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检索条件"任意字段=中国畜牧兽医学会家畜寄生虫学分会第六次代表大会暨第十次学术研讨会"

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Prevalence and identification of human Cryptosporidium *** China:unique *** population and first report of C. felis

Prevalence and identification of human Cryptosporidium *** C...

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中国畜牧兽医学会家畜寄生虫学分会第六次代表大会暨第十次学术研讨会

作者： Rongjun Wang~a,Huili Zhu~a,Longxian Zhang~(a,*),Yaoyu Feng~b,Fuchun Jian~a,Chengshen Ning~a,Meng Qi~a,Yang Zhou~a,Kanda Fu~c,Yaqiang Wang~c,Yanru Sun~a,Qiang Wang~a,Jiacheng Li~a, Hailiang Dang~a,Lihua Xiao~d (aThe College of Animal Science and Veterinary Medicine,Henan Agricultural University,Zhengzhou 450002,China bSchool of Resource and Environmental Engineering,East China University of Science and Technology,Shanghai 200237,China cInspection division of Henan university Huaihe hospital,Kaifeng 475000,China dDivision of Parasitic Diseases,National Center for Zoonotic,Vector-Borne and Enteric Diseases,Centers for Disease Control and Prevention,Atlanta,GA 30341)

A total of 9033 stool specimens from three representative areas were examined between June 2007 and December 2008 to assess the prevalence of human cryptosporidiosis in Henan Province in *** were concentrated by forma... 详细信息

关键词： Prevalence and identification of human Cryptosporidium spp.in China:unique C.hominis population and first report of C felis

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Altered splenic microRNA expression profile in mice infected with Toxoplasma gondii RH strain

Altered splenic microRNA expression profile in mice infected...

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中国畜牧兽医学会家畜寄生虫学分会第六次代表大会暨第十次学术研讨会

作者： Quan Liu~1,Liming Shang~1,Ze Wang~1,Hongtao Jin~1,Hai Lian~1,Chen Chen~1,Zhiping Xia~1,XingQuan Zhu~2 (1Laboratory of Parasitology,Veterinary Institute,Academy of Military Medical Sciences,1068 Qinglong Road, Changchun 130062,China 2Laboratory of Parasitology,College of Veterinary Medicine,South China Agricultural University,483 Wushan Street, Tianhe District,Guangzhou 510642,Guangdong Province,China)

MicroRNAs(miRNAs) are a new class of endogenous non-coding RNAs that play critical roles in a wide spectrum of biological processes,and participate in host-pathogen *** this study,splenic miRNAs expression in mice infected with Toxoplasma gondii RH strain was analyzed using locked nucleic acid-based miRNA arrays, further validated by quantitative real-time *** results indicated that there were 39 downregulated miRNAs and 36 upregulated miRNAs,which were classified into three functional groups,relating to immune response, cell proliferation and *** function analysis of altered microRNA expression suggests that *** develops sophisticated mechanisms to cause toxoplasmosis,and miRNAs may provide a basis for exploring the molecular pathogenesis of *** infection.

关键词： Toxoplasma gondii microRNA microarray quantitative real-time RT-PCR

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The protective effect of a Toxoplasma gondii SAG1 plasmid DNA vaccine in mice is enhanced with IL-18

The protective effect of a Toxoplasma gondii SAG1 plasmid DN...

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中国畜牧兽医学会家畜寄生虫学分会第六次代表大会暨第十次学术研讨会

作者： Quan Liu~(a,*),Limin Shang~a,Jingtao Men~a,Shengyan Gao~a,Hai Lian~a,Ze Wang~a,Yujia Zhai~a,Zhiping Xia~a,Xing-Quan Zhu~(b,*) (aLaboratory of Parasitology,Veterinary Institute,Academy of Military Medical Sciences,Changchun 130062,Jilin Province,China bDepartment of Parasitology,College of Veterinary Medicine,South China Agricultural University,483 Wushan Street,Tianhe District,Guangzhou 510642,Guangdong Province,China)

The protozoan parasite Toxoplasma gondii is the causative agent of a worldwide zoonosis and development of vaccine would be of great medical and veterinary *** the present study,two recombinant plasmids pcDNA /TgSAG1 and pVAX/mIL-18 containing *** SAG1(TgSAG1) and murine IL-18 were evaluated for their ability to protect mice against *** *** were given 2 intramuscular immunizations 3 weeks apart,and challenged with *** 3 weeks *** animals vaccinated with pcDNA/TgSAG1 alone or with pVAX/mIL-18 developed specific anti-TLA(*** lysate antigen) ELISA antibody and T lymphocyte proliferation *** of pVAX/mIL-18 significantly increased the production of IFN-γand IL-2,indicating that IL-18 enhances the Th1-dominant immune *** experiments showed that co-injection of pVAX/mIL-18 significantly increased the survival rate(60%),compared with pcDNA/TgSAG1 alone providing partial protection(40%) against challenge(P<0.05).These results indicate that IL-18 may be an effective adjuvant for a toxoplasmosis vaccine.

关键词： Toxoplasma gondii TgSAG1 IL-18 Immunity

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Immunoproteomic analysis of whole proteins from male and female adult Haemonchus contortus

Immunoproteomic analysis of whole proteins from male and fem...

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中国畜牧兽医学会家畜寄生虫学分会第六次代表大会暨第十次学术研讨会

作者： Feng Bin Yan,Li Xin Xu,Li Heng Liu,Ruo Feng Yan,Xiao Kai Song,Xiang Rui Li~* (College of Veterinary Medicine,Nanjing Agricultural University,Nanjing,Jiangsu 210095,PR China)

Whole proteins of male and female adult Haemonchus contortus were analysed by immunoproteomic *** 662 and 680 spots were detected on proteome maps of male and female nematodes,respectively, stained with Coomassie brilliant blue *** were 609 shared *** 193 and 196 spots were recognised on Western blot maps of male and female nematodes,respectively,using antiserum from naturally infected goats as the source of primary *** were 129 gender-specific spots in male nematodes and 132 in ***-three shared immunogenic spots were identified by MALDI-TOF or MALDI-TOF-TOF mass spectrometry. These proteins included glutamate dehydrogenase(GDH),homologues of dim-1,actin,globin-like excretory /secretory protein F6,glutathione S-transferase(GST),ATPase and glyceraldehyde-3-phosphate dehydrogenase. GDH and GST have been identified as immunogenic proteins of *** previously,whereas the other proteins are newly recognised immunogenic proteins in this nematode.

关键词： Haemonchus contortus Adult nematode Gender-specific proteins Immunoproteome

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The Construction of Rabbit Immunity Model against Haemaphysalis longicornis and Its Effects on *** Adult Growth

The Construction of Rabbit Immunity Model against Haemaphysa...

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中国畜牧兽医学会家畜寄生虫学分会第六次代表大会暨第十次学术研讨会

作者： XIE Jun-ren,LIU Guang-yuan~*,TIAN Zhan-cheng (Key Laboratory of Veterinary Parasitology of Gansu Province,State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterynary Parasitology lf Gansu Province,Key Laboratory of Grazing Animal Diseases of Ministry of Agriculture,Key Laboratory of Veterynary Public Health of Ministry of Agriculture,Lanzhou Veterinary Research Institute,CAAS,Lanzhou 730046)

In this study the rabbit immunity model against Haemaphysalis longicornis was constructed by infestation with *** adult quantitatively every two *** results of ELISA showed that the antibody was positive three weeks post the first infection,with the antibody level rising with the increasing of infection and reached the peak at the 11th *** resistance of rabbit against *** significantly influenced the *** plump-blooding *** antibody level was positively correlated to the bloodsucking period and the mortality of female *** adult and negatively correlated to the weight of plump-hlooding female *** results indiated that the salivary gland is one of the main organs in the anti- *** *** maintenance of the antibody level was enough to make *** to complete a generation,which satisfied the experiment require- *** it is an ideal model for *** immunity positive control *** the same time,the specific antibody level in the serum can be one of the important indexes to evaluate whether the animal resistance to *** is strong or weak.

关键词： Haemaphysalis longicorns adult immunity model growth

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Detection of Neospora caninum in aborted bovine fetuses and dam blood samples by nested PCR and ELISA and seroprevalence in Beijing and Tianjin,China

Detection of Neospora caninum in aborted bovine fetuses and ...

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中国畜牧兽医学会家畜寄生虫学分会第六次代表大会暨第十次学术研讨会

作者： L.Yao,N.Yang,Q.Liu~*,M.Wang,W.Zhang,W.F.Qian,Y.F.Hu,J.Ding (Key Laboratory of Zoonosis of Ministry of Agriculture and National animal Protozoa Laboratory,College of Veterinary Medicine, China Agricultural University,Beijing 100193,China)

*** infection is a significant cause of abortion in *** investigated the tissue distribution of Neospora caninum in aborted bovine fetuses and dam blood samples by a nested PCR assay,and compared the nested PCR with ELISA in the diagnosis of *** *** six aborted fetuses and 813 blood samples were collected from eight dairy herds in Beijing(n=212) and Tianjin(n=601),*** fetuses(57.7%) were tested *** positive by the nested *** DNA was detected from the brain of 52%,kidneys of 22%,skeletal muscle of 18%,and heart of 4%of the aborted *** PCR positive cases(55%,11/20) was higher than seropositive cows(40%,8/20) in a subset of 20 fetuses,but the PCR results of blood samples of the 20 cows were all *** seroprevalence of the 813 samples was 15.5%(43.4%of samples from Beijing,5.7%of samples from Tianjin),compared to the PCR positive blood samples of 0.9%.Our study showed that the nested PCR is a valuable diagnostic tool for the primary diagnosis of *** in aborted fetuses,while ELISA is the preferred assay for testing blood samples collected from *** two assays are complementary in determining whether abortions are associated with *** infection in cattle.

关键词： Neospora caninum ELISA Nested PCR Abortion Bovine fetus

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cDNA Microarray Analysis of the 1080 Genes Expression Profiling during different Segments in Moniezia expansa

cDNA Microarray Analysis of the 1080 Genes Expression Profil...

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中国畜牧兽医学会家畜寄生虫学分会第六次代表大会暨第十次学术研讨会

作者： Wenjuan Zhao,Hui Zhang,Xinwen Bo~*,Xinhua Wang,Lichao Kang (a Genetic Engineering Laboratory,The Breed & Biotechnology Key Laboratory of Sheep in XinJiang Bingtuan 832000,P.R.China)

The gene expression profilings in different proglottids of *** were analysed with cDNA microarray of 1080 ESTs from the worm.A total of 4,056 spots including full length and partial complementary DNAs representing novel,known,and control genes were *** showed that 55 genes expression in immature proglottids,134 genes in mature proglottids and 103 genes in gravid proglottids were up-regulated,and that 7 genes in immature proglottids,68 genes in mature proglottids and 78 genes in gravid proglottids were down-regulated compared with control(scolex-neck proglottids).Major function of these genes are about metabolism,transport,protein biosynthesis,transcription factors,apoptosis,cell differentiation,cell communication and nucleic acid binding protein -related *** changes of some genes,such as UBE2A,Carβ,RAD51,DAZ,PKAc and 2 unknown genes were confirmed by Real-time quantitative Polymerase Chain Reaction(RT-PCR).Results provide a frame of gene expression changes in different development proglottids of ***,which may be helpful in understanding the pathogenesis of the worm and studying its genes concerned with reproductive organs development as a model organism.

关键词： cDNA Microarray Genes Expression Profiling Moniezia expansa

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PCR tools for the verification of the specific identity of tapeworm from dogs

PCR tools for the verification of the specific identity of t...

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中国畜牧兽医学会家畜寄生虫学分会第六次代表大会暨第十次学术研讨会

作者： R.S.Dai~(1,2),G.H.Liu~(1,2),R.Q.Lin~1,H.Q.Song~1,W.Liu~2,Y.Liu~(2*),X.Q.Zhu~(1**) (1.College of Veterinary Medicine,South China Agricultural University,Guangzhou,Guangdong province 510642, PR China 2.College of Veterinary Medicine,Hunan Agricultural University,Changsha 410128,Hunan province,PR China)

Based on the sequences of the internal transcribed spacers(ITS-1 and ITS-2) and mitochondrial (cox1) of DNA(rDNA) of tapeworm samples,specific forward primers were designed in the ITS-1 and cox1 for each of the tapeworm samples of *** primers were used individually together with a conserved primer in the large subunit of rDNA to amplify partial ITS-1 and/or ITS-2 of rDNA from 29 DNA samples from tapeworm samples from *** approach allowed their specific identification,with no amplicons being amplified from heterogeneous DNA samples,and sequencing confirmed the identity of the sequences *** PCR assays should provide useful tools for the diagnosis and molecular epidemiological investigations of tapeworm.

关键词： Internal transcribed spacers(ITS) Polymerase chain reaction(PCR) tapeworm dog

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Immunoproteomic analysis of the second-generation merozoite proteins of Eimeria tenella

Immunoproteomic analysis of the second-generation merozoite ...

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中国畜牧兽医学会家畜寄生虫学分会第六次代表大会暨第十次学术研讨会

作者： Liheng Liu,Ruofeng Yan,Lixin Xu,Fengbin Yan,Xiaokai Song,Xiangrui Li~* (College of Veterinary Medicine,Nanjing Agricultural University,Nanjing,Jiangsu 210095,PR China)

Whole proteins of the second-generation merozoite of Eimeria tenella were analyzed by twodimensional gel electrophoresis（2-DE） and western blot using the chicken sera infected artificially with ***. Approximately 640 spots were detected on proteome map of the second-generation merozoite stained by Coomassie brilliant blue G-250 and 85 spots were recognized on western blot map as *** four spots of the antigens were identified by matrix-assisted laser desorption ionization time-of-flight MS（MALDITOF-MS） and MALDI-TOFTOF -*** six proteins of *** and three homologous proteins to other apicomplexan parasites or protozoan were identified using 'Mascot' *** proteins included lactate dehydrogenase,enolase,14-3-3 protein, microneme proteins,tubulin beta chain,SERPIN1 protein precursor,large subunit ribosomal protein L23 and surface antigens of ***,heat shock protein（HSP70） of Eimeria acervulina,protein phosphatase type 1 of Toxoplasma gondii and hypothetical protein GSPATT00020155001 of Paramecium *** rest proteins were searched against the *** protein sequence database using 'Mascot in-house'（version 2.1） search engine in automated mode and 11 unknown proteins were *** the amino acid sequence of the unknown proteins were searched using BLAST against nonredundant sequence databases（NCBI）,surface antigen 12 of *** and six homologous proteins to other apicomplexa parasites were *** were membrane skeleton protein IMC2A,mitochondrial F1- ATP synthase beta subunit precursor,3-oxoacyl-acylcarrier-protein synthase and catalase of ***,Vps26 of Plasmodium falciparum 3D7,and hypothetical protein TRIADDRAFT0424 of Trichoplax *** homolognes of 8 spots of the 44 analyzed proteins were *** proteins enriched the data of immunogenic proteins of the second-generation merozoite of ***.

关键词： Eimeria tenella Second-generation merozoite Immunoproteome

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The optimal immunization procedure of DNA vaccine pcDNA-TA4-IL-2 of Eimeria tenella and its cross-immunity to Eimeria necatrix and Eimeria acervulina

The optimal immunization procedure of DNA vaccine pcDNA-TA4-...

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中国畜牧兽医学会家畜寄生虫学分会第六次代表大会暨第十次学术研讨会

作者： Song-Xiaokai,Xu-Lixin,Yan-Ruofeng,Xu-Qianming,Huang-Xinmei,Li-Xiangrui~* (College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,PR China)

Fusion DNAvaccines co-expressed Eimeria tenella TA4 and chicken IL-2(chIL-2) were constructed and their efficacy against *** challenge were *** immunization procedure of DNA vaccine pcDNA-TA4-IL-2 of Eimeria tenella,including route,dose,time of immunization and age of primary immunization of chicken,was optimized and the stability and the cross-species protection of the vaccine were also *** of immunization was evaluated on the basis of oocyst decrease ratio,lesion score,body-weight gain and the anti-coccidial index(ACI).Results indicated DNA vaccines pcDNA3.1-TA4-IL-2,pcDNA3.1- TA4 and pcDNA4.0c-IL-2 were successfully constructed and the antigen genes could be expressed effectively in *** animal experimental results showed that DNA vaccines could obviously alleviate cecal lesions, body weight loss and increase oocyst decrease *** ACI of pcDNA3.0-TA4-IL-2 group was 192,higher than that of pcDNA3.1-TA4 *** optimization of immunization procedure of DNA vaccine pcDNA-TA4- IL-2 showed that 25μg was the optimal dose and intramuscular injection was the most effective route to induce protective *** were no significant differences of ACIs between boosting and non-boosting *** time and temperature had little effect on the immunizing efficacy of the *** vaccine could provide partial cross-protection against the challenge with *** and ***,but not with E. maxima.

关键词： E.tenella TA4 DNA vaccine chIL-2 immunization procedure cross-protection

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