Aim: Voltage-gated calcium (Ca)channels play a key role in the control of heart function andare also essential for normal heart development. Five different calcium channel α1 subunitsconferring L-type （Ca1.1, Ca1.2,...
详细信息
Aim: Voltage-gated calcium (Ca)channels play a key role in the control of heart function andare also essential for normal heart development. Five different calcium channel α1 subunitsconferring L-type （Ca1.1, Ca1.2, Ca1.3） and T-type currents （Ca2.1 and Ca2.2） have beendemonstrated to be expressed in the adult heart. We have shown previously that the intact Ca1.2gene is required for normal embryonic development after day 13 p.c., and that murine embryoshomozygous for the deletion of Ca1.2 die before day 14.5 post coitum （p.c.）. In this study weinvestigated the changes in the mRNA levels of the principal Cachannel α subunits duringmouse embryogenesis between embryonic days 9.5 and 15.5. Finally, in an attempt to study the
Pyrrolidine dithiocarbamate (PDTC) is a potent inhibitor of nuclear factor kappa B (NF-□B). Wehave previously reported that the effects of pyrrolidine dithiocarbamate(PDTC) on cell death andNF-□B inhibition are zinc...
详细信息
Pyrrolidine dithiocarbamate (PDTC) is a potent inhibitor of nuclear factor kappa B (NF-□B). Wehave previously reported that the effects of pyrrolidine dithiocarbamate(PDTC) on cell death andNF-□B inhibition are zinc dependent(Kim et al, FEBS Lett. 449, 28-32, 1999, Kim, et al, ***. 72, 1586-1592, 1999), not through its antioxidant effect. In the present study, wesought to characterize the molecular mechanism of PDTC inhibition of NF-□B activity. PDTC didnot alter NF-□B binding to DNA, bat prevented the translocation of the p65 subunit of NF-□Binto the nucleus. As an inhibitor of NF-□B, PDTC paradoxically activated I□B kinase (IKK).But, degradation of phosphorylated I□B□was blocked by PDTC. PDTC also stabilized andincreased p53 and p21 proteins that are degraded by proteasome. A highly specific proteasome
暂无评论