Background In this study,we apply a novel echocardiography method,vector flow mapping(VFM),to assess the flow field changes of the right ventricle in diastole pre-and post-operation in patients with ASD,investigating ...
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Background In this study,we apply a novel echocardiography method,vector flow mapping(VFM),to assess the flow field changes of the right ventricle in diastole pre-and post-operation in patients with ASD,investigating its value in the evaluation of hemodynamic changes ASD *** 20 patients with secundum ASD were enrolled as patient group,while 20 healthy volunteers were chosen as controls *** characteristics of flow field pre-and post-operation were analyzed by vector,streamline and vortex modes,*** parameters including diastolic peak velocity(Vp),diastolic peak flow(Fp) and diastolic Q +(DQ +) at basal,mid and apical segments of the two groups were also recorded and compared with *** Compared with normal group,the ASD group had intensive but disordered vector and stream lines and vortexes with more area below the tricuspid leaflets in diastolic right ventricle before *** operation,the line intensity and direction consistence were recovered and the vortexes were reduced to some degree,but still different from the normal *** Vp、Fp and DQ + in each segment of right ventricle after operation were also lower;than those before ASD closure,but higher than control group(P<0.05).Conclusions The flow field hemodynamics of the right ventricle in ASD patients was recovered after operation,but still not back to normal in the short *** vector flow mapping could be used in the postoperative hemodynamic monitoring and follow-up.
Objective:To evaluate the clinical value of artificial pleural effusion as an assistant procedure of percutaneous microwave(MW) ablation treatment for hepatic malignancies under ultrasound(US) *** and methods:From Jan...
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Objective:To evaluate the clinical value of artificial pleural effusion as an assistant procedure of percutaneous microwave(MW) ablation treatment for hepatic malignancies under ultrasound(US) *** and methods:From January in 2011 to December in 2013,the clinical data of 115 patients(97 males and 18 females;mean age;57.9 ± 10.2 years old,range from 35 to 82 years old) were retrospectively analyzed,whose liver cancers would be treated by percutaneous MW ablation under US *** was necessary to assist ablation with artificial pleural effusion,as the hepatic neoplasms could not be clearly demonstrated on US or contrast enhanced US(CEUS) due to several limitations including tumors adjacent to hepatic dome and influence of pneumonectasis and liver *** patients underwent the procedure of artificial pleural effusion for the first *** procedure of artificial pleural effusion was performed after sterilization.A BD trocar needle,16 gauge in external diameter and 15 cm in length,was advanced under US guidance into patient's thoracic cavity followed rapid saline *** parameters related to feasibility,efficacy and safety of the procedure were calculated and *** successful rate,the parameter to evaluate feasibility,was defined as the percentage of patients whose thoracic cavity saline dropped into successfully after procedure in the total *** effective rate,the parameter to assess efficacy,was defined as the percentage of patients whose liver tumors were ablated according to pre-operation planning in the patients underwent a successful procedure of artificial pleural *** of the symptoms and complications related to the procedure were applied to evaluate ***:The procedure successful rate was 97.4%(112/115).During inflow,local pain was complained in 12.5%(14/112) of patients;chest discomfort was complained in 3.6%(4/112) of patients when the inflow volume was less than 500 ml;dyspnea wa
Background:Although 2-D analysis of left ventricular(LV) rotation,twist and torsion has been validated in adults and children;these measures are highly dependent on 2-D planes with limited robustness and *** aims of t...
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Background:Although 2-D analysis of left ventricular(LV) rotation,twist and torsion has been validated in adults and children;these measures are highly dependent on 2-D planes with limited robustness and *** aims of this study were to evaluate the feasibility and reproducibility of LV rotation,twist and torsion by real-time 3 D speckle-tracking echocardiography(STE)in children and to establish their normal ***:A prospective study was conducted in 347 consecutive healthy subjects(181 male/156 female,mean age 7.12 ± S.3 years,and range from birth to 18 years)using RT 3-D echocardiography(3 DE).The LV rotation,twist and torsion measurements were made off-line using new TomTec software(4 D LV-Analysis 3.0,TomTec Imaging Systems,Munich,Germany).Manual landmark selection and endocardial border editing were performed in 3 planes(apical 2-,4-,and " 3-" chamber views) and semi-automated border identification and tracking yielded LV rotation,twist and torsion ***;LV rotation,twist and torsion analysis by RT 3 D-SIT was feasible in 307 of 347 subjects(88.5%),excluding 40 subjects secondary to the low frame rates or incomplete 3-D *** is no correlation between rotational or twist and age,height,weight,BSA or HR,respectively,using canonical correlation ***,there is a negative correlation between age and LV torsion(P < 0.001).The normal ranges were defined in this cohort for rotation and twist,and for each age group for *** intraobserver and interobserver variabilities for apical and basal rotation,twist and torsion ranged from 7.3%± 3.8%to 12.3%± 8.8%and from 8.8%± 4.6%to 15.7%± 10.1%,*** correlation coefficients ranged from 0.78 to 0.89 and from 0.76 to 0.83 for intraobserver and interobserver measurements for rotation,twist and torsion,***;Analyses of LV rotation,twist and torsion by this new RT3 D STI methodology are feasible and reproducible in pediatric populat
Objective To study the effect of PHD2-shRNA genetransfectionofH9C2 cells mediated by ultrasound-targeted microbubblesdestruction(UTMD) and Polyethy-lenimine on silencing the expression of PHD2 and increasing the expre...
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Objective To study the effect of PHD2-shRNA genetransfectionofH9C2 cells mediated by ultrasound-targeted microbubblesdestruction(UTMD) and Polyethy-lenimine on silencing the expression of PHD2 and increasing the expression of HTF-1α and its downstream angiogenesis factors(VEGF,bFGF and TGF-β).Methods Culturenormoxia and anoxic H9C2 cells,and H9C2 cells are divided into four groups;normoxia H9C2 cells txansfected with EGFPplasmid(group A);normoxia H9C2 cells transfected with PHD2-shRNA plasmid(group B);anoxic H9C2 cells transfected with EGFPplasmid(groupC);anoxic H9C2 cells transfected with PHD2-shRNA plasmid(group D).Set irradiation optimal parameter which was obtained before this study(ultrasonic intensity;1.5 W/cm2,microbubbles concentration;30%,exposure time:30 sand plasmid concentration:16μg/ml) The PHD2 shRNA vector was transfected into H9C2 cells with thereporter vector H1-MCS-CMV-GFP-SV40-Neomycin that contained GFPdriven by the CMV *** 48 houra of the PHD2-shRNA and EGFP plasmid genetransfection of H9C2 cells mediated by ultrasound-targeted microbubblesdestruction and Polyethylenimine,observe green fluorescent protein with inverted flurescence *** protein expression of PHD2,HTF-1α,VEGFand bFGFwere tested by Western-blot and the mRNA expression of PHD2,HIF-1α,VEGF and bFGF were measured by *** The green fluorescent proteinwere visible in four groups after H9C2 cells were transfected for 48 *** intensity of green fluorescent protein in four groups had no difference(figure 1).RT-PCR shown that the PHD2 RNAexpression of the group B waslesser than the group A and group D lesser than group ***,HIF-1α,VEGF,bFGF and TGF-βRNA expression were precisely the *** differences were statistically significant(figure 2).The western-blot shown that the PHD2,HIF-1α,VEGF,bFGF and TGF-βprotein expression were in line with corresponding RNA expression(figure 3).Conclusion The PHD2-shRNA genetransfectionof H9C2 cells mediated by
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