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养犬 2015年第1期26卷 46-46页

为进一步提高我国养犬科技水平,促进养犬科技事业的发展,中国畜牧兽医学会养犬学分会将于2015年5月中下旬举办"第十六次全国养犬学术研讨会"(会议通知另发)。本次会议将邀请著名专家、学者做精彩学术报告,并评选优秀论文。现... 详细信息

为进一步提高我国养犬科技水平,促进养犬科技事业的发展,中国畜牧兽医学会养犬学分会将于2015年5月中下旬举办"第十六次全国养犬学术研讨会"(会议通知另发)。本次会议将邀请著名专家、学者做精彩学术报告,并评选优秀论文。现将征文有关事宜通知如下:一、征文范围从事犬(宠物)的社会管理、训练、使用、繁殖、饲养、培育、疾病防治以及与犬有关的科研、教学、生产等单位和个人,均可投稿,会议将择优录用,编入论文集。

关键词：来稿格式北京万方社会管理引文出处疾病防治作者简介尾注通讯地址征文截止日期字符数

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Expression and localization of nerve growth factor(NGF)in testis of alpaca(Ilama pacos)

Expression and localization of nerve growth factor(NGF)in te...

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中国畜牧兽医学会动物解剖学及组织胚胎学分会第五次会员代表大会暨第十六次学术研讨会

作者： Haidong Wang~1,Yanjun Dong~1,Wenhua Chen~(1,2),Junping Hei~1,Changsheng Dong~1 1 College of Animal Science and Veterinary Medicine,Shanxi Agricultural University,China 2 Hanzhong people's hospital,China

During alpaca testis development and spermatogenesis,nerve growth factor(NGF) may be play an important *** main aim of this study is to determine the expression and localization of NGF in alpaca testis,and to discuss the important role of NGF in alpaca reproductive *** staining technique and real time PCR were *** expression of NGF in the same cells in 1-month alpacas (newborn alpacas) and 12-month alpacas,24-month alpacas showed significant differences(P<0.05);12- and 24-months alpaca showed no significant differences(P>0.05);NGF at different cell stages showed no significant differences(P>0.05).It suggests that NGF may be involved in the regulation of spermatogenesis, which provides direct evidence for NGF action in the alpaca testis during postnatal development and spermatogenesis.

关键词： alpaca testis NGF(nerve growth factor) SABC

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鲤鱼蛋白酶体激活因子PA28-β亚单位基因克隆、鉴定及时空转录分析

鲤鱼蛋白酶体激活因子PA28-β亚单位基因克隆、鉴定及时空转录分析

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中国畜牧兽医学会兽医病理学分会第十六次学术研讨会、中国病理生理学会动物病理生理专业委员会第十五次学术研讨会

作者：付保忠卢强王雯慧谭业平孙晓义李伟甘肃农业大学动物医学院

PA28-α和PA28-β被IFN-γ诱导后,可以激活20S蛋白酶体前体,使之产生抗原肽被MHCⅠ类分子识别呈递给细胞毒性T细胞,因此在MHCⅠ类抗原呈递中具有重要作用。编码PA28-α和PA28-β的基因PMSE1和PMSE2已在哺乳动物中被很好的鉴定和阐述,而... 详细信息

PA28-α和PA28-β被IFN-γ诱导后,可以激活20S蛋白酶体前体,使之产生抗原肽被MHCⅠ类分子识别呈递给细胞毒性T细胞,因此在MHCⅠ类抗原呈递中具有重要作用。编码PA28-α和PA28-β的基因PMSE1和PMSE2已在哺乳动物中被很好的鉴定和阐述,而在鱼类方面报道很少。本研究以荧光mRNA差异显示技术获得的含有鲤鱼PA28-β亚单位cDNA3′端部分片段为核酸探针,地高辛标记后,应用核酸杂交法筛选有丝分裂原刺激的鲤鱼外周血白细胞cDNA文库。此外,本研究根据PA28-β亚单位cDNA设计引物,应用半定量RT-PCR方法,分析了在分离培养的正常白细胞和丝列原刺激的白细胞中该亚单位基因不同时间的转录差异情况,还对PA28-β在正常鲤鱼和嗜水气单胞菌Ahcs01刺激后鲤鱼的组织转录情况进行了分析。结果显示:我们获得的鲤鱼PA28-β亚单位cDNA克隆全长1175bp,GenBank获得注册号为EU255233,含有5′非翻译区和3′非翻译区及3′poly(A)加尾信号(AATAAA)和poly(A)尾巴,中间是732bp大小编码244个氨基酸的开放阅读框。推测的蛋白序列与斑马鱼、石首鱼、比目鱼、河豚、短尾猊、狗、猕猴、牛、人和非洲蟾蜍的PA28-β同源性分别为95%、78%、73%、70%、65%、63%、63%、63%、63%和55%,在相同的进化距离下比较哺乳动物和鱼类,人、猩猩、猪、狗等哺乳动物保守序列比例约占70%,而鱼类则相对较低,与哺乳动物比较约只有30%保守,说明鱼类的分子进化要比哺乳动物快,鲤鱼与石首鱼、比目鱼、河豚相比,和斑马鱼的亲缘关系更大,这可能是鲤鱼和斑马鱼同属淡水鱼而石首鱼、比目鱼和河豚属于海水鱼的原因了。蛋白质序列比对显示鲤鱼PA28-β蛋白序列中同样含有KEKE基序、内质网膜保留信号KPSM、典型的富含脯氨酸基序、可能的蛋白激酶C识别位点、高度保守的激活环和高度同源的C末端区域5个哺乳动物中存在的保守功能区域;时间转录分析显示:丝裂原刺激白细胞中PA28-β转录增强,尤其是ConA刺激后增强尤为显著,但是随着时间的延长增强幅度有下降趋势,提示PA28-β的转录对于不同的诱导物敏感程度不同,也说明PA28-β可能在细胞受刺激早期发挥免疫作用;组织转录谱分析显示:在鲤鱼鳃、肾、脾脏、肌肉、脑、心、肝七个组织均有转录,嗜水气单胞菌Ahcs01菌株刺激鲤鱼48小时后转录不同程度增强,其中与免疫相关的组织增强明显,脾脏尤为显著,这与PA28-β在MHCⅠ类分子介导的抗原呈递过程中具有重要作用相一致。

关键词：鲤鱼 PA28-β cDNA克隆序列分析转录分析

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The Retino-vLGN Visual Pathway via the Tectum

The Retino-vLGN Visual Pathway via the Tectum

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中国畜牧兽医学会动物解剖学及组织胚胎学分会第五次会员代表大会暨第十六次学术研讨会

作者： Department of Animal Sciences Faculty of Life Sci.and Environment Teikyo University of Science Jumpei Naito

It has been in circulation from old time that birds are most visually dependent in all the vertebrates and they have prominent visual ***,some of visual abilities such as acute discrimination,color vision,motor vision,motor vision,and visual memory are comparable,or in some aspects,even superior,to those of mammals including humans[Hodos et al.,1985,Emmerton and Delius, 1980].Karten and his colleagues have vigorously and extensively done many variable works concerning avian visual ***,morphological analysis of the visual pathway to the ventral lateral geniculate nucleus(vLGN) has *** information from the retina is transmitted to the forebrain via two parallel pathways,the tectofugal and thalamofugal pathways in *** avian tectum is consisted of many cellular and fibrous laminas and the subclasses of RGCs project to the tectum with tectal lamina specificity(Yamagata and Sanes,1995a,b).Avian visual pathway to the vLGN composes a part of the tectofugal pathway and it is involved into color vision,papillary reflex,and kineoptic functions (Pateromiechelakis,1979,Maturana and Varela,1982,Gamlin et al.,1984,Gioanni et al.,1991).

关键词： The Retino-vLGN Visual Pathway via the Tectum Visual

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约氏乳杆菌L531通过IRP2途径调控铁代谢和氧化应激进而改善鼠伤寒沙门氏菌性腹泻

约氏乳杆菌L531通过IRP2途径调控铁代谢和氧化应激进而改善鼠伤寒...

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中国畜牧兽医学会兽医产科学分会第十六次学术研讨会

作者：陈柯源王九峰郭亮王静杨澜扈婷赵以晴王雪朱要宏中国农业大学动物医学院中国农业大学三亚研究院

引言鼠伤寒沙门氏菌,在世界范围内引起人和动物胃肠道疾病和全身炎症反应。其中,断奶仔猪腹泻作为最典型的临床症状之一,已成为动物源性食品卫生安全领域关注的焦点。沙门菌已经进化出逃避宿主营养免疫的机制,可利用宿主体内的铁促进细...

引言鼠伤寒沙门氏菌,在世界范围内引起人和动物胃肠道疾病和全身炎症反应。其中,断奶仔猪腹泻作为最典型的临床症状之一,已成为动物源性食品卫生安全领域关注的焦点。沙门菌已经进化出逃避宿主营养免疫的机制,可利用宿主体内的铁促进细菌生长。然而,鼠伤寒沙门氏菌诱导铁稳态失调的详细机制以及约氏乳杆菌L531是否能缓解鼠伤寒沙门氏菌引起的铁代谢紊乱也尚未阐明。基于此,本试验通过构建沙门菌感染IPEC-J2、Hela体外细胞模型和体内小鼠模型,探究并分析约氏乳杆菌L531预处理前后铁代谢和氧化应激水平变化,为约氏乳杆菌L531作为抗沙门菌感染的绿色饲料添加剂提供理论参考。

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Morphological Analysis of the African Ostrich Tibia

Morphological Analysis of the African Ostrich Tibia

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中国畜牧兽医学会动物解剖学及组织胚胎学分会第五次会员代表大会暨第十六次学术研讨会

作者： CHENG Jia-yue~(1,2),PENG Ke-mei~(1*),WANG Yun~1,LIU~1,Hua-zhenSONG Hui~1, TANG Zhong-lin~2,YANG Shu-lin~2 1 College of Animal Science and Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070 2 Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193

To reveal their morphological characteristics,the tibias of four ostriches(one-year-old) were examined in three aspects:bone mineral density(BMD),ultrastructure and *** results indicate that the ostrich tibia has some dramatic *** ostrich fibula shows degeneration and gradual concrescence with the *** addition,the ostrich ankle,the distal end of tibia,cannot *** BMD of the ostrich tibia was also found to be very high,about 4-8-fold greater than human and scanning electron microscopy indicated a firm,strong and non-honeycomb-shaped *** further observed three characteristics of the microstructure:(1) the layers of cortical bone are very clear;(2) the layers of the outer circumferential lamella and inner circumferential lamella are much thicker than those in other animals;and(3) there is a special"transition zone"between the outer circumferential lamella and osteon *** analysis has also revealed that the decalcification of ostrich bone requires more time than other species reported to date.

关键词： ostrich tibia osteon decalcification transition zone

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Nitric Oxide Enhances the Sensitivity of Alpaca Melanocytes to Respond to a-melanocyte stimulating hormone by Up-regulating Melanocortin-1 Receptor

Nitric Oxide Enhances the Sensitivity of Alpaca Melanocytes ...

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中国畜牧兽医学会动物解剖学及组织胚胎学分会第五次会员代表大会暨第十六次学术研讨会

作者： DONG Yan-jun,CAO Jing,WANG Hai-dong,ZHANG Jie,ZHU Zhi-wei,BAI Rui,HAO Huan-qing,HE Xiao-yan,Fan Rui wen,DONG Chang-sheng~(**) College of Animal Science and Technology,Shanxi Agricultural University,030801,Taigu,Shanxi,China.

Nitric oxide（NO） and a-melanocyte stimulating hormone（a-MSH） have been correlated with the synthesis of *** NO-dependent signaling of cellular response to activate the hypothalamopituitary proopiomelanocortin system,thereby enhances the hypophysial secretion of a-MSH to stimulatea-MSH-receptor responsive *** this study we investigated whether an NO-induced pathway can enhance the ability of the melanocyte to respond to a-MSH on melanogenesis in alpaca skin melanocytes in *** is important for us to know how to enhance the coat color of *** set up three groups for experiments using the third passage number of alpaca melanocytes:the control cultures were allowed a total of 5 days growth;the UV group cultures like the control group but the melanocytes were then irradiated everyday（once） with 312 mJ/cm of UVB;the UV+L-NAME group is the same as group UV but has the addition of 300μM L-NAME（every 6 hours）.To determine the inhibited effect of NO produce,NO produces were *** determine the effect of the NO to the key protein and gene of a-MSH pathway on melanogenesis,the key gene and protein of the a-MSH pathway were measured by quantitative real-time PCR and western *** results provide exciting new evidence that NO can enhance a-MSH pathway in alpaca skin melanocytes by elevated *** we suggest that the NO pathway may more rapidly cause the synthesis of melanin in alpaca skin under UV,which at that time elevates the expression of MC1R and stimulates the keratinocytes to secrete a-MSH to enhance the a-MSH pathway on melanogenesis. This process will be of considerable interest in future studies.

关键词： Nitric oxide(NO) a-melanocyte stimulating hormone(a-MSH) Melanocortin-1 receptor(MC1R) Alpaca Melanocyte

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Expression pattern of NMS/NMU2R mRNA in the pig reproductive axis during the estrus cycle and the role of NMS on the reproductive axis in vitro

Expression pattern of NMS/NMU2R mRNA in the pig reproductive...

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中国畜牧兽医学会动物解剖学及组织胚胎学分会第五次会员代表大会暨第十六次学术研讨会

作者： Guihong Yang,Zhihai Lei~* College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,Jiangsu,P R China

Evidence has revealed that neuromedin S(NMS) and neuromedin U receptor type-2(NMU2R) mRNAs are expressed in the central nervous system and reproductive *** data indicated that variation of NMS and NMU2R was due to the phases of the adult rat hypothalamus estrus ***, the expression and function of NMS in the pig reproductive axis remains *** this study,16 virginal gilts were classified into four groups:proestrus,estrus,diestrus 1,and diestrus 2;the expression of NMS and NMU2R in the cyclic pig hypothalamus-pituitary-ovary axis was studied by reverse transcriptaion-polymerase chain reaction(RT-PCR),and the effect of NMS on the reproductive axis in vitro was detected by radioimmunoassay(RIA).The cloned pig NMS and NMU2R sequences were 82%and 90.2%identical to those of the corresponding human homologues,***-PCR showed that NMS and NMU2R mRNA expression in the hypothalamus and pituitary changed with the estrus cycle,i.e.,with the highest level in the proestrus group and the lowest in the estrus *** the ovary,NMS and NMU2R expression was highest in the diestrus 2 group and the lowest in the proestrus *** the in vitro study, different concentrations of NMS induced the release of gonadotropin-releasing hormone,luteinizing hormone,and estradiol at different levels of the reproductive *** together,the expression pattern of NMS during the estrus cycle and its role in reproductive hormones in vitro provide novel evidences of the potential roles of NMS in the regulation of pig reproduction.

关键词： NMS NMU2R RT-PCR Radioimmunoassay Reproductive axis

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延黄牛ALDH1A1基因的遗传变异及其对脂代谢的影响

延黄牛ALDH1A1基因的遗传变异及其对脂代谢的影响

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第十六次全国畜禽遗传标记学术研讨会

作者：胡忠昌赵玉民吴健秦立红金海国刘理想曹阳延边大学农学院延吉133002 吉林省农业科学院畜牧科学分院公主岭136100 吉林省农业科学院畜牧科学分院公主岭136100

为探索乙醛脱氢酶1A1(acetaldehyde dehydrogenase 1A1,ALDH1A1)的遗传变异对脂代谢的影响,本试验以18月龄99头延黄牛母牛为研究对象,提取牛耳组织DNA,并采集1日龄犊牛皮下脂肪组织进行分离前体脂肪细胞.采用PCR扩增产物Sanger测序的方... 详细信息

为探索乙醛脱氢酶1A1(acetaldehyde dehydrogenase 1A1,ALDH1A1)的遗传变异对脂代谢的影响,本试验以18月龄99头延黄牛母牛为研究对象,提取牛耳组织DNA,并采集1日龄犊牛皮下脂肪组织进行分离前体脂肪细胞.采用PCR扩增产物Sanger测序的方法检测99头延黄牛ALDH1A1基因的单核苷酸多态性(SNP),运用SPSS19.0软件分析ALDH1A1基因多态性与肉用性状的关联性.应用油红O染色和甘油三脂测定法检测延黄牛成熟脂肪细胞中甘油三脂及脂滴分泌情况,通过干扰和过表达ALDH1A1检测该基因在脂肪细胞中的调控作用.结果显示,仅在延黄牛ALDH1A1基因第4外显子处检测到T/G突变,且引起编码氨基酸的改变.关联分析结果表明;ALDH1A1基因第4外显子不同基因型与延黄牛体尺性状中的十字部高、胸围、腹围和官围存在显著相关(p＜0.05),与肉质性状中的背膘厚存在显著相关(p＜0.05),与肌内脂肪具有一定的相关性(P=0.056).在延黄牛前体脂肪细胞形成过程中ALDH1A1基因表达显著增加,此外,几种转录因子PPARγ,C/EBPα和LPL也显著表达.ALDH1A1的过表达和干扰RNA分别促进和抑制牛前体脂肪细胞分化过程中不同时程mRNA水平的表达,也显著增加和减少牛成熟脂肪细胞内脂质积累和甘油三脂的生成.ALDH1A1和成脂因子的蛋白水平与mRNA水平呈现类似的变化.综上所述,ALDH1A1基因在延黄牛群体中存在多态性,且与延黄牛体尺和部分肉质性状存在显著性差异.在细胞学水平研究证实ALDH1A1在延黄牛前体脂肪细胞分化过程中发挥着重要的调控作用.

关键词：延黄牛 ALDH1A1基因单核苷酸多态性遗传变异脂代谢

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