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Anti-idiotype down-regulation of PRRSV vaccination against highly pathogenic PRRSV infection

Anti-idiotype down-regulation of PRRSV vaccination against h...

中国畜牧兽医学会畜牧兽医生物技术学分会暨中国免疫学会兽医免疫分会第八次学术研讨会

作者： ZHOU En-Min~(1*),WANG Gang~(1,2),SONG Teng-fei~(1,2),YU Ying~1,CAI Xue-hui~2,LIU Yong-gang~2,SHI Wen-da~2,RONG Fu-long~2,DONG Jian-guo~2,LIU He~2,WU Jia-nan~2 (1.College of Veterinary Medicine,Department of Preventive Veterinary Medicine,Shandong Agricultural University,Taian,271018,China 2. Key Laboratory of State Veterinary Biotechnology,Harbin Veterinary Research Institute, Chinese Academy of Agricultureal Science,Harbin 150001,China)

Our previous studies demonstrated that pigs infected with PRRSV produced two sets of auto-anti-idiotypic antibodies (auto-Ab2s) against idiotypic antibodies to M and Gp5 protein(1,2).Pigs produced auto-Ab2s at early stage of infection(35 days post infection,DPI) were identified as non-virus carriers,whereas that had auto-Ab2s at later stage of infection(77 DPI) became virus

关键词： PRRSV 生物技术学 DPC Anti-idiotype down-regulation of PRRSV vaccination against highly pathogenic PRRSV infection

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Identification of putative virulent genes by SSH in Streptococcus equi ssp. zooepidemicus

Identification of putative virulent genes by SSH in Streptoc...

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中国畜牧兽医学会畜牧兽医生物技术学分会暨中国免疫学会兽医免疫分会第八次学术研讨会

作者： MA Zhe,FAN Hong-jie~*,LU Cheng-ping (College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095)

Suppression subtractive hybridization(SSH) was performed with virulent strain ATCC35246 and avirulent strain ST171 to identify novel genes associated with virulence in Streptococcus equi ***(SEZ).There were fourteen genomic regions that were only present in virulent strain *** regions encoded 14 putative proteins,some of which were homologous to proteins associated with cellular surface structure,molecular synthesis,energy metabolism,regulation,transport systems and others of unknown *** for 6 particular regions were designed from the already published SEZ sequence. We then used PCR to evaluate the distribution and conservation of these 6 DNA fragments in various SEZ strains collected from different sources,regions,groups and *** results showed that these 6 DNA fragments were widely distributed in SEZ strains,yet they were not existence in the avirulent strain ***,these fragments could not be detected in other Streptococcus groups.

关键词： Streptococcus equi ssp zooepidemicus Suppression subtractive hybridization Putative virulent genes PCR detection

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Identification of CD8+ cytotoxic T lymphocyte epitopes from porcine reproductive and respiratory syndrome virus matrix protein in BALB/c mice

Identification of CD8+ cytotoxic T lymphocyte epitopes from ...

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中国畜牧兽医学会畜牧兽医生物技术学分会暨中国免疫学会兽医免疫分会第八次学术研讨会

作者： ZHANG Wei-jun~(1,3),LIN Yan~(1,3),BAI Yu~(1,3),TONG Tie-gang~(1,3),WANG Qun~(1,3),LIU Ni-hong~(1,3), LIU Guang-liang~(1,3),XIAO Yi-hong~(1,3),YANG Tao~(1,3),BU Zhi-gao~(1,3),TONG Guang-zhi~(1,3),WU Dong-lai~(1,3*) (1.The Key Laboratory of Veterinary Public Health,Ministry of Agriculture,State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China 2.Shanghai Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Shanghai 200241,China 3.Graduate School of Chinese Academy of Agricultural Sciences,Beijing 100081,China)

Twenty-seven nonapeptides derived from the matrix(M) protein of porcine reproductive and respiratory syndrome virus(PRRSV) were screened for their ability to elicit a recall interferon-γ(IFN-γ) response from the splenocytes of BALB/c mice following DNA vaccination and a booster vaccination with recombinant vaccinia virus rWR-PRRSV-M.

关键词： PRRSV Identification of CD8 cytotoxic T lymphocyte epitopes from porcine reproductive and respiratory syndrome virus matrix protein in BALB/c mice

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Expression,Purification and Activity Analysis of the N-terminal Domain of Lipoprotein LppQ of Mycoplasma Mycoide subsp. Mycoides SC

Expression,Purification and Activity Analysis of the N-termi...

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中国畜牧兽医学会畜牧兽医生物技术学分会暨中国免疫学会兽医免疫分会第八次学术研讨会

作者： XIN Jiu-qing~*,LI Yuan,GAO Yu-long,CHEN Chao,ZHANG Jian-hua,LIU Yang (State Contagious Bovine Pleuropneumonia Reference Laboratory, Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China)

The lipoprotein LppQ is specific to Mycoplasma mycoides *** SC（MmmSC） and was found in the type strain and in field strains isolated in Europe,Africa,and Australia,as well as in vaccinal *** N-terminal domain of LppQ possesses strong immunogenicity and induced a specific and persistent immune response in naturally and experimentally infected *** this study,the gene sequence coding the N-terminal domain of LppQ was amplified from MmmSC HVRI-X strain by PCR using special primers and mutated with a one-step overlap extension PCR method from a Mycoplasma-specific TGA（Trp） codon into a universal TGG（Trp） *** mutated gene was overexpressed in Escherichia coli and the soluble protein was purified with Ni-NTA His·Bind *** was optimized by varying the OD measurements of the cell cultures,IPTG concentration and the induction *** amount of recombinant protein reached 53.7%of the total mass of bacterial *** activity of the purified protein was examined with Western blot analysis and ELISA *** purified protein reacted strongly with the standard positive sera and didn't react with the standard negative sera of contagious bovine pleuropneumonia（CBPP）.These findings indicate LppQ may be a valuable antigen for development of specific and sensitive test methods for the control of this disease in cattle.

关键词： Mycoplasma mycoides subsp.mycoides SC Lipoprotein LppQ Expression

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学术会议征文通知

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中国预防兽医学报 2010年第7期32卷 581-581页

中国畜牧兽医学会畜牧兽医生物技术学分会暨中国免疫学会兽医免疫分会第八次学术研讨会,拟定于2010年8月24日至27日在哈尔滨举行,会议主题为"新发生及重新发生的动物传染病",现开始征集论文。

关键词：会议中国免疫学会

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Cloning,expression and immunocompetence analysis of gene encoding the glutamate dehydrogenase of Streptococcus suis serotype 2

Cloning,expression and immunocompetence analysis of gene enc...

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中国畜牧兽医学会畜牧兽医生物技术学分会暨中国免疫学会兽医免疫分会第八次学术研讨会

作者： YANG Wei-jun~(1,3*),YANG Zhi-biao~(1*),CAI Xue-hui~2,HAO Yong-qing~3,ZHENG Sheng-bo~1, LAN Dao-liang~1,GU Jiang-ping~1,XING Rui~1,YUAN Cong-li~1,CUI Li~1,HUA Xiu-guo~1 (1.Shanghai Key Laboratory of Veterinary Biotechnology,College of Agricultural and Biology,Shanghai Jiaotong University, Shanghai 200240,China 2.Division of Swine Infectious Diseases,State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences,Harbin 150001,China 3.College of Animal Science and Animal Medicine,Inner Mongolia Agricultural University,Huhhot 010018,China)

This study aimed to study the immune activity of glutamate dehydrogenase(GDH) of streptococcus suis serotype 2 and detect its ***:The GDH gene was cloned and *** GDH protein was expressed in a prokaryotic expression system and purified by affinity *** of the resulting protein was analyzed by western blot with anti-GDH monoclonal *** fusion protein was efficiently *** blot analysis confirmed that the resulting protein was *** GDH gene of *** 2 was successfully cloned and the high expressed,which facilitated the further studies on the development of immunological detection and vaccine design.

关键词： Streptococcus suis serotype 2 GDH prokaryotic expression Immunocompetence

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Inhibition of porcine Transmissible gastroenteritis virus (TGEV) replication in mini-pigs by shRNA

Inhibition of porcine Transmissible gastroenteritis virus (T...

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中国畜牧兽医学会畜牧兽医生物技术学分会暨中国免疫学会兽医免疫分会第八次学术研讨会

作者： ZHOU Jun-fang~(1,2),HUANG Fen~2,HUA Xiu-guo~(2*),CUI Lia,ZHANG Wen-na,SHENG Ya-na,YAN Yi-jia~1, CHEN Pi-ren~1,DING De-zhong~3,MOU Jing~1,CHEN Qi~4,LAN Dao-liang~1,YANG Zhi-biao~1 (1.Shanghai Key Laboratory of Veterinary Biotechnology,College of Agricultural and Biology,Shanghai Jiao Tong University, Shanghai 200240,China 2.East China Sea Fisheries Research Institute,Chinese Academy of Fisheries Science,Shanghai 200090,China 3.Tyco Clinical Institute,Shanghai 200233,China 4.Shanghai Animal Disease Control Center,Shanghai 201103,China)

Transmissible gastroenteritis virus(TGEV) is the causative agent of porcine Transmissible gastroenteritis(TGE), characterized by high mortality and severely retarded growth in piglets that dramatically affects the porcine ***,we have identified two shRNA-expressing plasmids pEGFP-U6/P1 and pEGFP-U6/P2 that target RNA-dependent RNA polymerase (RdRP) gene of TGEV with more than 95%of virus inhibition in *** this study,inhibition of the TGEV replication by pEGFP-U6/P1 and pEGFP-U6/P2 was tested in *** mini-pigs at 25 days old were injected with the shRNA-expressing plasmids and then infected with *** results from the analyses of clinical signs,histopathology,indirect immunofluorescence(IIF) and RT-PCR show that the two shRNA-expressing plasmids could significantly decrease the quantity of TGEV in different organs and protect mini-pigs from TGEV *** findings illustrate the prospect for TGEV-specific shRNAs to be new anti-TGEV agents.

关键词： TGEV shRNA mini-pig

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Engineering Foot-and-Mouth disease viruses with increased growth properties and antigenic match for the development of improved vaccine

Engineering Foot-and-Mouth disease viruses with increased gr...

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中国畜牧兽医学会畜牧兽医生物技术学分会暨中国免疫学会兽医免疫分会第八次学术研讨会

作者： ZHENG Hai-xue~*,GUO Jian-hong,JIN Ye,YANG Fan,HE Ji-jun,ZHANG Ke-san, LIU Xiang-tao~*,CAI Xue-peng (State Key Laboratory of Veterinary Etiological Biology,Key Laboratory of Animal Virology of Ministry of Agricultural, Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China)

A licensed vaccine is currently not available against serotype A foot-and-mouth disease(FMD) in China since A/WH/CHA/09 was isolated in 2009,partly because it does not replicate well in BHK *** this end,a novel plasmid-based reverse genetics system was employed to construct a chimeric strain by replacing the nearly complete P1 gene in the vaccine strain O/CHA/99 with that in the epidemic stain A/WH/CHA/*** chimeric vius displayed similar growth kinetics with its parental strains and was chosen as the vaccine candidate strain after 12 passages in BHK cells. Subsequently,immunization with the inactivated vaccine was conducted in cattle and humoral immune responses in most of vaccinated cattle were induced at day *** result of virus challenge by A/WH/CHA/09 at day 28 indicates that the group vaccinated by a 10μg dose was fully protected without sub-clinic *** together,our data demonstrate the chimeric vius with O/CHA/99 not only breaks through the bottleneck of propagation in BHK cells compared with A/WH/CHA/09,provides excellent antigenic matching against serotype A FMD,but also is also a potential marker vaccine to distinguish infection and vaccination,which suggests reverse genetics technology is a potential tool to engineer a vaccine candidate for FMD prevention and control.

关键词： Foot-and-mouth disease Vaccine Protection Reverse genetics

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微生物饲料添加剂益生康对肉仔鸡营养代谢与免疫功能的调控机理

微生物饲料添加剂益生康对肉仔鸡营养代谢与免疫功能的调控机理

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第三届第八次全国学术研讨会暨动物微生态企业发展战略论坛

作者：张日俊潘淑媛白永义王广秋汪淑云中国农业大学饲料生物技术实验室中国农业大学饲料生物技术实验室河北省承德畜牧局河北省承德畜牧局河北省承德畜牧局

本研究通过肉仔鸡饲养试验以研究益生康微生物饲料添加剂的最适用量以及对宿主营养代谢和免疫功能的调控机理．1000只肉仔鸡分为5个处理,在基础日粮中分别添加0,0．05％,0．1％,0．2％的益生康和黄霉素10mg／kg．在7和14日龄,在肉仔鸡... 详细信息

本研究通过肉仔鸡饲养试验以研究益生康微生物饲料添加剂的最适用量以及对宿主营养代谢和免疫功能的调控机理．1000只肉仔鸡分为5个处理,在基础日粮中分别添加0,0．05％,0．1％,0．2％的益生康和黄霉素10mg／kg．在7和14日龄,在肉仔鸡胫部注射0．5mL浓度为5g/L(W／V)的牛血清白蛋白 (BSA)抗原,检测腹脂率、生产性能、免疫学、微生物学、生物化学指标．试验表明:(1)肉仔鸡饲料中益生康的最佳添加量为0．1％,能能显著提高肉仔鸡生长、增重和饲料转化效率(P<0．05),效果优于添加黄霉素组;(2)YSK能显著刺激胸腺、脾脏和法氏囊的生长发育,增强鸡T或B淋巴细胞的活性和提高血清球蛋白的含量及抗体水平,从而提高机体的细胞免疫和体液免疫功能;(3)YSK能明显增加血清总蛋白、球蛋白含量,改善蛋白质代谢,并能提高血清脂肪酶活性,降低血清总胆固醇和甘油三酯含量调节脂肪代谢,显著降低腹脂率(P<0．05);(4)YSK可明显减少空肠和直肠中的大肠杆菌, 增加乳酸杆菌数量,调节菌群结构以及降低肠道的pH值(P<0．05,),酸化肠道环境,促进鸡的健康和减少鸡舍臭味;(5)YSK对营养代谢和免疫调控的机理为:YSK有益微生物通过分泌消化酶和酸化作用对消化酶的激活作用来增加营养素的吸收利用率,促进肉仔鸡生长,也通过其微生物产生的B 族维生素、有机酸和其它活性物质提高生产性能和生长速度;通过刺激免疫器官发育和提高抗体和淋巴细胞活力以及和优化肠道菌群结构(乳酸杆菌增加,大肠杆菌减少)提高肉仔鸡的健康状况。

关键词：微生物饲料添加剂益生康肉仔鸡细胞免疫体液免疫营养代谢调控机理

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