提取高质量条斑紫菜基因组DNA(天根新型植物基因组DNA提取试剂盒,DP320-02,北京),Covaris S2(Covaris Inc.)超声仪进行片段化。采用(NEB Next DNA Sample Prep ReagentSetl,New England biolabs Inc.)进行末端补平,3’末端单碱基"A...
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提取高质量条斑紫菜基因组DNA(天根新型植物基因组DNA提取试剂盒,DP320-02,北京),Covaris S2(Covaris Inc.)超声仪进行片段化。采用(NEB Next DNA Sample Prep ReagentSetl,New England biolabs Inc.)进行末端补平,3’末端单碱基"A"加尾,adapter连接和PCR富集,QIAgen MinElute PCR Purification Kit纯化。纯化产物NanoDropND-1000(ThermoScientific Inc.)定量后按照Solexa测序仪标准流程进行2×81双向测序。根据Solexa序列质量值,去除两个或两个以上连续低质量碱基(Q值小于20),只保留长度大于35 bp的序列。SOAPdenovo(v1.04,BGI)对高质量Solexa序列进行拼接,参数设定为:K-mer长度29bp,文库平均插入长度300bp,scaffolding联配长度35bp,其余参数默认。拼接完成后使用gapcloser程序进行scaffold内部空洞填补。质量过滤后共得55,749,075条总计4,171,821,694 bp高质量序列。SOAPdenovo程序拼接并根据序列正反向和文库大小信息构建scaffold和填补序列空洞,结果得到:长度587,042个大于100 bp的contig,总长170,619,909 bp,N50值401 bp,平均GC含量为53.08%,最大contig 44,754 bp;370,119个scaffold总长220,854,049 bp,N501,317 bp,最大scaffold 236,183 bp。
Colony formation is highly import ant for the competitive advantage of the cyanobacterium Microcystis overother phytoplankton *** laboratory-grown colonial Microcystis strains isolated from Lake Taihu（China） maintai...
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Colony formation is highly import ant for the competitive advantage of the cyanobacterium Microcystis overother phytoplankton *** laboratory-grown colonial Microcystis strains isolated from Lake Taihu（China） maintained colonial forms under the low light condition(10μE m s).The cell surface hydrophobicities of the Microcystis colonies were measured by cyanobacterial adherence to xylene in comparison
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