Aims—To investigate in vitro property of probiotics to impair the adhesion of bacteria that usually associated with uterine infection in *** and Results—The adherence of several probiotics,i.e.,Enterococcus faecalis...
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Aims—To investigate in vitro property of probiotics to impair the adhesion of bacteria that usually associated with uterine infection in *** and Results—The adherence of several probiotics,i.e.,Enterococcus faecalis,Lactobacillus casei,Lactobacillus brevis,and Bacillus cereus,that isolated from cervical mucus in health cattle to hircine endometrial epithelial cell lines were investigated. The results indicate that all these strains exhibited different adhesion ***,the inhibitory effects of these probiotics organisms on the adherence of two endometritis-associated pathogens, Escherichia coli and Staphylococcus aureus,were determined.A significant decrease in the number of adhering pathogens was observed,using either preincubation,postincubation or coincubation of the pathogens with probiotics ***,although the level of adhesion of *** to endometrial epithelium was significantly higher than those of *** and ***,there was no significant difference of the adherence inhibition of two pathogenic bacteria to epithelial cells among three probiotics. Conclusion—These probiotics strains could be used as prevent colonization of the uterine tract by endometritis-associated *** and Impact of the Study—This is the first report of probiotics to inhibit the adhesion of endometritis-associated bacterial pathogens to endometrial epithelium, which shed light on the prevention and treatment of endometritis in cattle.
为了深入认识鸡舍环境微生物气溶胶的产生及其向舍外环境的传播,同时比较ERIC-PCR和PFGE对细菌的基因分型结果,本研究以大肠杆菌为指示菌,采用Andersen-6级空气微生物样品收集器,分别在两个鸡舍的舍内、舍外上风向10m,50m和下风向10m,50m,100m,200m,400m的距离处收集空气样品,同时采集鸡的粪便,分离、鉴定大肠杆菌。采用ERIC-PCR和PFGE两种方法对不同来源的大肠杆菌分离株进行基因分型,根据每个采样点大肠杆菌的相似性指数,确认动物舍内微生物气溶胶向舍外环境传播的模式,并比较这两种方法对大肠杆菌的基因分型结果,分析这两种分子生物学分型方法各自的优缺点和适应性。本实验共采集到28株大肠杆菌,ERIC-PCR和PFGE两种方法分别进行同源性鉴定显示,从鸡的粪便中分离到的大肠杆菌与从舍内空气中分离到的部分大肠杆菌(62.5%)具有相同来源;从鸡场舍外下风方向分离到的多数大肠杆菌(20%)与舍内空气或粪便中分离的大肠杆菌来源相同。而从鸡舍上风没有分离到大肠杆菌,排除了下风向采得的大肠杆菌来自上风向的可能。而很多从舍内空气和舍外下风方向分离到的大肠杆菌与粪便中的具有相同来源,说明粪便中的细菌能够形成气溶胶,并且通过舍内外气体交换传播到舍外,依气象条件传播到舍外不同的距离。造成周边环境的生物污染以及病原微生物的扩散。本研究同时对ERIC-PCR和P F G E两种方法所得的分型结果进行了比较。PFGE的分辨率高于ERIC-PCR,而且PFGE的重复性也很高,不同实验室得出的结果可以相互比较,是细菌基因分型的"金标准"。但是PFGE试验费用高,需要时间长,对试剂、仪器和操作都具有很高的要求;ERIC-PCR虽然分辨率不及PFGE,但是ERIC-PCR简单、快速,成本低,对操作和试剂以及仪器要求都不高,所以ERIC-PCR也可以对肠杆菌进行同源性鉴定,应用于流行病学调查和对气源性微生物传播的鉴定。由于ERIC-PCR和PFGE是根据DNA中不同的遗传特征进行分类的,所以这两种分型技术结合起来分析菌株的遗传进化关系,意义更大。
The present research evaluated the effects of probiotics,Lactobacillus acidophilus RS058, Rhodopseudomonas palustris GH642 and Bacillus coagulans NJ105,as water additives on growth performance and immune response of t...
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The present research evaluated the effects of probiotics,Lactobacillus acidophilus RS058, Rhodopseudomonas palustris GH642 and Bacillus coagulans NJ105,as water additives on growth performance and immune response of the white shrimp,Penaeus *** shrimp,with average weight of 3.38±0.13 g,were stocked in each of the 12 500-1 polypropylene tanks,with three tanks treated with *** RS058（T-1）,*** GH642（T-2） and *** NJ105（T-3）,respectively, at a final concentration of 1×10～7 cfu ml every day,and the remaining three tanks were without any probiotic and served as the *** trial was carried out for 35 days and the results indicated that probiotic treatments significantly improved the final weight,daily weight gain（DWG） and relative weight gain（RWG） of the shrimp（P<0.05） as compared with those of the *** were no significant differences（P>0.05） in final weight and RWG among the *** for T-1,no significant differences were observed in DWG and SOD activity compared with T-2 and ***, higher DWG（P<0.05） was observed in T-3 compared with T-2.A substaintial and significant increase （P<0.05） in phenoloxidase（PO） activity was found in T-3 compared with those of the other two probiotic *** activity of superoxide dismutase（SOD） was significantly higher for the shrimp in T-3 than that in *** were no significant differences（P>0.05） in the peroxidase（POD） activity and antibacterial activity among the shrimp treated with different probiotics.
Enterobacterial Repetitive Intergenic Consensus-PCR(ERIC-PCR) is a molecular biological technology that can be used to study microbial community diversity and *** many reports, investigations of microbial diversity fr...
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Enterobacterial Repetitive Intergenic Consensus-PCR(ERIC-PCR) is a molecular biological technology that can be used to study microbial community diversity and *** many reports, investigations of microbial diversity from environmental samples were based on the agarose gel electrophoresis(AGE) patterns of ERIC-PCR amplified *** is not a sound practice,since bands with identical positions can contain different sequences;thus,this practice could possibly exaggerate the similarities or diversities among *** mitigate this issue,we employed a denaturing gradient gel electrophoresis(DGGE) strategy to explore DNA bands with the same size, between ERIC-PCR profiles of *** software was used with Shannon-Wiener diversity index (H') to analyze ERIC-PCR fingerprint profiles.H' revealed that the microbial community diversity at DGGE was higher than with *** results of this study suggest that the ERIC-PCR assays with DGGE can provide a better assessment of electrophoresis pattern with regards to the structure of an intestinal microbial community.
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