刚出壳雏鸡的发病和死亡,不仅直接影响孵化场和肉鸡养殖场的经济效益,同时这些病或死的雏鸡还可能传染健康雏鸡,给商品鸡场造成污染。随着养鸡规模的扩大,垂直传播病原菌数量的增加,病原菌成为刚出壳雏鸡发病的主要原因之一,因此,探究出壳肉鸡弱雏的病原菌感染情况,对有效防治该病的发生具有重要意义。本试验通过对10只出壳肉鸡弱雏进行解剖,从肝脏,心脏以及脾脏分离菌株,培养、纯化,革兰氏染色观察菌落形态,使用鉴别培养基(HE培养基、叠氮钠-结晶紫-七叶苷培养基、营养琼脂培养基和麦康凯培养基四种培养基)初步判断菌种;又通过DNA提取进行16S r RNA PCR扩增,扩增产物经1%琼脂糖凝胶电泳检测;测序结果在Gen Bank数据库中进行Blast分析比对,用DNAStar软件对相近序列进行同源性分析,确定菌种。10只雏鸡共有6只分离出病原菌,占60%。分离出21株病原菌,经形态学观察、鉴别培养基培养、鉴定检测、Blast分析比对,结果如下:大肠杆菌11株,占52.4%,肠球菌6株,占28.6%,奇异变形杆菌4株,占19.0%。导致出壳雏鸡病、死的主要病原菌是大肠杆菌、肠球菌和奇异变形杆菌。据了解,现在这三种细菌作为主要致病菌已经严重危害了养殖业的健康发展,所以研究以上细菌的快速检测方法对于防治由这三种细菌引起的疾病具有很重要意义。我们的调查结果,对导致出壳雏鸡病、死的主要病原菌有了新的发现,有利于出壳雏鸡成活率的提高。但是,试验所采集的弱雏较少,所分析的结果只具有一定的参考意义,为下一步的实验打下一个良好的基础,应通过更多的弱雏病原菌分离鉴定来使实验数据更为准确,也为防治疾病提供更多的依据。
Introduction Intestinal microflora exerts great influences on the host's *** gut diseases are closely related to the change of the microbial *** neonatal piglet diarrhea causes significant economic losses,the micr...
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Introduction Intestinal microflora exerts great influences on the host's *** gut diseases are closely related to the change of the microbial *** neonatal piglet diarrhea causes significant economic losses,the microbiome composition changes remain *** this study,our goals are to find the intestinal microbial composition differences between the healthy and diarrheal neonatal piglets,even the
Introduction Salmonella was one of the most important food-borne pathogens worldwide and caused tremendous economic losses in poultry *** was estimated that approximately 70%-80%of food-borne bacterial outbreaks were ...
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Introduction Salmonella was one of the most important food-borne pathogens worldwide and caused tremendous economic losses in poultry *** was estimated that approximately 70%-80%of food-borne bacterial outbreaks were caused by Salmonella in *** were used to control Salmonella in the past decades,however,due to the emergence and of antimicrobial resistance,the use of probiotics
Introduction White shrimp Penaeus vannamei(also known as Litopenaeus vannamei) is the primary penaeid shrimp species currently being cultured in Central and South America,as well as Pacific Rim ***,the diseases such a...
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Introduction White shrimp Penaeus vannamei(also known as Litopenaeus vannamei) is the primary penaeid shrimp species currently being cultured in Central and South America,as well as Pacific Rim ***,the diseases such as Taura syndrome virus(TSV) and white spot syndrome virus(WSSV)often occur and result in serious economic *** recent decades,disease prevention and control have
Introduction The crp gene,conserved in diverse bacterial species,encodes the cyclic AMP receptor protein(CRP),a global regulator that modulates the expression of a number of genes required for virulence,carbohydrate m...
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Introduction The crp gene,conserved in diverse bacterial species,encodes the cyclic AMP receptor protein(CRP),a global regulator that modulates the expression of a number of genes required for virulence,carbohydrate metabolism,flagellum synthesis,and glycogen *** studies has been constructed crp deletion mutants of *** SL1344 through a double-crossover event using the suicide vector pRE112,and the mutant
Introduction China is the largest antibiotics producer and consumer in the world and large amount of antibiotics were applied in livestock ***,the use of antibiotics in feed is poorly *** are live microorganisms that,...
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Introduction China is the largest antibiotics producer and consumer in the world and large amount of antibiotics were applied in livestock ***,the use of antibiotics in feed is poorly *** are live microorganisms that,when administrated in adequate amounts,confer a health benefit on the *** studies showed that probiotics have positive effects on pig health,including
Introduction The giant panda,Ailuropoda melanoleuca,is famous as a living *** is a vulnerable,endemic species that is extremely popular *** strategies have resulted in the survival of about 1800 giant pandas *** giant...
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Introduction The giant panda,Ailuropoda melanoleuca,is famous as a living *** is a vulnerable,endemic species that is extremely popular *** strategies have resulted in the survival of about 1800 giant pandas *** giant panda is an herbivore that has retained a typical carnivorous digestive *** is easily afflicted by various intestinal diseases when the feed structure
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