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检索条件"任意字段=中国藻类学会第八次会员大会暨第十六次学术讨论会"

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汕头南澳白沙湾浮游植物群落结构及水体营养盐分布特征

汕头南澳白沙湾浮游植物群落结构及水体营养盐分布特征

引用

中国藻类学会第八次会员大会暨第十六次学术讨论会

作者：张俊梅杨宇峰暨南大学水生生物研究所水体富营养化与赤潮防治广东省教育厅重点实验室

2010年春季和秋季分别对汕头深澳湾4个不同功能区水体的浮游植物及营养盐进行了调查,共发现浮游植物5门71属152种,其中硅藻门46属97种,甲藻门13属34种,绿藻门7属9种,蓝藻门2属2种,硅藻是该海域浮游植物群落优势类群。优势种、属为:角毛... 详细信息

2010年春季和秋季分别对汕头深澳湾4个不同功能区水体的浮游植物及营养盐进行了调查,共发现浮游植物5门71属152种,其中硅藻门46属97种,甲藻门13属34种,绿藻门7属9种,蓝藻门2属2种,硅藻是该海域浮游植物群落优势类群。优势种、属为:角毛藻属(Chaetoceros)、中肋骨条藻(Skeletonema costarum)、拟菱形藻属(Pseudonitzschia)和海链藻属(Thalassiosira)等。调查期间,浮游植物数量最高值均出现在对照区表层,最低值出现在贝类养殖区的微表层。其中,春季浮游植物细胞密度最高达6.44×10 cells/L,最低为2.32×10 cells/L;秋季浮游植物细胞密度最高达600.91×10c ells/L,最低为7.26×10cells/L。调查结果显示,浮游植物密度的空间分布趋势为非养殖区大于养殖区(P<0.05),呈现湾外向湾内递减的趋势;而时间分布趋势为秋季浮游植物细胞密度高于春季(P<0.05)。各养殖区多样性指数和均匀度指数介于1～3和0.3～0.5之间。按照Shannon-Wiener指数和均匀度指数评价,养殖区属于中营养型水体,对照区则介于贫营养型和中营养型之间。调查显示,春季溶解性无机氮(DIN)和总氮(TN)含量高于秋季,而活性磷酸盐和总磷(TP)含量则低于秋季。DIN组成以硝态氮(NO-N)为主,其次为氨氮(NH-N)。夏季各功能区DIN含量平均为17.74±5.78μmol/L,秋季平均为11.96±2.41μmol/L,根据国家《海水水质标准》(GB3097-1997),按照DIN含量划分,夏季各功能区水质符合Ⅱ类水标准,秋季则达Ⅰ类水标准。

关键词：白沙湾浮游植物营养盐

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Composition of photosynthetic organisms and diurnal changes of photosynthetic efficiency in algae and moss crusts

Composition of photosynthetic organisms and diurnal changes ...

引用

中国藻类学会第八次会员大会暨第十六次学术讨论会

作者： Shubin Lan,Li Wu,Chunxiang Hu~* Institute of Hydrobiology,Chinese Academy of Sciences,430072,Wuhan

＜正＞Aims In Dalateqi region of Inner Mongolia（at eastern edge of Qubqi Desert）, man-made algae crusts were constructed with two filamentous *** to three years later,the algae crusts began to succeed to moss crusts under some microterrain *** order to characterize the community structure and compare the photosynthetic characteristics in different successional stages of biological soil crusts（BSCs）.Methods This paper studied composition of photo-synthetic organisms and diurnal changes of photosynthetic efficiency,with microscopic observation and chlorophyll fluorescence monitoring *** Compared with the early successional algae crusts,later moss crusts had a higher photosynthetic efficiency, which was closely related with the different community structure of photosynthetic ***,moss crusts had a higher total photosynthetic biomass,although the algal biomass decreased along the successional *** to higher vascular plants,a midday depression of photosynthetic efficiency was detected in BSCs,although the depression extents were different in different successional *** demonstrated the depression was mainly caused by non-stomata limitations,and photosynthetically active radiation（PAR） was a significant influencing factor in the down-regulation of photo-synthetic *** We conclude, cyanobacterial inoculation effectively promotes the formation,development and seccession of ***,the later successional BSCs have a better photosynthetic performance and thus are expected to play a more effective role in desertification control.

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低温诱导缺刻缘绿藻ω3FAD基因在酿酒酵母中的表达

低温诱导缺刻缘绿藻ω3FAD基因在酿酒酵母中的表达

引用

中国藻类学会第八次会员大会暨第十六次学术讨论会

作者：李慧周志刚上海海洋大学水产与生命学院上海高校水产养殖E-研究院

ω3脂肪酸去饱和酶(ω3 fatty acid desaturase,ω3 FAD)可以使藻类细胞产生一系列ω3脂肪酸。在已克隆到缺刻缘绿藻(Myrmecia incisa)的ω3 FAD基因(GenBank登录号:EU658930)基础,为进一步了解其功能,设计含有酶切位点的引物,利用PCR...

ω3脂肪酸去饱和酶(ω3 fatty acid desaturase,ω3 FAD)可以使藻类细胞产生一系列ω3脂肪酸。在已克隆到缺刻缘绿藻(Myrmecia incisa)的ω3 FAD基因(GenBank登录号:EU658930)基础,为进一步了解其功能,设计含有酶切位点的引物,利用PCR技术扩增得到开放式阅读框(ORF)片段,基于pYES2质粒构建了ω3FAD基因的表达载体pY-ω3FAD,电穿孔法将pY-ω3FAD质粒转入酿酒酵母(Saccharomyces cerevisiae)INVSc1菌株,应用尿嘧啶缺陷的合成培养基(SC-U)筛选并经PCR扩增目的基因、序列验证后获得含有pY-ω3FAD重组载体的酵母转化株。将在YPD培养基中培养至对数生长期的转基因酵母,添加外源脂肪酸底物,并透过半乳糖诱导,在5℃、10℃、15℃、20℃、25℃及30℃等不同温度下培养。脂肪酸成分的气象色谱(GC)分析结果表明,与野生型酵母相比,在30℃培养的转基因酵母中,没有检测到新的产物;但转基因酵母在低温条件下培养时,经与标准脂肪酸对照及气质联用(GC-MS)证明,能将外源添加的亚油酸(linoleic acid,C18:2,LA)去饱和为α-亚麻酸(α-linolenic acid,C18:3,ALA);且随着温度降低,去饱和能力增强,在实验设计的温度梯度实验中,5℃时的转化效率达到29.73%。在5℃条件下,添加外源脂肪酸后于1d、2d、3d及4d不同时间诱导培养,发现随着诱导培养时间增加,转化效率也提高;培养4 d的转基因酵母,能将38.86%亚油酸去饱和为α-亚麻酸。本研究为缺刻缘绿藻ω3 FAD基因的功能鉴定及脂肪酸合成代谢途径的深入探讨奠定了基础。

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太湖蓝藻水华早期暴发特性及监测指标

太湖蓝藻水华早期暴发特性及监测指标

引用

中国藻类学会第八次会员大会暨第十六次学术讨论会

作者：周全陈伟万能宋立荣中国科学院水生生物研究所常熟理工学院生物与食品工程系

太湖位于江苏与浙江两省交界处,长江三角洲南部,是中国第三大浅水湖泊。近年来,由于区域经济的快速发展以及人为活动的频繁干扰,每年都会出现严重的蓝藻水华。太湖水华蓝藻以微囊藻为主要优势属,水华暴发期通常从5月持续到11月,每年的3... 详细信息

太湖位于江苏与浙江两省交界处,长江三角洲南部,是中国第三大浅水湖泊。近年来,由于区域经济的快速发展以及人为活动的频繁干扰,每年都会出现严重的蓝藻水华。太湖水华蓝藻以微囊藻为主要优势属,水华暴发期通常从5月持续到11月,每年的3月至6月是太湖微囊藻水华复苏和早期暴发的关键时期。通过连续三年在该时期对太湖北部梅梁湾和竺山湾水域进行高频次采样监测,本论文围绕太湖微囊藻水华复苏和早期暴发阶段的藻类光合系统Ⅱ活性、藻类色素浓度、溶解性胞外多糖浓度以及水温、水体电导率、氮、磷等多项理化因子进行了研究,归纳并初步遴选了用于太湖蓝藻水华形成和早期暴发预警系统的监测指标。研究结果表明,采样监测期间太湖微囊藻复苏的平均温度为15.6±1.4℃,初次水华暴发的平均温度为24.1±2.1℃,水华微囊藻（Microcystis flos-aquae）是太湖蓝藻水华早期发生阶段的主要种类;在微囊藻复苏阶段,底泥中藻类光合系统II活性首先上升,随后底层水中藻蓝蛋白浓度增加,水体中出现直径以100μm至200μm为主的微囊藻群体;在初次水华的形成阶段,水体中藻类光合系统Ⅱ活性（△F/Fm’值）在0.151至0.674范围内变动,并逐渐出现更大直径的群体,△F/Fm’的最高值通常出现在春季微囊藻水华初次爆发前数日,表明复苏的微囊藻已经在水体中快速增殖,已初具发生水华的潜力。分析发现,水温、溶解性胞外多糖浓度、水体电导率、碱性磷酸酶活性、藻类光合系统Ⅱ活性等指标的变化与太湖微囊藻水华的发生具有较为密切的关系。根据水华早期形成与暴发过程的不同阶段,可将这些关键指标按照其敏感指示期划分为不同层次的指标体系,从而使之具有一定的可操作性和推广应用前景。本研究对于湖泊蓝藻水华预警系统的建立和完善具有重要意义,有利于地方政府或相关部门对蓝藻水华的暴发进行预告或采取相应防护措施,其中部分监测指标仍有待进一步论证,同时验证其适用范围与可行性。

关键词：太湖微囊藻水华监测指标水华预警

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我国东海赤潮高发区甲藻孢囊种类多样性和生态地理分布

我国东海赤潮高发区甲藻孢囊种类多样性和生态地理分布

引用

中国藻类学会第八次会员大会暨第十六次学术讨论会

作者：黄琳吕颂辉王艳暨南大学生命科学与技术学院赤潮与水环境研究中心

2010春季（4、5月份）在东海赤潮高发区14个站点采集表层沉积物样品,进行甲藻孢囊的种类多样性和生态地理分布研究。研究共采集鉴定出45种孢囊,各站位孢囊种类数为2～34种,平均22种;孢囊密度为1.65～395.73 cysts/g D Wt,平均密度为139.... 详细信息

2010春季（4、5月份）在东海赤潮高发区14个站点采集表层沉积物样品,进行甲藻孢囊的种类多样性和生态地理分布研究。研究共采集鉴定出45种孢囊,各站位孢囊种类数为2～34种,平均22种;孢囊密度为1.65～395.73 cysts/g D Wt,平均密度为139.69 cysts/g D Wt。其中za6a,zb11,zc17三个站位孢囊种类较为丰富,均达到30种/站位以上,且孢囊密度较高,分别达到244.98、358.79、395.73 cysts/g D Wt。共分析出六大类孢囊,其中包括膝沟藻类孢囊（Gonyaulacoid group）13种,占总孢囊密度为23.67%;Tubercudinioid类孢囊（Tubercudinioid group）1种,占总孢囊密度为1.50%;钙质类孢囊（Calcoidinellid group）4种,占总孢囊密度为22.77%,其中锥状斯氏藻为优势种,其孢囊密度在0.83～134.17 cysts/gD Wt,平均为37.75 cysts/g D Wt,且在zb11站位锥状斯氏藻孢囊密度达到最大值,所占总孢囊密度比例也相对较高;裸甲藻类孢囊（Gymnodinoid group）6种,占总孢囊密度为29.45%;原多甲藻类孢囊（Protoperidinioid group）18种,占总孢囊密度为12.36%;翼甲藻类孢囊（Diplopsalid group）3种,占总孢囊密度为10.24%。而有毒甲藻孢囊（亚历山大藻）密度在0.00～51.26 cysts/g D Wt,平均为18.23cysts/g D Wt。在检测的14个站位中有13个站位发现有亚历山大藻孢囊分布,并且zb11站位亚历山大藻孢囊密度达到最大值,所占总孢囊密度比例也相对较高;gb10站位没有发现亚历山大藻孢囊。rb8,rb12,za3,za6a,zb11,zc17这六个站位的总孢囊密度,孢囊种类数,锥状斯氏藻孢囊密度,亚历山大藻孢囊密度均相对较高,这儿个站点位处于长江口赤潮高发区,富营养化较为严重,适宜的环境条件有利于甲藻形成孢囊并沉积,应该给予特别关注。

关键词：甲藻孢囊多样性生态分布东海有害藻华

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Morphological and molecular characterization of a new species of Thalassiosira (Bacillariophyta) in China

Morphological and molecular characterization of a new specie...

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中国藻类学会第八次会员大会暨第十六次学术讨论会

作者： ZHOU Wei ZHANG Dong SUI ZhengHong YANG Shimin KYOUNG HoKang SANG MinLim Key Laboratory of Marine Genetics and Breeding Ministry of Education Ocean University of China Qin Putian Marine and Fishery Environmental Monitoring Station Putian Marine and Fishery Bureau Putian Department of Aquaculture Chonnam National University Yeosu 323000 Korea

A nanoplanktonic strain was isolated from Taipingjiao of Qingdao, China. The basic morphological character under light microscope observation and the valve structure under electronic microscope observation of the new isolate were similar with those of Thalassiosira allenii Takano, T. partheneia Schrader and T. hispida Syvertsen. However, the new isolate is distinguished from T. allenii in girdle view, density of areola and the number of strutted processes in a circle, from T. partheneia in diameter of the valve, valve view, density of areola, number of supported strutted processes and the place of labiate process, and from T. hispida in density of areola, the number of strutted processes and the absent of a capillary protuberance. Internal view of valve disclosed that the new isolate possessed loculate areolae with individual cribra, semi-continuous cribra on valve face and continuous cribra on valve mantle from internal view of valve surface. Valve face fultoportulae had satellite pore cover and cowlings.

关键词： ITS -rDNA morphology nanoplankton taxonomy Thalassiosira China

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Small-Scale Vertical Distribution of Algae and Structure of Lichen Soil Crusts

Small-Scale Vertical Distribution of Algae and Structure of ...

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中国藻类学会第八次会员大会暨第十六次学术讨论会

作者： Li Wu,Shubin Lan,Chunxiang Hu~* Institute of Hydrobiology,Chinese Academy of Sciences,430072,Wuhan

＜正＞Although many studies have been conducted on the ecological functions and ecophysiological characteristics of lichen soil crusts（LSCs）,no explanation of these results has been provided based on crust *** algae soil crusts（ASCs） as comparison,this work studied the small-scale vertical distribution of algal biomass and stratification in two types of LSCs,by combining the binocular stereomicroscope observations,microscope observations,plate cultures,chlorophyll analysis,and polysaccharides *** results showed an obvious difference in the proportion but not the composition of the algae species between the ASCs and the two LSCs. Approximately 60%and 80%of the total algal biomass were concentrated in the top 1 mm of the soil profile and thalli in the ASCs and LSCs,*** implies that symbiotic algae are the dominant species and primary organic carbon producers in LSCs, and the algal biomass decreased with the depth in both the ASCs and *** small-scale vertical distributions of the crustal algal biomass and polysaccharides were characterized by obvious successional stage,whereas these were unrelated to the crust type within the successional ***,a large amount of fungi,which were always piercing the entire crusts,were observed in the LSCs,but only occasionally in the *** special structures are purported to cause the LSCs to achieve specific ecological functions,such as higher carbon fixation and greater compressive strength. High biomass,large living space,and advantageous resource utilization privilege suggest that the lichen association is mutualistic and the direction from ASCs to LSCs is developmental.

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Characterization and comparative profiling of the microRNAs in two generations of Porphyra haitanensis Chang et Zheng(Bangiales,Rhodophyta)

Characterization and comparative profiling of the microRNAs ...

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中国藻类学会第八次会员大会暨第十六次学术讨论会

作者： Xie Chaotian,Mei Gaoshang,Chen Changsheng,Xu Yan,Ji Dehua (Fisheries College,Jimei University,Xiamen,361021)

Porphyra,one of the most important marine crops in Asia,possesses two heteromorphic life *** studies have found that the expressions of the majority of genes of Porphyra are ***,there is limited knowledge of the regulation expression of these phase-related ***(miRNAs) are a class of endogenous small RNAs that play essential regulatory roles in gene *** gain an insight into the characteristics and expression patterns of miRNAs and to investigate their roles phase-specific expression phases,Small RNAs were identified and quantified from the thallus and conchocelis phases of *** by next generation high-throughput sequencing technology.A total of 2328 known miRNAs were obtained. Comparative profiling revealed that 1191 of the known miRNAs showed significant expression differences between the two *** differentially expressed miRNAs could be divided into three groups,based on their different expressions in the two *** also identified eleven novel miRNAs by Blastn searching against Porphyra *** prediction of all the known miRNAs resulted in a total of 4916 miRNA-targets *** and KEEG annotation revealed that highly ranked miRNA-target genes were those implicated in "translation,ribosomal structure and biogenesis" and "posttranslational modification,protein turnover,chaperones",but not in *** study characterized the miRNA transcriptomes between the thallus and conchicelis phases of *** results provide a broad overview of miRNA expression in the two phases of ***,and lay a foundation for unraveling the miRNA-mediated regulatory networks controlling the development of Porphyra.

关键词： miRNAs Bangiales,Rhodophyta Characterization and comparative profiling of the microRNAs in two generations of Porphyra haitanensis Chang et Zheng microRNAs

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Photosynthetic response of macroalga Gracilaria lemaneiformis(Rhodophyta) to N and P fluctuations as a function of different temperatures

Photosynthetic response of macroalga Gracilaria lemaneiformi...

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中国藻类学会第八次会员大会暨第十六次学术讨论会

作者： Changpeng Ye Yufeng Yang and Ya Zuo Institute of Hydrobiology of Jinan University,Key Laboratory of Eutrophication and Red Tide Control,Education Department of Guangdong Province,Guangzhou 510632,Guangdong Province,China

Gracilaria lemaneiformis,a commercial red macroalga with multiple uses,was cultivated under different combinations of temperatures（10,20 and 30℃） and nutrients （seawater and its different enriched status） to determine its photo-physiological response pattern to the above two combined stress.O evolution-based maximum photosynthetic rate(P) was strongly stimulated by temperature,increased with increasing temperature up to 20-30℃,while showing no significant difference between different N and P treatments under medium nutrient *** N and P treatments inhibited the photosynthetic rate,while showing no significant difference between seawater（CK） and highly enriched seawater（H-NP） *** same trend was followed by the dark respiration rate（R）,light saturation point（I）,and light compensation point(I).The light utilization coefficient（a） was insensitive or decreased slightly with increasing *** chlorophyll fluorescence transients（OJIP） curves of *** and most corresponding specific parameters seemed to be nutrition *** temperature effect on Chi a seemed to be *** every nutrient regime,Phycoerythrin（PE） contents were significantly higher at 10℃relative to that at 20 and 30℃,while showing no significantly difference between different nutrient ***,due to the general high photosynthetic tolerance of *** to the ambient and/or above nutrient-loaded stress at a wide range of temperature,the organism is a more temperatue-releated than nutrient-releated species,and then a potent candidate for bioremediateing in eutrophicated mariculture zones.

关键词： eutrophication Gracilaria lemaneiformis photosynthetic parameters temperatures

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Revisiting the difference of temperature-rate relationships of autotrophic and heterotrophic planktona meta-analysis on intraspecific and interspecific relationships

Revisiting the difference of temperature-rate relationships ...

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中国藻类学会第八次会员大会暨第十六次学术讨论会

作者： Binzhang Chen 1,State Key Laboratory of Marine Environmental Science,Environmental Science Research Center,Xiamen University,361005 Xiamen,Fujian,China

I revisit the possible difference of temperature-growth rate relationships between autotrophic and heterotrophic *** a meta-analysis on published studies on temperature and growth rates of autotrophic and heterotrophic plankton under resource-replete conditions,I find that,while significant heterogeneity exists among phylogenetic groups,the mean temperature coefficient of intraspecific growth rates of phytoplankton defined as the activation energy in the Arrhenius equation is not significantly different from the 'canonic' 0.65 eV predicted by the Metabolic Theory of Ecology,but the mean activation energy of interspecific growth rates of phytoplankton is close to 0.32 eV predicted *** difference of the mean activation energy of intraspecific phytoplankton growth rates from theoretical derivation might result from the widespread C02 concentrating mechanisms in phytoplankton,which minimizes the problem of *** difference of the activation energy between intraspecific versus interspecific phytoplankton growth rates might reflect the strategy of cold-adaptation in phytoplankton,but not in heterotrophic plankton,for which the mean activation energies of both intraspecfic and interspecific growth rates are close to 0.65 *** mean activation energies of intraspecific ingestion and clearance rates of heterotrophic plankton are also insignificantly different from 0.65 *** results suggest that when investigating the response of plankton systems to increasing temperature,the knowledge of the changes in community structure is important.

关键词： Revisiting the difference of temperature-rate relationships of autotrophic and heterotrophic planktona meta-analysis on intraspecific and interspecific relationships

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