The aim of this study is to investigate the effect of durations on nuclear maturation in vitro of feline oocytes. Following the in vitro maturation(IVM) in Medium 199 added with FSH and *** oocytes were treated for 28...
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The aim of this study is to investigate the effect of durations on nuclear maturation in vitro of feline oocytes. Following the in vitro maturation(IVM) in Medium 199 added with FSH and *** oocytes were treated for 28h.32h and 36h *** vitro maturated oocytes were subjected to artifical activation in 10%*** activated oocytes were cultured 6 *** rate of activated oocytes was evaluated on Day *** were stained with Hoechest 33342 on Day 6 to observe the potential of activated *** the same time,the matured oocytes were fixed in 3.7% paraformaldehyde,stained in FITC-LCA and *** observed the distribution of cortical granules(CGs) to evaluate the cytoplasmic *** cleave rate of activated oocytes in the 32h group was significantly higher than the 28h group (60.42%vs 43.16%.0.01
The protozoan parasite Toxoplasma gondii is the causative agent of a worldwide zoonosis and development of vaccine would be of great medical and veterinary *** the present study,two recombinant plasmids pcDNA /TgSAG1 ...
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The protozoan parasite Toxoplasma gondii is the causative agent of a worldwide zoonosis and development of vaccine would be of great medical and veterinary *** the present study,two recombinant plasmids pcDNA /TgSAG1 and pVAX/mIL-18 containing *** SAG1(TgSAG1) and murine IL-18 were evaluated for their ability to protect mice against *** *** were given 2 intramuscular immunizations 3 weeks apart,and challenged with *** 3 weeks *** animals vaccinated with pcDNA/TgSAG1 alone or with pVAX/mIL-18 developed specific anti-TLA(*** lysate antigen) ELISA antibody and T lymphocyte proliferation *** of pVAX/mIL-18 significantly increased the production of IFN-γand IL-2,indicating that IL-18 enhances the Th1-dominant immune *** experiments showed that co-injection of pVAX/mIL-18 significantly increased the survival rate(60%),compared with pcDNA/TgSAG1 alone providing partial protection(40%) against challenge(P<0.05).These results indicate that IL-18 may be an effective adjuvant for a toxoplasmosis vaccine.
Whole proteins of male and female adult Haemonchus contortus were analysed by immunoproteomic *** 662 and 680 spots were detected on proteome maps of male and female nematodes,respectively, stained with Coomassie bril...
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Whole proteins of male and female adult Haemonchus contortus were analysed by immunoproteomic *** 662 and 680 spots were detected on proteome maps of male and female nematodes,respectively, stained with Coomassie brilliant blue *** were 609 shared *** 193 and 196 spots were recognised on Western blot maps of male and female nematodes,respectively,using antiserum from naturally infected goats as the source of primary *** were 129 gender-specific spots in male nematodes and 132 in ***-three shared immunogenic spots were identified by MALDI-TOF or MALDI-TOF-TOF mass spectrometry. These proteins included glutamate dehydrogenase(GDH),homologues of dim-1,actin,globin-like excretory /secretory protein F6,glutathione S-transferase(GST),ATPase and glyceraldehyde-3-phosphate dehydrogenase. GDH and GST have been identified as immunogenic proteins of *** previously,whereas the other proteins are newly recognised immunogenic proteins in this nematode.
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