Nitric oxide（NO） and a-melanocyte stimulating hormone（a-MSH） have been correlated with the synthesis of *** NO-dependent signaling of cellular response to activate the hypothalamopituitary proopiomelanocortin syst...
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Nitric oxide（NO） and a-melanocyte stimulating hormone（a-MSH） have been correlated with the synthesis of *** NO-dependent signaling of cellular response to activate the hypothalamopituitary proopiomelanocortin system,thereby enhances the hypophysial secretion of a-MSH to stimulatea-MSH-receptor responsive *** this study we investigated whether an NO-induced pathway can enhance the ability of the melanocyte to respond to a-MSH on melanogenesis in alpaca skin melanocytes in *** is important for us to know how to enhance the coat color of *** set up three groups for experiments using the third passage number of alpaca melanocytes:the control cultures were allowed a total of 5 days growth;the UV group cultures like the control group but the melanocytes were then irradiated everyday（once） with 312 mJ/cm of UVB;the UV+L-NAME group is the same as group UV but has the addition of 300μM L-NAME（every 6 hours）.To determine the inhibited effect of NO produce,NO produces were *** determine the effect of the NO to the key protein and gene of a-MSH pathway on melanogenesis,the key gene and protein of the a-MSH pathway were measured by quantitative real-time PCR and western *** results provide exciting new evidence that NO can enhance a-MSH pathway in alpaca skin melanocytes by elevated *** we suggest that the NO pathway may more rapidly cause the synthesis of melanin in alpaca skin under UV,which at that time elevates the expression of MC1R and stimulates the keratinocytes to secrete a-MSH to enhance the a-MSH pathway on melanogenesis. This process will be of considerable interest in future studies.
Evidence has revealed that neuromedin S(NMS) and neuromedin U receptor type-2(NMU2R) mRNAs are expressed in the central nervous system and reproductive *** data indicated that variation of NMS and NMU2R was due to the...
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Evidence has revealed that neuromedin S(NMS) and neuromedin U receptor type-2(NMU2R) mRNAs are expressed in the central nervous system and reproductive *** data indicated that variation of NMS and NMU2R was due to the phases of the adult rat hypothalamus estrus ***, the expression and function of NMS in the pig reproductive axis remains *** this study,16 virginal gilts were classified into four groups:proestrus,estrus,diestrus 1,and diestrus 2;the expression of NMS and NMU2R in the cyclic pig hypothalamus-pituitary-ovary axis was studied by reverse transcriptaion-polymerase chain reaction(RT-PCR),and the effect of NMS on the reproductive axis in vitro was detected by radioimmunoassay(RIA).The cloned pig NMS and NMU2R sequences were 82%and 90.2%identical to those of the corresponding human homologues,***-PCR showed that NMS and NMU2R mRNA expression in the hypothalamus and pituitary changed with the estrus cycle,i.e.,with the highest level in the proestrus group and the lowest in the estrus *** the ovary,NMS and NMU2R expression was highest in the diestrus 2 group and the lowest in the proestrus *** the in vitro study, different concentrations of NMS induced the release of gonadotropin-releasing hormone,luteinizing hormone,and estradiol at different levels of the reproductive *** together,the expression pattern of NMS during the estrus cycle and its role in reproductive hormones in vitro provide novel evidences of the potential roles of NMS in the regulation of pig reproduction.
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