Whole proteins of the second-generation merozoite of Eimeria tenella were analyzed by twodimensional gel electrophoresis（2-DE） and western blot using the chicken sera infected artificially with ***. Approximately 64...
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Whole proteins of the second-generation merozoite of Eimeria tenella were analyzed by twodimensional gel electrophoresis（2-DE） and western blot using the chicken sera infected artificially with ***. Approximately 640 spots were detected on proteome map of the second-generation merozoite stained by Coomassie brilliant blue G-250 and 85 spots were recognized on western blot map as *** four spots of the antigens were identified by matrix-assisted laser desorption ionization time-of-flight MS（MALDITOF-MS） and MALDI-TOFTOF -*** six proteins of *** and three homologous proteins to other apicomplexan parasites or protozoan were identified using 'Mascot' *** proteins included lactate dehydrogenase,enolase,14-3-3 protein, microneme proteins,tubulin beta chain,SERPIN1 protein precursor,large subunit ribosomal protein L23 and surface antigens of ***,heat shock protein（HSP70） of Eimeria acervulina,protein phosphatase type 1 of Toxoplasma gondii and hypothetical protein GSPATT00020155001 of Paramecium *** rest proteins were searched against the *** protein sequence database using 'Mascot in-house'（version 2.1） search engine in automated mode and 11 unknown proteins were *** the amino acid sequence of the unknown proteins were searched using BLAST against nonredundant sequence databases（NCBI）,surface antigen 12 of *** and six homologous proteins to other apicomplexa parasites were *** were membrane skeleton protein IMC2A,mitochondrial F1- ATP synthase beta subunit precursor,3-oxoacyl-acylcarrier-protein synthase and catalase of ***,Vps26 of Plasmodium falciparum 3D7,and hypothetical protein TRIADDRAFT0424 of Trichoplax *** homolognes of 8 spots of the 44 analyzed proteins were *** proteins enriched the data of immunogenic proteins of the second-generation merozoite of ***.
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