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High-level expression of TSOL18 oncosphere antigen of Taenia solium in Pichia pastoris in a 50 L bioreactor

High-level expression of TSOL18 oncosphere antigen of Taenia...

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中国畜牧兽医学会家畜寄生虫学分会第六次代表大会暨第十一次学术研讨会

作者： Hou Junling,Luo Xuenong,Wang Ying,Gao Hui-li,Fang Yongxiang,Gong Wei,Zhang Shaohua, Liu Zhenyong,Cai Xuepeng~* Key Laboratory of Veterinary Parasitology of Gansu Province,Ministry of Agriculture Key Laboratory of Veterinary Public Health,State Key Laboratory of Veterinary Etiological Biology,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,the People's Republic of China

Taenia solium oncosphere is a hexacanth larva that migrates from the intestine to internal tissues or organs within the intermediate ***18 is an antigen expressed during the developmental stage of the oncosphere,and it is regarded as a candidate antigen for development of *** this study,Pichia pastoris GS115,containing TSOL18,was cultured in the synthetic basal salts medium(BSM) in a 50 L *** conditions comprised an air flow rate of 0.208 wm,dissolved oxygen(DO) above 50% and a temperature of 28℃.The cell growth indexes were evaluated by measuring DO and wet cell weight (WCW).When WCW achieved 96 g/L,glycerol feed medium(2800 ml) was fed into the bioreactor at a flow rate of 4.05 ml/*** the glycerol was depleted(the WCW reached 233 g/L),the cells were starved for 2 ***,methanol induction was conducted with a flow rate of 0.4 ml/min for 13 h, 0.6 ml/min for 23.5 h,and 0.8 ml/min for 35.5 h,*** induction,the expression of the recombinant TSOL18 protein in the supernatant of the broth was *** results showed that the yield of the recombinant TSOL 18 protein was 5.4 g/*** the recombinant TSOL18 protein in supernatant was evaluated by ELISA,high reactivity with positive sera from pigs challenged by *** eggs was *** indicates that the biological activity of the protein is appropriate for use as a vaccine against cysticercosis.

关键词： High-level expression of TSOL18 oncosphere antigen of Taenia solium in Pichia pastoris in a 50 L bioreactor

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Identification of Toxoplasma gondii genes expressed during infection by in vivo-induced antigen technology(IVIAT)

Identification of Toxoplasma gondii genes expressed during i...

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中国畜牧兽医学会家畜寄生虫学分会第六次代表大会暨第十一次学术研讨会

作者： Tao Qing~(1,2),Xiao Jang~(1,2),Wang Yifan~(1,2),Zhao Junlong~(1,2,*) State Key Laboratory of Agricultural Microbiology,Huazhong Agricultural University,Wuhan 430070, Hubei,China College of Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070, Hubei,China

In vivo induced antigen technology(IVIAT) is an effective immunological technique that screens the pathogene antigens expressed during *** applied IVIAT on Toxoplasma gondii and identified fourteen reactive *** identified genes contained in positive clones showed homologies with genes encoding proteins of different functions involving cell invasion(PI,CyP,GRA5 and MICH),ion/protein binding(CaM and LRRP),biosynthesis and metabolism(D3PD and EF1),and six known hypothetical *** expression of five genes including calmoduilin(CaM) gene JK483883,dense granule protein 5(GRA5) gene JK483900,microneme protein 11(MIC11) gene JK483892,Cyclophilin(CyP) gene JK483893 and serine proteinase inhibitor(PI) gene JK483886 identified by IVIAT were confirmed in tachyzoites in mice comparing to that in the culture condition by real-time *** length ORFs of the five genes were amplified and *** functions of the five identified genes were related with host cell invasion of Toxoplasma gondii suggesting that the ivi antigens may play very important roles in pathogenicity of the parasite during *** genes identified by IVIAT may support candidate molecules for potential therapies and preventative measures against Toxoplasma gondii.

关键词： IVIAT Identification of Toxoplasma gondii genes expressed during infection by in vivo-induced antigen technology

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Acaricidal activity of extract from Eupatorium adenophorum against the Psoroptes cuniculi and Sarcoptes scabiei in vitro

Acaricidal activity of extract from Eupatorium adenophorum a...

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中国畜牧兽医学会家畜寄生虫学分会第六次代表大会暨第十一次学术研讨会

作者： Nong Xiang~(a,b),Fang Chunlin~c,Wang Jiahai~a,Gu Xiaobin~a,Yang Deying~a,Liu Tianfei~d,Fu Yan~a, Zhang Runhui~a,Zheng Wanpeng~a,Peng Xuerong~e,Wang Shuxian~a,Yang Guangyou~(a,*) a Department of Parasitology,College of Veterinary Medicine,Sichuan Agricultural University,Ya'an 625014,China b College of Chemical and Life Sciences,Leshan Normal University,Le'shan 614004,China c Sichuan Engineering Research Center For New Veterinary Drug,Cheng'du 611130,China d College of Animal Science & Technology,Sichuan Agricultural University,Ya'an 625014,China e College of Life and Basic Sciences,Sichuan Agricultural University,Ya 'an 625014,China

The possible acaricidal activity of Eupatorium adenophorum was analyzed using extracts created by water decocting,ethanol thermal circumfluence,and steam *** toxic effect of each extract was tested against Psoroptes cuniculi and Sarcoptes scabiei in *** thermal circumfluence extract had strong toxicity against mites,killing all Sarcoptes scabiei at 0.5 g/ml（w/v） concentration,while 1 g/ml extract was also found to kill all Psoroptes cuniculi within a four hour ***,0.25 g/ml and 0.5 g/ml concentration of extract had strong toxicity against Sarcoptes scabiei,with median lethal time (LT) values at 0.82 h and 0.798 h,respectively.0.5 g/ml and 1 g/ml showed strong acaricidal action against Psoroptes cuniculi,the LT values were 0.93 h and 1.29 h,*** median lethal concentration(LC) values were 0.27 g/ml for Scabies mite and 0.64 g/ml for Psoroptes cuniculi in 1 h. The results indicate that Eupatorium adenophorum contains potent acaricidal ingredients that could be developed and utilized to treat skin infections.

关键词： Acaricidal activity of extract from Eupatorium adenophorum against the Psoroptes cuniculi and Sarcoptes scabiei in vitro

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PCR-RFLP Analysis of 18S rDNA:Differentiation of Echinococcus multilocularis,*** and ***

PCR-RFLP Analysis of 18S rDNA:Differentiation of Echinococcu...

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中国畜牧兽医学会家畜寄生虫学分会第六次代表大会暨第十一次学术研讨会

作者： Yan Hongbin~1,Lou Zhongzi~1,Li Hongmin~1,Shi Wangui~2,Liu Quanyuan~2,Zhan Fang~2,Liu Xu~2,Ni Xingwei~1,Jia Wanzhong~(1*) 1 State Key Laboratory of Veterinary Etiological Biology,Key Laboratory of Veterinary Parasitology of Gansu Province,Key Laboratory of Veterinary Public Health of Ministry of Agriculture,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China 2 Gansu Provincial Control for Animal Disease Control and Prevention,Lanzhou 730046,China

Background In Shiqu County of the Qinghai-Tibet plateau,many wild and domestic mammals are involved in the transmission cycles of Echinococcus *** the adult stage,*** from the foxes is morphologically similar to *** addition,it is difficulty to distinguish ***,E. multilocularis and *** based on morphology of their *** and *** are major sources for human *** is necessary to establish some methods for identifying species. In this study,three species of Echinococcus genus(***,*** and ***) were characterized using a polymerase chain reaction-based restriction fragment length polymorphism technique (PCR-RFLP). Methods The complete 18S rRNA gene was amplified from individuals of each of the three species with PCR and then digested separately with one restriction endonuclease,and the fragments were separated by conventional agarose gel electrophoresis(PCR-RFLP). Results PCR-RFLP produced characteristic patterns for complete 18S rDNA sequences of *** and *** species,but no characteristic patterns were produced for ***. Conclusion The present study indicates the usefulness of the PCR-RFLP of 18S rDNA for systematic, epidemiological and diagnostic ***,this method offers an objective,simple and rapid approach for the discrimination of *** and *** and for study of other parasite complexes.

关键词： Echinococcus Species identification Characterisation PCR-RFLP Ribosomal DNA

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The ribosomal DNA gene ITS sequence analysis and PCR-RFLP analysis of roundworms from captive Pan troglodytes,Hylobates hoolock and Macaca mulatt

The ribosomal DNA gene ITS sequence analysis and PCR-RFLP an...

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中国畜牧兽医学会家畜寄生虫学分会第六次代表大会暨第十一次学术研讨会

作者： Cheng Zigang~(a,1) Niu Lili~(b,1) Wang Qiang~b Deng Jiabo~b Yu JianQiou~b,Yu Xinming~b,Yan Huijuan~b, Zhang Zhihe~c,Xie Yue~a,Fu Yan~a,Gu Xiaobin~a,Yang Deying~a,Wang Shuxian~a,Pen Xuerong g~d, Yang Guangyou~(a*) a Department of Parasitology,College of Veterinary Medicine,Sichuan Agricultural University,Ya'an 625014,China b Chengdu Zoological Garden,Chengdu 610041 c The Sichuan Key Laboratory for Conservation Biology on Endangered Wildlife-Developing toward a State Key Laboratory for China,Chengdu Research Base of Giant Panda Breeding,Chengdu,Sichuan 610081,China d College of Life and baisc Science,Sichuan Agricultural University,Ya'an 625014,China

To identify the taxonomic status of Ascaris,which parasitic in captive Pan troglodytes, Hylobates hoolock and Macaca mulatt,polymerse chain reaction（PCR） was used to amplify the Ribosomal internal transcribed spacer（ITS） genes of the three Ascaris species were amplified and the sequence alignment was done with the related gene sequences from *** length of the ITS sequences of worms from ***,*** and *** was 881bp,882bp and 878bp,*** the three species,the length of 5.8S and ITS-2 gene was 157bp and 274bp,the sequences are the same to each *** length of ITS-1 is 450bp,451bp and 447bp,***,it exists length and a few base *** sequence similarity with the ITS of Ascaris lumbricoides（AB571299） reported before is 99.43%-99.66%,and 99.55%-100.00%with the ITS of Ascaris suum（AB571302）.The type of enzymeigestion band was similar to that of *** by PCR-RFLP and restriction endonuclease HaeⅢ, the result showed that Ascaris from ***,*** and *** are ***.

关键词： The ribosomal DNA gene ITS sequence analysis and PCR-RFLP analysis of roundworms from captive Pan troglodytes,Hylobates hoolock and Macaca mulatt DNA

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Prevalence of Coccidial Infection in Cattle in Shaanxi Province,Northwestern China

Prevalence of Coccidial Infection in Cattle in Shaanxi Provi...

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中国畜牧兽医学会家畜寄生虫学分会第六次代表大会暨第十一次学术研讨会

作者： Yu Sanke,Gao Man,Huang Ni,Jiang Yanqing,~* Lin Qing,~* Zhao Guanghui College of Veterinary Medicine,Northwest A & F University,Yangling,Shaanxi Province 712100,PR China

Coccidial infection in cattle mainly occurred in calves because of the incomplete immune system, intensive feeding and management systems,with severe diarrhea and even *** recovered cattle would present to be low production of milk and *** catile,one of the most important beef varieties in China,is mainly raised in Shaanxi province,and it also has been introduced to many other provinces of ***,prior to the present study,there has been no published paper on coccidial infection of Qinchuan *** the present study,the prevalence of coccidial infection was investigated in dairy cattle and Qinchuan cattle in Shaanxi province,northwestern China.A total of 228 cattle(83 and 145 for dairy cattle and Qinchuan cattle,respectively) feces samples were collected and examined by flotation technique using saturated *** oocysts were seen in 90 faecal samples,with overall prevalence of 34.94%(29/83) and 42.07%(61/145) for dairy cattle and Qinchuan cattle.12 Eimeria(namely,E. subspherica,***,***,***,***,***,***,***, ***,***,***,***) and one Isospora(only found in Qinchuan cattle) species were identified according to the time of sporulation Coudert's key and morphological features of sporulated oocysts,with the most common species of ***,***,*** than 80%and 90%samples for two breeds were infected with two and more *** coccidial oocysts per gram(OPG) were ranged from 0 to 2150,with average of *** results suggested that integrated strategies should be implemented to prevent and control coccidial infection in cattle in this province.

关键词： Prevalence of Coccidial Infection in Cattle in Shaanxi Province,Northwestern China

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Genetic variability in three mitochondrial DNA regions of Spirometra erinaceieuropaei spargana in the Hunan Province of China

Genetic variability in three mitochondrial DNA regions of Sp...

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中国畜牧兽医学会家畜寄生虫学分会第六次代表大会暨第十一次学术研讨会

作者： Liu W~(1,2),Liu GH~1,Li F~(1,2),He DS~2,Wang T~1,Sheng XF~1,Zeng LD~1,Yang FF~1,Liu Y~(1*) 1 College of Veterinary Medicine,Hunan Agricultural University,Changsha,Hunan Province 410128, PR China: 2 Hunan Professional Technical College of Biology,Machinery and Electronics,Changsha Hunan province 410128 PR China:

Mitochondrial(mt) genome sequences are very useful for investigating population genetic structures,systematics,and phylogenetics of *** the present study,genetic variation among isolates of Spirometra erinaceieuropaei spargana from different geographical locations in Hunan province, China,were characterized by three mt genes encoding cytochrome c oxidase subunit 3(cox3),NADH dehydrogenase subunits 1 and 4(nad1 and nad4),*** three mtDNA regions were amplified separately from genomic DNA of individual sparganas by polymerase chain reaction(PCR) and the amplicons were sequenced *** sequences of pcox3,pnad1,and pnad4 were 541,607,and 847 bp in length,*** A+T contents of the sequences were 68 39-68.76%(pcox3),63.76-64.91% (pnad1),and 67.18-67.77%(pnad4),*** variations between *** from different regions were 0.0-1.5%for pcox1,0.0-2.8%for pnad1,and 0.0-2.7%for *** analysis using neighbor joining(NJ),maximum likelihood(ML),and maximum parsimony(MP) methods inferred that spargana isolates in Hunan province clustered into two main clades:isolates from Yiyang and Shaoyang clustered together in one clade,with a high bootstrap value(>50%),and those from other origins grouped together in another *** findings demonstrated the usefulness and attributes of the three mtDNA sequences for population genetics studies of ***,the results of the present study also have implications for the diagnosis and control of *** infections in human and animals.

关键词： mtDNA Genetic variability in three mitochondrial DNA regions of Spirometra erinaceieuropaei spargana in the Hunan Province of China

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Soil contamination of Toxoplasma gondii oocysts in pig farms in central China

Soil contamination of Toxoplasma gondii oocysts in pig farms...

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中国畜牧兽医学会家畜寄生虫学分会第六次代表大会暨第十一次学术研讨会

作者： Du.Fen~(a,b),Zhang.Qingli~(a,b),Yu.Qian~(a,b),Hu.Ming~(a,b),Zhou.Yanqin~(b,c),Zhao.Junlong~(a,b,*) a State Key Laboratory of Agricultural Microbiology,Huazhong Agricultural University,Wuhan 430070, Hubei,PR China b College of Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070,Hubei,PR China c Key Laboratory Preventive Veterinary of Hubei Province,Huazhong Agricultural University,Wuhan 430070,Hubei,PR China

Toxoplasmosis in pigs is a large threat to pig industry as well as pork *** pigs become infected by ingestion of oocysts from contaminated environment(soil,water and feed) or infected tissues of animals *** studies were conducted to evaluate the relationship between Toxoplasma gondii infection in pigs and soil contamination status of *** oocysts from 12 farm pigs,6 with high and low density of cats.A total of 2277 sera were collected to determine the presence of IgG antibodies against *** using *** were found in 773 pigs,including 453 of 1238 (36.59%) samples from pig farms with high cat density and 220 of 1039(21.17%) samples from pig farms with low cat density(P<0.01).The presence of *** oocysts in soil were determined by PCR and loop-mediated isothermal amplification(LAMP).*** DNA was found in 11 farms except 1 farm exposed to low cat ***(21.05%) and 36(37.89%) of 95 scil samples were *** positive by PCR and LAMP,respectively(0.01

关键词： Soil contamination of Toxoplasma gondii oocysts in pig farms in central China

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Difference analysis on pathology and immunology of natural hosts infected with schistosoma japonicum

Difference analysis on pathology and immunology of natural h...

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中国畜牧兽医学会家畜寄生虫学分会第六次代表大会暨第十一次学术研讨会

作者： Yang Jianmei,Fu Zhiqiang,Feng Xingang,Shi Yaojun,Liu Jinming,Yuan Chunxiu,Lin Jiaojiao Shanghai Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Key Laboratory of Animal Parasitology,Ministry of Agriculture of China,Shanghai 200241,China

Yellow cattle and water buffalo are two of the most important natural hosts for *** in *** observation has revealed that yellow cattle are more suited to the development of S. japonicum than water *** this study,the development of schistosomes in yellow cattle and water buffalo,as well as the pathological and immunological differences between two hosts,were compared pre and post infection with *** worm length（p<0.05）and the worm recovery rate（p<0.01） had siginificant difference between yellow cattle and water *** percentage of paired worms from water buffalo were significantly fewer than those from yellow cattle（p<0.01）.There were many white egg granulomas in the livers of yellow cattle,but fewer were observed in water buffalo artificially infected with schistosomes for 7 *** livers of infected yellow cattle contained significantly increased accumulation of inflammatory cells,and the schistosome eggs were surrounded with large amounts of eosinophil *** contrast,no hepatocyte swelling or lymphocyte infiltration,and fewer white blood cells,were observed in water *** percentage of CD4T cells was higher in yellow cattle than in water buffalo;in contrast,the percentage of CD8T cells was higher in water buffalo than in yellow cattle from pre-infection to 7 weeks post infection with *** CD4T cell proportion decreased in yellow cattle,and the CD8T cell proportion increased in water buffalo;the CD4/CD8 ratios were decreased in both species after challenge with *** yellow cattle,the IFN-γlevel was higher in pre-infected cattle and 4 weeks post infection,then it decreased significantly and was at a very low level 7 weeks post *** IL-4 level was very low in pre-infected cattle and 2 weeks post infection;it was expressed at a low level at 4 weeks and continued to increase at 7 weeks post challenge with *** water buffalo,the IFN-γlevel was low in pre-infected ani

关键词： Difference analysis on pathology and immunology of natural hosts infected with schistosoma japonicum

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In vitro infection of bovine and ovine red blood cells with Theileria annulata schizonts

In vitro infection of bovine and ovine red blood cells with ...

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中国畜牧兽医学会家畜寄生虫学分会第六次代表大会暨第十一次学术研讨会

作者： Youquan Li~(1-6),Guiquan Guan~(1-6),Zhijie Liu~(1-6),Jifei Yang~(1-6),Qingli Niu~(1-6),Ze Chen~(1-6),Jianxun Luo, Hong Yin~(1-6*). 1 State Key Laboratory of Veterinary Etiological Biology 2 Key Laboratory of Veterinary Parasitology of Gansu Province 3 Engineering Research Center of Biological Detection of Gansu Province 4 Key Laboratory of Epizootic Diseases of Grazing Animal of Ministry of Agriculture 5 Key Laboratory of Veterinary Public Health of Ministry of Agriculture 6 Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Science,Xujiaping 1, Lanzhou,Gansu,730046,P.R.China

Theileria annulata,the causative agents of tropical theileriosis,is protozoan parasites that cause lymphoproliferative diseases of *** vivo,parasitized cells undergo clonal expansion and infiltrate both lymphoid and non-lymphoid tissues of the infected *** this study,in vitro infection of bovine and ovine red blood cells with Theileria annulata schizonts is firstly carried *** experiments showed that Theileria annulata schizonts can infect bovine red blood cells from cattle 2 and cattle 30 in vitro,and also infect ovine red blood cells from sheep 2 and sheep 82 in ***,T annulata schizonts can infect both bovine red blood cells and ovine red blood cells in *** proliferation of Theileria annulata schizonts-infected lymphocytes is obviously suppressed by bovine red blood cells and ovine red blood cells. T,annulata schizonts could provide an intriguing model for the study of parasite-host interactions in vitro in the future.

关键词： In vitro infection bovine red blood cells ovine red blood cells Theileria annulata schizonts

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