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Epileptic electric source localization based on fMRI network information

Epileptic electric source localization based on fMRI network...

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： De-Zhong YAO~*,Xu LEI,Cheng LUO Key Laboratory for Neurolnformation of Ministry of Education,Univeristy of Electronic Science and Technology of China,Chengdu 610054,China

Objective The epileptic source localization is an important issue for diagnostics and control of epileptic *** better method has be the major goal in this field for a long *** this work,based on the simultaneously recorded EEG and fMRI,we are aimed at developing novel source localization method based on EEG-fMRI information *** By utilizing Bayesian model,a new epileptic EEG source localization method,network-based source imaging(NESOI),is desinged which adopts the fMRI network information obtained by independent component analysis as the spatial *** Compared with other typical source localization methods on simulation data,NESOI demnostrates itself with the smallest localization error,then an illustration application to a real epileptic data shows that NESOI may result in a more physiologically reasonable source localization result than the fMRI SPM *** The EEG-fMRI fusion based novel method NESOI is a potentially useful tool for clinic practice in epileptic disorder.

关键词： source localization simultaneously recorded EEG and fMRI brain network bayesian model

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Regional atrophy of the basal ganglia and thalamus in idiopathic generalized epilepsy

Regional atrophy of the basal ganglia and thalamus in idiopa...

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Hanjian DU,Hua FENG Southwest Hospital

Background Earlier studies found that gray matter volume in the frontal,parietal and temporal lobes is significantly altered in idiopathic generalized epilepsy(IGE).Research has indicated that a relationship exists between the brain's subcortical structures and ***,little is known about possible changes in the subcortical structures in *** To determine the changes in the shape of subcortical structures in IGE using vertex *** Fourteen male patients with IGE and 28 age- and sex-matched healthy controls were included in this study,which used high-resolution magnetic resonance *** performed a vertex-based shape analysis, in which we compared patients with IGE with the controls,on the subcortical structures that we had obtained from the MRI *** Statistical analysis showed significant regional atrophy in the left thalamus,left putamen and bilateral globus pallidus in patients with *** These results indicate that regional atrophy of the basal ganglia and the thalamus may be related to seizure *** the future,these findings may prove useful for choosing new therapeutic regimens.

关键词： IGE thalamus globus pallidus atrophy

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miRNAs are important regulators of astrocyte development

miRNAs are important regulators of astrocyte development

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Qing-Ting CHI,Kang ZHENG,Meng-Sheng QIU~* The Institute of Developmental and Regenerative Biology,Hangzhou Normal University,Hangzhou 310036,China

Objective Many CNS pathologies are associated with changes in the structure and function of astrocytes. When the lesioned brain and spinal cord tissues undergo the repairing process,astrocytes reactivate and form glial scars that have a detrimental effect on neural *** the molecular regulation of astrocyte development could help to prevent astrogliosis in CNS ***,at present,little is known about the molecular mechanisms underlying astrocyte *** this study,we attempted to explore the functional role of miRNAs in astrocyte differentiation during *** We generated the GFAP Dicer mice line to block the miRNA function in differentiating astrocytes,and analyze the differential expression of glial specific molecular markers in the spinal cord between mutant and control ***（1） Disruption of miRNAs delays astrocyte maturation;（2） Absence of miRNAs in astrocyte can interfere with oligodendrocyte myelination;（3） miRNAs contribute to the formation and maintenance of astrocyte structure and *** miRNAs are important regulators of astrocyte development.

关键词： astrocyte miRNA spinal cord differentiation

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Enhanced holistic processing in pure alexia

Enhanced holistic processing in pure alexia

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Hanyu SHAO~1,Zhongqin CHEN~2,Xuchu WENG~(1,*) 1 Hangzhou Normal University,Hangzhou 310015,China 2 Zhejiang University,Hangzhou 310008,China

Objectives Damage to the left occipito-temporal cortex often leads to pure alexia,which is characterized by an extreme slowness in *** is well known that the left hemisphere(LH) is specialized for analytic processing and the RH for holistic *** thus hypothesize that the processing of visual words in patients with pure alexia might rely more on holistic process than in normal *** We tested a patient with pure alexia and a group of young controls(n = 32) using a character composite paradigm,which allows the measurement of failures of selective attention to parts of a character that result from a holistic processing *** this paradigm,participants saw pairs of Chinese characters presented briefly and then made a same/different judgment about the attended halves of the two *** Holistic processing was revealed by the interference from the unattended *** patient showed a larger interference effect than the *** Enhanced holistic processing of characters may be a characteristic hallmark of Chinese pure alexia.

关键词： pure alexia holistic processing character composite effect

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Non-apoptotic role of caspase-3 in synapse refinement

Non-apoptotic role of caspase-3 in synapse refinement

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Fei CHEN,Jin-Yuan WANG,Nan-Jie RUAN,Lei QIAN,Bao-Yu DUAN,Zhen-Ge LUO~* Institute of Neuroscience and Key Laboratory of Neurobiology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200031,China

During the development of vertebrate neuromuscular junction,the formation and maintenance of acetylcholine receptor(AChR) clusters are regulated by both positive and negative *** stabilizes,whereas acetylcholine(ACh) destabilizes AChR *** mechanisms underlying this counteractive interaction are not completely *** we show that caspase-3,an effector caspase involved in apoptosis,negatively regulates AChR *** found that caspase-3 was activated by cholinergic stimulation of cultured muscle *** activation did not depend on calpain,a calcium-dependent protease which is involved in the dispersion of AChR *** treatment prevented the activation of ***,inhibition or down-regulation of caspase-3 attenuated ACh agonist-induced dispersion of AChR ***,the loss of AChR clusters in agrin mutant mice was partially rescued by the inhibition of caspase-3 or genetic ablation of *** together, these results suggest a novel role of caspase-3 in the refinement of synapse.

关键词： Non-apoptotic role of caspase-3 in synapse refinement

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Synchrotron radiation based-FTIR microspectroscopy study of the striatum in 6-hydroxydopamine rat model of Parkinson's disease

Synchrotron radiation based-FTIR microspectroscopy study of ...

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Hong-Yan ZHU~*,Xiao PEI,Ling-Yan WU Lab.of Neuropharmacology and Neurotoxicology,School of Life Sciences,Shanghai University,Shanghai 200444, China

In the present study,synchrotron radiation-based Fourier transform infrared(FTIR) was used to analyze the biochemical composition of the striatal neurons in normal and Parkinson's disease(PD) rat brain *** rat model of PD was established by 6-hydroxydopamine destroying the nigrostriatal *** detailed spectral analyses showed significant changes of cellular compositions such as lipids,proteins and nucleic acids in PD neurons with respect to *** a result,the intensities of spectral absorption assigned to lipid in the striatal neurons of PD were higher than that of ***,the unsaturation level of phospholipids decreased for PD neurons with respect to normal,indicating the high level of lipid *** analysis of protein secondary structure showed a significantly higher ratio of P-sheet in PD neurons compared to that of ***,the content of nucleic acids in PD neurons decreased in comparison to *** finding suggest that the biochemical changes in the striatal neurons might be involved in the pathogenesis of PD.

关键词： fourier transform infrared Parkinson's disease

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Update on susceptibility genes in childhood absence epilepsy

Update on susceptibility genes in childhood absence epilepsy

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Yu-Wu JIANG Peking University First Hospital

＜正＞Genetic factor has long been considered to be related to idiopathic epilepsies and the susceptibility gene for idiopathic generalized epilepsy（IGE） has grown to more than 20 genes *** vast majority of them are genes encoding channels or the proteins regulating the action of excitatory or inhibitory neurotransmission in the central nervous system（CNS）.But,these genes mutations can only explain the minor part for idiopathic epilepsy,and the genes responsible for the most common forms of idiopathic epilepsy still remain mostly *** advances recently have resulted in new high-throughput approaches that promise to accelerate the identification of new susceptibility genes and copy number variations（CNVs） for *** this talk,the recent advance of the susceptibility genes study in childhood absence epilepsy（CAE） will be reviewed,especially the two new genes probably associated to Chinese CAE patients we found by combining the whole genome SNP chip association study and direct sequence of the *** studies have revealed recurrent CNVs related to *** study showed the 15q 11.2 might be the most common CNV in Chinese CAE patients,it will be introduced.

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Purine-mediated responses in microglia

Purine-mediated responses in microglia

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Shu-Min DUAN Zhejiang University School of Medicine,Hangzhou 310058,China

Microglia are resident immune cells in the central nervous system(CNS).In response to injury or inflammatory stimuli,the resting microglia can be rapidly activated to participate in pathological responses,including migration to the affected site,release of various inflammatory molecules,and clearing of cellular *** released from damaged neural cells has been considered to be a major chemokine for inducing rapid process extension and migration of microglial ***,ATP leaking from the local injured cells may not be able to diffuse extensively to effectively induce migration of remote microglia,because extracellular nucleotides are rapidly degraded by ecto-ATPases in the *** found that ATP-indueed ATP release from microglial lysosomes contributed to the directional migration of remote *** addition,we found that ATP could function as a "drink" signal in microglia by triggering ***,we found that this purine-induced macropinocytosis is very efficient in clearing up extracellular soluble amyloid beta(Aβ),an early and key intermediate in the pathogenesis Alzheimer's disease.

关键词： ATP Purine-mediated responses in microglia

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Using Tol2-Gal4 mediated enhancer trap to realize targeted gene expression in zebrafish

Using Tol2-Gal4 mediated enhancer trap to realize targeted g...

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： He-Fei ZHANG,Nan XU,Si-Jie CHEN,Gang PENG~* Institutes of Brain Science and State Key Laboratory of Medical Neurobiology,Fudan University,Shanghai 200032, China

Targeted gene expression is a powerful approach in studies of the nervous *** have used Tol2 mediated enhancer trap to find tissue specific enhancers in zebrafish genome and to produce transgenic driver lines expressing Gal4 protein in specific parts of ***4 protein can recognize upstream activator sequence(UAS)and activate the expression of UAS-driven reporter *** 35 driver lines that express Gal4 in specific parts of CNS have been screened *** have also mapped the loci of Tol2-Gal4 insertions in some of our driver *** of the insertion loci are located near developmentally important genes such as *** driver lines will be powerful tools for neuronal development research and behavior studies.

关键词： enhancer trap targeted gene expression Tol2-Gal4 UAS

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新生大鼠脑内DNA甲基化结合蛋白表达及神经发育的研究

新生大鼠脑内DNA甲基化结合蛋白表达及神经发育的研究

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者：刘芳倪晶晶孙凤艳复旦大学医学神经生物学国家重点实验室复旦大学生物医学研究院干细胞研究所

MeCP2作为一种在Rett综合症中起到关键作用的转录抑制因子,已经受到研究者们的广泛关注,其磷酸化蛋白pMeCP2(phosphorylated MeCP2)在脑内的功能也已被初步揭示。然而,MeCP2及pMeCP2在新生大鼠脑发育过程中的表达情况及其在细胞内的定... 详细信息

MeCP2作为一种在Rett综合症中起到关键作用的转录抑制因子,已经受到研究者们的广泛关注,其磷酸化蛋白pMeCP2(phosphorylated MeCP2)在脑内的功能也已被初步揭示。然而,MeCP2及pMeCP2在新生大鼠脑发育过程中的表达情况及其在细胞内的定位情况仍不清楚。为了解决这一问题,我们采用胚胎期19天(E19)及出生后1、7、14、30天(P1,P7,P14,P30)的大鼠皮层进行研究,Western blot结果显示,随发育进行,pMeCP2及npMeCP2在皮层中的表达均发生明显变化。其中,pMeCP2在E19的含量最高,并在P1显著下降,随后在P7,P14逐渐增多,最后在P30显著减少至最低值。与此同时,在E19、P1、P7和P14天,npMeCP2的变化趋势与pMeCP2类似;然而在P30天,npMeCP2表达量持续升高至最高值。此外,免疫组织化学结果显示,在P7、P14、P30大鼠的皮层,海马和小脑组织中,pMeCP2及npMeCP2的表达也具有随发育进行逐渐变化的特点。值得注意的是,在大鼠皮层发育过程中,神经元和星形胶质细胞标记蛋白以及其他相关的蛋白因子也具有各自的发育变化特点。以上结果提示我们,pMeCP2及npMeCP2可能参与了大鼠的脑发育过程。

关键词： MeCP2 磷酸化脑发育

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