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Neuroprotection of paclitaxel against cerebral ischemia/reperfusion induced brain injury through JNK3 signaling pathway

Neuroprotection of paclitaxel against cerebral ischemia/repe...

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Su-Hua QI~*,Xiao-Yu HOU,Juan-Juan GONG Research Center for Biochemistry and Molecular Biology and Provincial Key Laboratory of Brain Disease Bioinformation,Xuzhou Medical College,Xuzhou 221002,China

In this study,we investigated the neuroprotective effects of paclitaxel in transient cerebral ischemia and the possible regulatory mechanism underlying the *** data show that paclitaxel can down-regulate the increased MLK3,JNK3,c-Jun and Bcl-2 phosphorylation induced by ischemia *** violet staining and immunohistochemistry results demonstrated that paclitaxel had a neuroprotective effect against ischemia reperfusion-induced neuronal cell *** results indicate that paclitaxel has a neuroprotective role in ischemic injury through JNK3 signaling pathway and provides a novel possible drug in therapeutics of brain ischemia.

关键词： cerebral ischemia paclitaxel neuroprotection c-Jun N-terminal kinase 3

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Functional structure in the primary auditory cortex and auditory prosthesis

Functional structure in the primary auditory cortex and audi...

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Wen-Jie SONG Department of Sensory and Cognitive Physiology,Kumamoto University School of Medicine,1-1-1 Honjyo, Kumamoto,Kumamoto 860-8556,Japan

Topographic representation of sound frequency in the primary auditory cortex,or tonotopy,is widely *** real-time imaging with voltage-sensitive dyes,we have demonstrated dynamic activity propagation in an isofrequency *** activity can be induced by focal stimulation of the cortex,suggesting the anisotropic nature of the intrinsic circuit in the auditory cortex and the ability of such circuit in supporting activity propagation within an isofrequency *** with this notion,a significant portion of spontaneous events in the primary auditory cortex showed similar spatiotemporal pattern to tone-evoked *** test whether focal stimulation of the cortex can induce auditory perception,we trained animals in a trace-learning paradigm and tested the effect of cortical *** stimulation induced behavioral response conditioned with a tone after learning,but not after *** findings should have implications on auditory prosthesis by cortical stimulation.

关键词： Functional structure in the primary auditory cortex and auditory prosthesis

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α-Catulin CTN-1 is required for BK channel subcellular localization in ***

α-Catulin CTN-1 is required for BK channel subcellular loca...

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： William A Mohler Department of Neuroscience University of Connecticut Health CenterFarmingtonCTUSA

The BK channel,a voltage-and Ca-gated large-conductance potassium channel with many important functions,is often localized at specific subcellular *** proper subcellular localization is likely a prerequisite for the channel to perform its physiological functions,little is known about the molecular basis of ***,we show that CTN-1,a homologue of mammalian a-catulin,is required for subcellular localization of SLO-1,the Caenorhabditis elegans BK channel a-subunit,in body-wall muscle cells and ***-1 was identified in a genetic screen for mutants that suppressed a lethargic phenotype caused by expressing a gain-of-function (gf)isoform of ***-1 coclusters with SLO-1 at dense body regions of body-wall muscle cells and presynaptic sites of *** ctn-1 loss-of-function(lf)mutants,SLO-1 was mislocalized but its transcription and protein level were *** neurotransmitter release was increased at the neuromuscular junction in ctn-1(lf)mutant as well as slo-l(lf)*** results suggest that CTN-1 plays an important role in BK channel function by mediating channel subcellular localization.

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Maturation of “Neocortex Isol” in vivo

Maturation of “Neocortex Isol” in vivo

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Fadel Tissir Andr *** Institute of Neuroscience Universite catholique de Louvain

＜正＞How much neocortical development depends on connections remains ***,we show that Celsr3|D1x mutant mice have no extrinsic neocortical connections yet survive to postnatal day 20,acquire a basic behavioral repertoire,and display spontaneous hyperactivity,with abnormal light/dark activity *** for hallmarks related to thalamic input,such as barrels in somatosensory cortex,cortical arealization and laminar maturation proceeded ***,the tangential extension of the mature cortex was diminished,with radial thickness less severely *** layer neurons were reduced in number,and their apical and basal dendritic arbors were blunted,with reduced synapse *** reached the cortex,and their density was comparable with wild *** excitability of mutant pyramidal neurons,measured in vitro in patch-clamp experiments in acute slices,was ***,their firing activity in vivo was quite similar to the wild type,except for the presence of rapid firing exhaustion in some mutant *** field potential and electrocorticogram showed similar range of oscillations,albeit with higher frequency peaks and reduced left-right synchrony in the ***,"protomap" formation,namely cortical lamination and arealization,proceed normally in absence of extrinsic connections,but survival of projection neurons and acquisition of mature morphological and some electrophysiological features depend on the establishment of normal cortical-subcortical relationships.

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Effect of chronic methylphenidate exposure on NMDA receptor phosphorylation and expression in the medial prefrontal cortex of rats

Effect of chronic methylphenidate exposure on NMDA receptor ...

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Shu-Su LIU~1,Xiao-Ting HE~1,Hou-Cheng ZHOU~1,Bao-Ming LI~(1,2,*),Xue-Han ZHANG~(1,*) 1 Institute of Neurobiology & State Key Laboratory of Medical Neurobiology,Institutes of Brain Science,Fudan University,Shanghai 200032,China 2 Center for Neuropsychiatric Diseases,Institute of Life Science,Nanchang University,Nanchang 330031,China

Methylphenidate is widely used for the treatment of Attention-Deficit/Hyperactivity Disorder(ADHD),but the molecular mechanisms of its therapeutic action are not well *** we examined the phosphorylation and expression of NMDA receptor NR2B subunits in order to establish the contribution of these mechanisms in the pharmacotherapy of *** found that chronic exposure to methylphenidate down-regulated synaptic NR2B subunits but were associated with increases in the levels of phosphor-Y-1472 NR2B in the synaptic membrane fractions of rat medial prefrontal cortex(mPFC).However,the total of NR2B subunits in mPFC was *** data suggest that changes of NMDA receptor may be important for cognitive function improvement in response to methylphenidate.

关键词： methylphenidate ADHD NMDA receptor NR2B subunits mPFC

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Generation of temporal codes by elementary thalamocortical circuits in auditory cortex

Generation of temporal codes by elementary thalamocortical c...

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Lukas Mesik Zilkha Neurogenetic Institute Keck School of MedicineUniversity of Southern CaliforniaLos AngelesCA 90089USA

Temporal codes in the form of relative spike latency can efficiently encode sensory *** implementation of such coding strategy requires a broad dynamic range of spike latencies in order to achieve sufficient coding capacity and *** generation of spike latency codes in the sensory pathway remains largely *** report that in individual auditory cortical neurons,spike latency codes for sound frequency cannot be simply attributed to thalamocortical relays,but are primarily generated de novo by local circuits,which fine-tune the integration time for spike generation after the arrival of thalamocortial *** tuned recurrent excitation shortens integration time selectively at the optimal frequency,while broadly tuned inhibition prolongs it preferentially at off-optimal *** push-and-pull antagonistic modulations greatly sharpen spike latency tuning and expand its dynamic ***,thalamocortical circuit-like elementary synaptic circuits can convert information spatially coded in distributions of synaptic strength into temporal codes.

关键词： auditory cortex first spike latency frequency tuning latency coding synaptic circuitry in vivo whole-cell recording

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Role of miRNAs in oligodendrocyte myelination

Role of miRNAs in oligodendrocyte myelination

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Kang ZHENG,Meng-Sheng QIU~* The Institute of Developmental and Regenerative Biology,Hangzhou Normal University,Hangzhou 310036,China

Objective Oligodendrocytes are macroglia which form myelin sheaths in the CNS,and the integrity of myelin is essential to rapid and accurate transmission of action *** pathogenic factors,such as the autoimmunity and mechanical injury,can lead to severe *** oligodendrocyte progenitor cells are present in the adult CNS,they fail to remyelinate *** regeneration to some extent recapitulates development, understanding the molecular mechanism underlying developmental myelination is essential for experimental designs to promote axonal *** this study,we investigated the function role of miRNA in myelination in hope for unraveling molecular cues for enhancing *** Conditional disruption of miRNAs in oligodendrocyte progenitor cells(CNPDicer)and analyzing its effect on ***（1）By blocking the miRNA maturation,there is no obvious effect on proliferation and maintenance of oligodendrocyte progenitor cells;（2）Oligodendrocyte differentiation is slightly delayed in CNPDicermutant mice.（3）Loss of miRNA has little effect on sustaining integrity of myelin *** miRNAs fine-tune oligodendrocyte maturation,but are not essential for axonal myelination.

关键词： oligodendrocyte myelination miRNA Dicer

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Identification of radial glia cells from rat embryonic cerebral cortex

Identification of radial glia cells from rat embryonic cereb...

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Hao-Ming LI,Guo-Hua JIN~*,Jian-Bing QIN,Wei-Wei YANG,Mei-Ling TIAN,Xue-Feng TAN,Xin-Hua ZHANG, Jin-Hong SHI,Lin-Qing ZOU Department of Anatomy and Neurobiology,the Jiangsu Key Laboratory of Neuroregeneration,Nantong University, Nantong 226001,China

Objective Radial glia cells(RGCs) play an essential role during corticogenesis,whereas the precise distribution and contribution to cerebral cortex remains less *** In this research,we used Vimentin as an astroglia marker and Sox2 as a stem/progenitor maker to identify *** The Sox2+ cells in E16.5 cerebral cortex germinal zone co-localized with Vimentin or Nestin positive radial processes which extended to the pial *** vitro,the E16.5 cerebral cortex-derived Sox2+ radial glia(RG)-like cells shared some properties with RGCs in vivo,and these E16.5 RG-like cells displayed multiple differentiation *** Taken together,we identified some characterizations and properties of Sox2+ RGCs derived from rat embryonic cerebral cortex.

关键词： radial glia cells cerebral cortex Sox2 vimentin rat

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Monitoring dynamic protein-protein interactions during GABA,receptor mediated IGF-IR transactivation by an activity based probe

Monitoring dynamic protein-protein interactions during GABA,...

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Fa-Jun NAN~2,Jian-Feng LIU~1 1 Sino-France Laboratory for Drug Screening,Key Laboratory of Molecular Biophysics of Ministry of Education, College of Life Science and Technology,Huazhong University of Science and Technology,Wuhan,China 2 National Center for Drug Screening,State Key Laboratory of Drug Research,Shanghai Institute of MateriaMedica, Shanghai Institutes of Biological Sciences,Chinese Academy of Sciences,Shanghai,China

Many GPCR agonists can activate receptor-tyrosine-kinases（RTKs） through transactivation,the mechanisms of which are poorly ***,we have investigated IGF-1 receptor（IGF-IR） transactivation by GABA receptor using a chemical biology approach in rat primary *** show that GABA receptor activation induces a dynamic assembly and disassembly of a protein complex,including both receptors and their downstream ***,a non-receptor tyrosine kinase,plays a key role in coordinating this dynamic ***,such a dynamic process is critical for transactivation and transactivation-dependent neuronal *** results reveal an important mechanism underlying GPCR transactivation of RTKs.

关键词： GABAB receptor IGF-IR transactivation FAK dynamic protein-protein interactions activity-based probe

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Neural correlates underlying incubation effects in divergent creative thinking:An EEC alpha oscillation study

Neural correlates underlying incubation effects in divergent...

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Mark Bodner MIND Research Institute Santa AnaCAUSA

The phenomenon that new better solutions pop into one's mind after putting an unsolved problem aside for a period of time is called the incubation *** the present study,we tried to investigate neural correlates underlying this effect in divergent creative *** performed Alternative Uses Tasks(AUTs) under the control condition(continuous task performance) and the incubation condition(an incubation period,interpolated task,added) respectively while EEG signals were *** results showed that after an incubation period the subjects provided more creative ideas,and the upper alpha(10-12 Hz) oscillations in the control condition increased at left frontal-central and right parietal-occipital recording sites but such increase was not observed in the incubation *** event related synchronization(ERS) has recently been hypothesized to be a sign of active cognitive processes,reflecting a positive functional correlate of top-down control in relevant neuronal ***,the incubation effect seems to restrain the growth of top-down control and keep the brain sensitive to competing and irrelevant information,which results in more unique ideas in AUT performance.

关键词： Incubation effect divergent creative thinking alternative uses task EEG alpha oscillation

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