The study describes cross protection experiments with chimeric DNA vaccine pVAX1-cSZ2-IL-2 to determine its efficacy against four important Eimeria ***-day-old chickens were randomly divided into nine groups;group 1 n...
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The study describes cross protection experiments with chimeric DNA vaccine pVAX1-cSZ2-IL-2 to determine its efficacy against four important Eimeria ***-day-old chickens were randomly divided into nine groups;group 1 negative control,groups 2,3,4,5 positive controls;and groups 6,7,8 and 9 experimental *** days 7 and 14,groups 1-5 were injected with TE buffer,and groups 6-9 with the *** 21 days of age,all chickens were inoculated with 5×104 sporulated oocysts except for the negative *** 2 and 6 were inoculated with Eimeria tenella, groups 3 and 7 with Eimeria necatrix,groups 4 and 8 with Eimeria acervulina and groups 5 and 9 with Eimeria *** days later,all chickens were weighed and slaughtered to obtain intestinal samples. Efficacy of immunization was evaluated on the basis of oocyst decrease ratio,lesion score,body-weight gain and anti-coccidial *** results indicated that the recombinant plasmid can induce host immune responses by alleviating intestinal lesions,body weight loss and oocysl ratio and imparting good protection against *** and ***,medium protection against *** but little effect against E. *** is concluded that the conserved antigen can provide cross protection and should be explored further.
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