异以及研发更有效疫苗奠定了科学依据.
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