Optokinetic response (OKR) is a reflexive response which is elicited by a slowly moving image across the *** stimulation is generated by a drum with black-white vertical stripes on its internal wall horizontally rotat...
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Optokinetic response (OKR) is a reflexive response which is elicited by a slowly moving image across the *** stimulation is generated by a drum with black-white vertical stripes on its internal wall horizontally rotating around the *** treat 8-9 dpf larvae by either egg water or drugs for an hour before the *** larvae are then transferred to a 3.5 cm-diameter petri dish containing 6% methylcellulose at the center of the *** of the optokinetic responses are captured by CCD camera and processed by Image-J to analyze the effects of anxiolytic drugs on visual system.
Microglia,the primary immune effector cells in the brain,are critically involved in many pathological conditions including injury,stroke,and Alzheimer's and Parkinson's *** physiological condition, microglia s...
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Microglia,the primary immune effector cells in the brain,are critically involved in many pathological conditions including injury,stroke,and Alzheimer's and Parkinson's *** physiological condition, microglia spend most of time on a resting state with highly branched processes surveying surround brain area. However,little is known about what are sensed by resting microglia and what are their physiological *** in vivo two-photon morphology and calcium imaging in the optic tectum of zebrafish larvae,we found here that the bulbous contact between neuron and microglial process was positively correlated with spontaneous neuronal activity. Local increase of neuronal activity by two-photon based focal glutamate uncaging effectively induced orientedmotility of resting microglial processes and its bulbous contact with neurons in uncaged ***,microglial contact decreased spontaneous neuronal ***,resting microglia dynamically surveying high neuronal activity are important for neuronal activity regulation and homeostasis in vivo.
Neurons are the most vulnerable cells upon ischemic *** of neuronal survival is thus important for prevention of ischemic brain *** report that the transient receptor potential canonical(TRPC) 6 was neuroprotective in...
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Neurons are the most vulnerable cells upon ischemic *** of neuronal survival is thus important for prevention of ischemic brain *** report that the transient receptor potential canonical(TRPC) 6 was neuroprotective in focal cerebral ***6 protein level in the neurons was greatly reduced in *** downregulation was specific for TRPC6 in the members of TRPC subfamily and mediated by calpain proteolysis.A peptide that contains a sequence spanning calpain cleavage site on TRPC6 protein specifically blocked TRPC6 ***,this peptide was neuroprotective against ischemic insults in both cultured neurons and *** level of phosphorylated cAMP-response element binding protein(pCREB) was maintained by the peptide,suggesting that the CREB signaling pathway was involved in the neuroprotective *** results indicated that suppression of calpain-mediated TRPC6 degradation preserved neuronal survival and prevented ischemic brain damage.
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