Heme oxygenase（HO）is the enzyme that catalyzes the degradation of heme and leads to the formation of carbon monoxide,free iron and *** evidence suggests that HO-1,an inducible isoform of HO,can exert vaso-protective...
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Heme oxygenase（HO）is the enzyme that catalyzes the degradation of heme and leads to the formation of carbon monoxide,free iron and *** evidence suggests that HO-1,an inducible isoform of HO,can exert vaso-protective ***, such vascular benefit in diabetic vasculopathy remains largely *** present study investigates the impact of HO-1 induction on endothelial dysfunction in type 2 diabetic db/db *** db/db and non-diabetic db/mmice were treated with hemin
The development of nitroglycerine tolerance limits its clinical efficacy of nitric oxide(NO)*** cellular mechanisms have been proposed to explain this phenomenon,one of which is an exaggerated production of reactive o...
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The development of nitroglycerine tolerance limits its clinical efficacy of nitric oxide(NO)*** cellular mechanisms have been proposed to explain this phenomenon,one of which is an exaggerated production of reactive oxygen specious(ROS).The present study examined whether ROS contribute to the occurrence of nitroglycerine tolerance which is induced acutely in aortas isolated from male Sprague-Dawley *** aortas were exposed to 30μmol/L nitroglycerine(with and without pre-incubation of ROS inhibitors) for 90 min and washed out 4 times during the following 60 min before phenylephrine was added to induce a steady contraction. Subsequently,nitroglycerine was added cumulatively(3 nmol/L-50μmol/L)to evoke ***-induced relaxations were severely impaired after 90-min pre-exposure to *** with apocynin,tempol and tiron plus DETCA
Signal transducers and activators of transcription-3(STAT3),a central cytoplasmic transcription factor,is frequently overexpressed and constitutively activated during malignant *** overexpression of STAT3 in melanoma ...
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Signal transducers and activators of transcription-3(STAT3),a central cytoplasmic transcription factor,is frequently overexpressed and constitutively activated during malignant *** overexpression of STAT3 in melanoma cells is often observed and is suggested to be involved in tumorigenesis and *** this study,we designed several novel antisense RNA oligonucleotides targeting the STAT3 mRNA that was 2'-O-methyl modified with a 3'-butanol *** uniquely modified strategy dramatically increased the stability of the RNA *** results showed that the RNA oligonucleotides,namely STT-33 and STT- 34,strongly inhibited the target gene expression in the melanoma cells and induced an increase in the cell ***,the
The study was to investigate the effect of reducing central sympathetic nervous tone with clonidine on cardiomyocyte contraction, and further to analyze the influence of reducing the central sympathetic nervous tone o...
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The study was to investigate the effect of reducing central sympathetic nervous tone with clonidine on cardiomyocyte contraction, and further to analyze the influence of reducing the central sympathetic nervous tone on response ofβ andβ-AR in aged ventricular ***-Dawley rats were randomly divided into three groups:adult group,aged group and aged group with clonidine. Each group was pretreated with CGP20712A（CGP,a selectiveβ-AR antagonist）,ICI118,551（ICI,a selectiveβ-AR antagonist）, or non-pretreatment *** was followed by the observation of the response of cardiomyocytes to *** improved the contraction in aged group,yet did not reach the level in adult ***20712A reduced cardiomyocyte shortening amplitude in three groups,which was most clearly seen in adult ***118,551 had no effect on cardiomyocyte shortening ampli-
The latent membrane protein(LMP1 ) encoded by EBV is expressed in the majority of EBV-associated human malignancies, suggesting it is one of the major oncogenic factors in EBV-mediated *** the previous studies we expe...
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The latent membrane protein(LMP1 ) encoded by EBV is expressed in the majority of EBV-associated human malignancies, suggesting it is one of the major oncogenic factors in EBV-mediated *** the previous studies we experimentally demonstrated that the DNAzymes targeting LMP1 could specifically down-regulate the expression of LMP1,leading to an increased radiosensitivity both in cells and in a xenograft nasopharyngeal carcinoma(NPC) model in *** this study we explored the molecular mechanisms underlying the radiosensitization caused by the down-regulation of LMP1 in *** clinical samples,there was a positive correlation between the expression of LMP1 and *** cell models,it was confirmed that LMP1 could up-regulate the ATM expression. Bioinformatic analysis of the ATM promoter region revealed 3 tentative binding sites for NF-κ*** using a specific inhibitor of
Glial glutamate transporter-1（GLT-1）is the predominant subtype of glutamate transporters and is responsible for the clearance of extracellular glutamate and for limiting the concentration of extracellular *** previo...
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Glial glutamate transporter-1（GLT-1）is the predominant subtype of glutamate transporters and is responsible for the clearance of extracellular glutamate and for limiting the concentration of extracellular *** previous studies have shown that the upregulation of GLT-1 expression plays an important role in the induction of brain ischemic tolerance induced by cerebral ischemic preconditioning （CIP）.Whether the function of GLT-1 in the induction of brain ischemic tolerance would change or not remains to be ***,the present study was undertaken to observe changes in the binding properties including the amount and affinity ,and the glutamate uptake of GLT-1 in the process in rats by the radio ligand binding and uptake assay using L-[H]-glutamate. The results indicated that CIP for 3 min was able to increase the amount and affinity and the glutamate uptake of GLT-1 in hippocampal CA1 subfield either with or without the presence of the subsequent severe brain ischemic *** with the above changes,the CIP significantly reduced the delayed neuronal death in this region normally induced by lethal global cerebral *** is concluded that up-regulation in the amount and affinity and glutamate uptake of GLT-1 contributed to the neuronal protection of CIP against global cerebral ischemic insult.
Grifolin,a secondary metabolite isolated from the fresh fruiting bodies of mushroom Albatrellus confluens,inhibits the growth of some cancer cell lines in vitro by induction of apoptosis in the previous studies of our...
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Grifolin,a secondary metabolite isolated from the fresh fruiting bodies of mushroom Albatrellus confluens,inhibits the growth of some cancer cell lines in vitro by induction of apoptosis in the previous studies of our ***,the mechanisms are not completely *** expression profiling analysis of apoptosis-related gene provided a clue that death-associated protein kinase 1 (dapk1 ) gene was upregulated at least twofold in response to grifolin treatment in nasopharyngeal carcinoma cell ***,we further investigated the role of DAPK1 in apoptotic effect induced by grifolin in nasopharyngeal carcinoma *** observed that the expression of DAPK1 at mRNA and protein levels was induced by grifolin in a dose-dependent manner in nasopharyngeal carcinoma cell
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