Whole proteins of the second-generation merozoite of Eimeria tenella were analyzed by twodimensional gel electrophoresis(2-DE) and western blot using the chicken sera infected artificially with ***. Approximately 64...
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Whole proteins of the second-generation merozoite of Eimeria tenella were analyzed by twodimensional gel electrophoresis(2-DE) and western blot using the chicken sera infected artificially with ***. Approximately 640 spots were detected on proteome map of the second-generation merozoite stained by Coomassie brilliant blue G-250 and 85 spots were recognized on western blot map as *** four spots of the antigens were identified by matrix-assisted laser desorption ionization time-of-flight MS(MALDITOF-MS) and MALDI-TOFTOF -*** six proteins of *** and three homologous proteins to other apicomplexan parasites or protozoan were identified using 'Mascot' *** proteins included lactate dehydrogenase,enolase,14-3-3 protein, microneme proteins,tubulin beta chain,SERPIN1 protein precursor,large subunit ribosomal protein L23 and surface antigens of ***,heat shock protein(HSP70) of Eimeria acervulina,protein phosphatase type 1 of Toxoplasma gondii and hypothetical protein GSPATT00020155001 of Paramecium *** rest proteins were searched against the *** protein sequence database using 'Mascot in-house'(version 2.1) search engine in automated mode and 11 unknown proteins were *** the amino acid sequence of the unknown proteins were searched using BLAST against nonredundant sequence databases(NCBI),surface antigen 12 of *** and six homologous proteins to other apicomplexa parasites were *** were membrane skeleton protein IMC2A,mitochondrial F1- ATP synthase beta subunit precursor,3-oxoacyl-acylcarrier-protein synthase and catalase of ***,Vps26 of Plasmodium falciparum 3D7,and hypothetical protein TRIADDRAFT0424 of Trichoplax *** homolognes of 8 spots of the 44 analyzed proteins were *** proteins enriched the data of immunogenic proteins of the second-generation merozoite of ***.
Fusion DNAvaccines co-expressed Eimeria tenella TA4 and chicken IL-2(chIL-2) were constructed and their efficacy against *** challenge were *** immunization procedure of DNA vaccine pcDNA-TA4-IL-2 of Eimeria tenella,i...
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Fusion DNAvaccines co-expressed Eimeria tenella TA4 and chicken IL-2(chIL-2) were constructed and their efficacy against *** challenge were *** immunization procedure of DNA vaccine pcDNA-TA4-IL-2 of Eimeria tenella,including route,dose,time of immunization and age of primary immunization of chicken,was optimized and the stability and the cross-species protection of the vaccine were also *** of immunization was evaluated on the basis of oocyst decrease ratio,lesion score,body-weight gain and the anti-coccidial index(ACI).Results indicated DNA vaccines pcDNA3.1-TA4-IL-2,pcDNA3.1- TA4 and pcDNA4.0c-IL-2 were successfully constructed and the antigen genes could be expressed effectively in *** animal experimental results showed that DNA vaccines could obviously alleviate cecal lesions, body weight loss and increase oocyst decrease *** ACI of pcDNA3.0-TA4-IL-2 group was 192,higher than that of pcDNA3.1-TA4 *** optimization of immunization procedure of DNA vaccine pcDNA-TA4- IL-2 showed that 25μg was the optimal dose and intramuscular injection was the most effective route to induce protective *** were no significant differences of ACIs between boosting and non-boosting *** time and temperature had little effect on the immunizing efficacy of the *** vaccine could provide partial cross-protection against the challenge with *** and ***,but not with E. maxima.
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