The apoptosis of granulosa cells is the main cause of follicular atresia, and endoplasmic reticulum (ER)stress is involved in the apoptosis of granulosa *** inducing factor (AIF) mediates caspase-independent apoptosis...
The apoptosis of granulosa cells is the main cause of follicular atresia, and endoplasmic reticulum (ER)stress is involved in the apoptosis of granulosa *** inducing factor (AIF) mediates caspase-independent apoptosis and causes chromatin condensation and DNA fragmentation, but its role in ER stress-mediated granulosa cell apoptosis during goat follicular atresia remains largely *** aim of this study was to investigate the function of AIF in the apoptosis of goat granulosa cells media13ted by ER *** results of immunohistochemical and Western blot analyses demonstrated that AIF was mainly located in granulosa cells, and the expression of AIF significantly increased during follicular ***, AIF-shRNA recombinant lentiviral vectors were constructed successfully and transfected into hTERT-goat granulosa cells (hTERT-GGCs).Real-time quantitative PCR and Western blot analysis confirmed that AIF was effectively knocked down in *** cytometry results showed that the knock-down of AIF in hTERT-GGCs reduced apoptosis due to serum starvation or thapsigargin (Tg)***, AIF depletion changed the expression of related molecular markers molecules of ER stress under Tg *** conclusion, AIF may serve as a key factor during follicular atresia and AIF depletion protects ER stress-mediated goat granulosa cell apoptosis.
We firstly found the expression of E-cadherin and Occludinin ovine oviduct and the expression was different in oestrous *** used immunohistochemistry method to examin the ellular localization of E-cadherin and Occludi...
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We firstly found the expression of E-cadherin and Occludinin ovine oviduct and the expression was different in oestrous *** used immunohistochemistry method to examin the ellular localization of E-cadherin and Occludin in ovine *** results showed that E-cadherin and Occludin was specifically expressed in the epithelial cells but not in the stromal *** we used westen blot method to examin the expression of E-cadherin and Occludin of ovine oviduct in the different time during the *** results showed that during the oestrous period the expression of both E-cadherin and Occludin were significantly upregulate than diestrum ***,we assumed that maybe estradiol and progesterone were the reason to enhance higher expression of E-cadherin and Occludin in ovine oviduct epithelial *** we treated the cultured ovine oviduct epithelial cells respectively with exogenous estradiol(10 g/ml、10g/ml、10g/ml、10g/ml、10g/ml) and progesterone(10mol/l、10mol/l、 10mol/l、 10mol/l、10mol/l) and harvested at different time（0h、3h、6h、12h、24h、48h）.Both the westen blot and qRT-PCR analysis showed that estradiol and progesterone could significantly upregulate the expression of E-cadherin and Occludin in a concentration-and time-dependent ***,it was found that the expression of E-cadherin and Occludin reached the maximum levle when treated with 10g/ml estradiol and 10mol/l progesterone at 24 h.
Leydig cells play an important role in mammalian reproductive physiology and endocrine, the secretion of reproductive hormones are controlled by clock ***1d1(Rev-erbα), one of the clock genes, is a transcription fact...
Leydig cells play an important role in mammalian reproductive physiology and endocrine, the secretion of reproductive hormones are controlled by clock ***1d1(Rev-erbα), one of the clock genes, is a transcription factor that plays central roles in the regulation of circadian *** is still unkown that whether these clock genes exhibit a rhythmic expression and regulate testosterone production in mouse leydig *** this report, studies were carried out to investigate the rhythmic expression pattem of some clock genes at indicated time points and the relationship between Nr1d1 and *** leydig tumor cells(MLTC1) and primary mouse ledyig cells(MLC) were synchronized with dexamethasone(DXM), and cells were collected every 4h interval from the beginning of 12h after the ***-time fluorescence quantitative PCR (RT-qPCR) was used to detect the rhythmic expression pattern of clock genes like Bmal1(Brain and muscle ARNT-like protein 1)、 Nr1d1 、 Per1(Period1) and Dbp(D site of albumin promoter binding protein).Rhythmic expression of testosterone secretion genes in MLC were also measured by *** lentivirus vector pCD513B-U6 encoding Nr1 d 1 shRNA and nonsilencing negative control (shRNA-N) were constructed in our laboratory (Chen et al., 2016).
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