纳米量子点(quantum dots,QDs)在生物成像应用和靶向给药等方面呈现出巨大的发展潜力,但组织靶向效率有待提高。本文中,我们根据丝腺组织的氨基酸使用偏好性,将丝蛋白质中含量最丰富的甘氨酸或丙氨酸连接CdTe QDs,显著提高了QDs在无脊椎模型动物家蚕体内的组织靶向转移效率和生物安全性。经口暴露QDs530-Ala or QDs530-Gly后24h,进入循环系统的效率分别比QDs530提高了2.6±0.31倍和1.5±0.29倍。进一步进入靶标组织丝腺,一种蛋白质快速合成和外分泌组织的量,分别提高了7.8±0.89倍和2.88±0.22倍;而进入非靶标组织脂肪体,一种功能类似人类肝脏的量,分别降低了(68.4±4.35)%和(46.7±9.05)%。在组织中QDs530-Ala比QDs530-Gly的纳米颗粒结构完整性更好。背脉管暴露QDs530-Ala,其在循环系统存留时间比QDs530延长,而进入非靶标循环血细胞的速度显著降低。同时,QDs530-Ala有效降低了组织中QDs诱发的ROS量,显著降低了Cd Te QDs对血细胞和生物体的毒性。
表皮作为家蚕个体防御的第一道物理屏障,在高温耐受中发挥重要的作用。化学感应蛋白CSP(Chemosensory Protein,CSP)作为一类重要的化学信号感应蛋白,在免疫反应、生长发育及信号传递中有着重要的作用。高温饲养环境引起家蚕体内的一系列包括激素水平、离子浓度及分子应激等在内的生理生化反应,也可能引起BmCSPs在不同家蚕组织中的表达的变化。本文通过RT-PCR检测了15个BmCSPs在家蚕表皮中的组织特异性表达,并利用半定量RT-PCR和Real time PCR技术分析了高温处理不同时间的表皮中在表皮特异表达的BmCSPs的mRNA表达水平的变化。试验结果显示:在15个BmCSPs中有8个在表皮中特异性表达,包括BmCSP1、BmCSP4、BmCSP6、BmCSP7、BmCSP8、BmCSP9、BmCSP11和BmCSP15;但是高温处理后,表皮中的BmCSP8和BmCSP15的mRNA表达水平显著上调,其它6个BmCSPs的mRNA水平不受高温处理的影响。我们推测BmCSP8和BmCSP15可能与家蚕个体的高温应激反应相关。这些数据为进一步为分析现有的BmCSPs基因在不同家蚕品种高温应激反应中的作用奠定了基础。
In silkworm, Bombyx mori Linnaeus（Lepidoptera: Bombycidae）, blastokinesis results in embryo reversal from ventrally to dorsally convex flexion. In this study, we showed that the extramacrochaetae（emc） gene is requ...
In silkworm, Bombyx mori Linnaeus（Lepidoptera: Bombycidae）, blastokinesis results in embryo reversal from ventrally to dorsally convex flexion. In this study, we showed that the extramacrochaetae（emc） gene is required for blastokinesis in silkworm. Depletion of Bmemc expression via RNA interference led to severe phenotypic defects in blastokinesis. The defective embryos failed to invert their body sides during blastokinesis. This caused the posterior half of the abdomen to abnormally fold back toward the dorsal side, forming a U-shaped morphology. Dorsal closure was also disrupted. Our results suggest that Bmemc is involved in blastokinesis of silkworm embryos.
BmECM25(previously reported as BmVMP25) was previously predicted as a gene encoding the vitelline membrane protein in silkworm, Bombyx mori. In this study, we investigated the detail temporal and spatial patterns of B...
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BmECM25(previously reported as BmVMP25) was previously predicted as a gene encoding the vitelline membrane protein in silkworm, Bombyx mori. In this study, we investigated the detail temporal and spatial patterns of BmECM25 protein. Western blot results showed that BmECM25 was expressed in the follicular epithelium cells from stages-6 to +1, and was then secreted into the oocytes. However, the abundance of BmECM25 decreased during the subsequent oogenesis and finally disappeared in the mature follicles. Immunofluorescence detection showed that BmECM25 locates inside the VM layer and forms a discontinuous layer. These features of BmECM25 suggest that it is an oocyte membrane matrix protein, not a vitelline membrane protein.
In order to improve the utilization ratio of mulberry tea 1-deoxynojirimycin（DNJ）, mulberry tea was prepared based on the common production process of green tea, and corresponding experimental design and statistical...
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In order to improve the utilization ratio of mulberry tea 1-deoxynojirimycin（DNJ）, mulberry tea was prepared based on the common production process of green tea, and corresponding experimental design and statistical analysis were set using Design-Expert software. The results suggested microwave power 602.28 W and processing time 11.41 min were found to be optimal for microwave-assisted extraction DNJ. The confirmatory experiment showed the coincidence rate of detection was 99.58%. It provided a reference for efficient use of mulberry tea DNJ for people who would drink mulberry tea to control blood glucose levels or to prevent diet-induced obesity.
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