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Mechanism of the antibacterial action of quinoxaline 1,4-di-N-oxides against Escherichia coli

Mechanism of the antibacterial action of quinoxaline 1,4-di-...

中国畜牧兽医学会兽医药理毒理学分会第十一届会员代表大会暨第十三次学术讨论会与中国毒理学会兽医毒理专业委员会第五次学术研讨会

作者：程古月 Bei Li Chenxi Wang Hongfei Zhang Guixia Liang Zhifei Weng Menghong Dai Yulian Wang 袁宗辉 MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products National Reference Laboratory of Veterinary Drug Residues(HZAU) and MAO Key Laboratory for Detection of Veterinary Drug ResiduesHuazhong Agricultural University

Quinoxaline 1,4-di-iV-oxides(QdNOs) are widely known as potent antibacterial agents,whereas the antibacterial mechanisms are incompletely *** the transcriptomic and proteomic profiles of Escherichia coli exposed QdNOs were integratively investigated,demonstrating that QdNOs mainly induced SOS response and oxidative ***,genes and proteins involved in bacterial metabolism,cellular structure maintenance,resistance and virulence were also changed,conferring the bacterial survival *** assays showed that reactive oxygen species were induced in the QdNO-treated bacteria,and free radical scavengers attenuated the antibacterial action of QdNOs and DNA damage,suggesting an oxidative-DNA-damage action of *** QdNO radical intermediates,probably carbon-centered and aryl-type radicals as identified by electron paramagnetic resonance were the major radicals induced by QdNOs,and the xanthine oxidase was one of the QdNO-activating *** study gives new insights into the action of QdNOs in a systematic way as well as the current knowledge of bacterial physiology under antibiotic stresses,which can be of great value in developing new antibiotic-potentiating strategies.

关键词： Quinoxaline 1,4-di-N-oxide Free radicals DNA damage QdNO-activating enzyme Antibacterial action

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Mice serum fatty acids composition correlates to hepatic fatty acids composition and NAFLD activity score induced by high-fat diet

Mice serum fatty acids composition correlates to hepatic fat...

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中国畜牧兽医学会兽医药理毒理学分会第十一届会员代表大会暨第十三次学术讨论会与中国毒理学会兽医毒理专业委员会第五次学术研讨会

作者： Xing-he Wang Chun-yan Li Xiu-ying Zhang Faculty of Basic Veterinary Science College of Veterinary MedicineNortheast Agricultural University

Background & Aim:As the leading cause of liver dysfunction all over the world,non-alcoholic fatty liver disease(NAFLD) is a clinical metabolic syndrome with fatty accumulation in hepatocytes,nevertheless,without alcohol *** recent years,morbidity of NAFLD has grown in Eastern and Western countries,and the diet composition has become an important health *** and NASH are involved in the spectrum of NAFLD,NASH is easier develop into liver fibrosis,cirrhosis,and even hepatocellular *** clinical,hver biopsy as the gold standard to assess levels of steatosis,inflammation and fibrosis in NAFLD is invasive,costly and associated with possible *** kinds of noninvasive biomarkers for evaluating NAFLD have been investigated but none performed well ***,it is still interest to discover noninvasive method to help detecting *** published data showed that quality of fatty acids(FA) accumulated in liver is associated with the degree of liver damage in *** about serum FA variation in NAFLD is *** study aims to investigate the correlation between serum FA composition and the hepatic steatosis,inflammation,hepatocellular ballooning scores and even liver FA composition in the influence of high-fat diet(HFD) in ***:ICR mice(10mice/group) were fed with HFD or control diet(CD) for 4,6 or *** were collected for pathology and NAFLD activity score(NAS) *** and liver FA composition was analyzed by gas chromatography(GC).The correlation analysis was determined with Pearson correlation ***:Induced by HFD,mice got fatty liver disease of different level without obvious fibrosis,and the liver damage level increased as the HFD feeding time went *** could be diagnosed at the 4th week of HFD feeding and NASH could be diagnosed at the 6th and 8th *** fibrosis has not occurred from the beginning to the end of the *** composition analysis in mice serum and

关键词： Pearson correlation coefficient-serum FA composition-high-fat diet-NAFLD

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栉孔扇贝和海湾扇贝线粒体16S rRNA基因片段的比较研究

栉孔扇贝和海湾扇贝线粒体16S rRNA基因片段的比较研究

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中国动物学会、中国海洋湖沼学会贝类学分会第五次代表大会暨第九次学术讨论会

作者：孔晓瑜刘亚军喻子牛宋林生教育部海水养殖研究重点实验室青岛海洋大学水产学院教育部海水养殖研究重点实验室青岛海洋大学水产学院教育部海水养殖研究重点实验室青岛海洋大学水产学院中国科学院海洋研究所

以相应引物对栉孔扇贝和海湾扇贝的线粒体16S rRNA 基因片段进行了 PCR 扩增。PCR 产物经 T 载体连接之后进行了克隆、测序,分别得到634bp 和542 bp 的碱基序列,其 A、T、G、C 含量分别为25．08％、29．65％、27．60％和17．67％(栉孔扇... 详细信息

以相应引物对栉孔扇贝和海湾扇贝的线粒体16S rRNA 基因片段进行了 PCR 扩增。PCR 产物经 T 载体连接之后进行了克隆、测序,分别得到634bp 和542 bp 的碱基序列,其 A、T、G、C 含量分别为25．08％、29．65％、27．60％和17．67％(栉孔扇贝)．25．65％、29．52％、27．31％和 17．53％(海湾扇贝)。对位排序结果表明,除两者的扩增片段长度相差92bp 外,两者的核苷酸差异数为80,同源性为68．1％;造成扩增片段长度差异的几个小区段的缺失-插入出现在扩增片段中部约130 bp 范围内。

关键词：栉孔扇贝海湾扇贝 16S rRNA 基因序列测定

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香港红树林区软体动物生态研究

香港红树林区软体动物生态研究

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中国动物学会、中国海洋湖沼学会贝类学分会第五次代表大会暨第七次学术讨论会

作者：蔡立哲黄玉山谭凤仪厦门大学环境科学研究中心香港科技大学研究中心香港城市大学生化系

1994年夏季（6月至9月）和冬季（1994年12月至1995年2月）对香港14片红树林的软体动物作了定性和定量调查。调查期间采到的软体动物已鉴定52种,其中腹足类 45种,双壳类7种。采用 Bray-Curtis 相似系数和逐步形成分类系统的方法进行聚类... 详细信息

1994年夏季（6月至9月）和冬季（1994年12月至1995年2月）对香港14片红树林的软体动物作了定性和定量调查。调查期间采到的软体动物已鉴定52种,其中腹足类 45种,双壳类7种。采用 Bray-Curtis 相似系数和逐步形成分类系统的方法进行聚类分析。基本上可分为4组断面,分别以粗束拟蟹守螺（Cerithidea djadjariensis）、奥莱彩螺（Clithon oualaniensis）、沟纹笋光螺（Terebralia sulcata）和红树拟蟹守螺（Cerithidea rhizophorarum）为第一密度优势种,不同底质的红树林区软体动物组成有显著差异。污染可能是造成沙头角红树林区软体动物多样性低的重要环境因子。盐度和潮汐制约一些软体动物的垂直分布和水平分布,纵带滩栖螺（Batillaria zonalis）和多形滩栖螺（Batillaria multiformis）在红树林区海缘高密度栖息,分别高达120/m和240个/m。在淡水注入的红树林区常有广盐性软体动物栖息,如中国绿螂（Glauconme chinensis）。

关键词：红树林软体动物生态香港

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陕西省档案学会第五次会员代表大会暨第七次档案学术讨论会在西安召开

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陕西档案 2001年第1期 16-16页

作者：王小正陕西省档案局

[本刊讯]陕西省档案学会第五次会员代表大会暨第七次档案学术讨论会于2000年12月12日至13日在西安召开.会员代表及部分论文作者共80余人参加了会议.中国档案学会发来贺信,省民政厅民间组织管理处邓文英,省社科联党组副书记冯家臻出席了... 详细信息

[本刊讯]陕西省档案学会第五次会员代表大会暨第七次档案学术讨论会于2000年12月12日至13日在西安召开.会员代表及部分论文作者共80余人参加了会议.中国档案学会发来贺信,省民政厅民间组织管理处邓文英,省社科联党组副书记冯家臻出席了会议.

关键词：陕西省档案学会会员代表大会学术讨论会民间组织论文作者会议管理处副书记中国西安社科人参民政贺信党组

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Drug delivery system based on single-wall metal-organic nanotubes

Drug delivery system based on single-wall metal-organic nano...

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中国畜牧兽医学会兽医药理毒理学分会第十一届会员代表大会暨第十三次学术讨论会与中国毒理学会兽医毒理专业委员会第五次学术研讨会

作者： Linyan Yang Cun Li Jifei Ma Tianming Jin College of Animal Science and Veterinary Medicine Tianjin Agricultural University

Cancer continues to increase with increasing age of the *** a number of situations,the malignancy of tumors is detected only at advanced stages when administration of chemotherapeutic drugs is toxic to healthy *** nanotubes(CNTs) comprised of thin sheets of benzene rings rolled up into the shape of seamless cylinders has recently attracted significant attention for use in biomedical applications[1-6].A limited number of single-wall metal-organic nanotubes(SWMONTs) have been synthesized,while a majority of reports have focused solely on structural details,SWMONTs have already shown promise in highly selective adsorptions[7].Both single-walled nanotubes(SWNT) and multi-walled nanotubes(MWNT) are being considered as a drug delivery nanocarrier[8],because they were observed to cross cell membranes[9-11]and exhibit blood circulation half-lives of the order of hours[12].The possible mechanisms proposed for CNT-drug interaction are:(a) the absorption of active components of drug within the CNT mesh,(b)non-covalent or covalent linkage of drug molecules,peptides,and nucleic acids to the exterior walls of the CNTs,and(c) the use of CNT channels as ***,we used the reported self-assembled functionalized SWMONTs to realize drug delivery of 5-Fluorouracil(5-FU),which could be assigned to the first CNT-drug interaction[13].The characterization of the material indicated the presence of drug associated with SWMONTs(5FU@SWMONTs) in a proportion of 0.33 g 5-FU per 1.0 g of *** drug demonstrated a slow release profile where 79.6%of the drug was released in PBS buffer(pH7.4) in 24 hours,while 75%in PBS buffer(pH5.7) in 24 *** cytotoxic tests were carried out against two cell lines:4T1 and *** highest lethality of cancer cells occurred at the highest concentration of 5FU@SWMONTs for the longest period of *** results demonstrate that the cytotoxicity of 5FU@SWMONTs to cancer cells was improved by the SWMONTs component.

关键词： single-wall metal-organic nanotubes drug delivery cell proliferation

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Identification of Campylobacter jejuni and determination of point mutations associated with macrolide resistance using a multiplex TaqMan MGB real-time PCR

Identification of Campylobacter jejuni and determination of ...

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中国畜牧兽医学会兽医药理毒理学分会第十一届会员代表大会暨第十三次学术讨论会与中国毒理学会兽医毒理专业委员会第五次学术研讨会

作者：郝海红刘杰 KUANG Xiuhua DAI Menghong CHENG Guyue WANG Xu PENG Dapeng HUANG Lingli AHMAD Ijaz REN Ni LIU Zhenli WANG Yulian 袁宗辉 National Reference Laboratory of Veterinary Drug Residues(HZAU) and MAO Key Laboratory for Detection of Veterinary Drug Residues Huazhong Agricultural University MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products Huazhong Agricultural University

The aim of the study was to develop a multiplex real-time PCR method to identify Campylobacter jejuni containing mutations commonly associated with macrolide resistance.A multiplex fluorescence real-time PCR assay was developed based on TaqMan minor groove binder(MGB) *** VS1-MGB probe was designed based on the VS1 gene and was used to identify Campylobacter *** 23 S rDNA-MGB probe was designed to distinguish macrolide resistance mutations in 23 S rDNA,while 57D-MGB and 74D-MGB were designed to detect resistance mutations in ribosomal protein *** specificity and accuracy of our method were identical to the conventional biochemical tests,mapA PCR,minimum inhibitory concentration(MIC) determination and DNA *** linear detection limit of the method was 003 ng genomic DNA and three colony formation unit(CFU) per *** 6 of 18 cases,the nature of Erythromycin resistance could be correctly determined from natural isolates;absence of the tested mutations was demonstrated in the remaining four resistant ***,a multiplex TaqMan MGB real-time PCR assay with high specificity and accuracy was developed to simultaneously identify *** and detect the gene mutations associated with macrolide *** multiplex method can potentially simplify the identification of Camp,jejuni and determine macrolide resistance due to mutations in 23 S rDNA or ribosomal protein *** method has a potential for application in different research areas and molecular surveillance.

关键词： C.jejuni macrolide resistance MGB point mutation real-time PCR.

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米尔贝肟吡喹酮片在猫的靶动物安全性试验

米尔贝肟吡喹酮片在猫的靶动物安全性试验

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中国畜牧兽医学会兽医药理毒理学分会第十一届会员代表大会暨第十三次学术讨论会与中国毒理学会兽医毒理专业委员会第五次学术研讨会

试验将32只成年健康猫随机分成4组(公母各半),采用多剂量水平给药进行靶动物安全性研究,其中受试药物设最大推荐剂量(每kg体重4mg米尔贝肟和10mg吡喹酮)、3倍最大推荐剂量(每kg体重12mg米尔贝肟和30mg吡喹酮)和5倍最大推荐剂量(每kg体重... 详细信息

试验将32只成年健康猫随机分成4组(公母各半),采用多剂量水平给药进行靶动物安全性研究,其中受试药物设最大推荐剂量(每kg体重4mg米尔贝肟和10mg吡喹酮)、3倍最大推荐剂量(每kg体重12mg米尔贝肟和30mg吡喹酮)和5倍最大推荐剂量(每kg体重20mg米尔贝肟和50mg吡喹酮)三个剂量组;另设一空白对照组,给予不含任何活性成分的空白片剂。通过一般临床观察、各组动物体重、血液学和血液生化学参数等指标及组织病理学检测或观察,评价米尔贝肟吡喹酮片在临床用药的安全性。结果表明,试验期间各组猫均未死亡且健康良好,期间临床表现正常,5倍最大推荐剂量组剖检动物各组织器官未发现病理变化。在血液生理指标及生化指标方面,推荐剂量组、3倍推荐剂量组、5倍最大推荐剂量组与空白对照组相比,各指标并无显著差异。试验结果说明,浙江海正药业股份有限公司生产的米尔贝肟吡喹酮片在推荐剂量下使用在靶动物猫上具有较高的安全性。

关键词：猫米尔贝肟吡喹酮片靶动物安全性

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西施舌的形态学研究

西施舌的形态学研究

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中国动物学会、中国海洋湖沼学会贝类学分会第四次代表大会暨第五次学术讨论会

作者：黄一鸣王方平福建师范大学生物系福建师范大学生物系

本文对西施舌的贝壳、外套膜、内脏团、足部、水管、肌肉系统、消化系统、呼吸系统、循环系统、排泄系统、神经系统和生殖系统等进行了解剖,并对动脉球和嗅检器等器官系统的构造进行了较详细的研究。西施舌 Mactra antiquata(spengler)... 详细信息

本文对西施舌的贝壳、外套膜、内脏团、足部、水管、肌肉系统、消化系统、呼吸系统、循环系统、排泄系统、神经系统和生殖系统等进行了解剖,并对动脉球和嗅检器等器官系统的构造进行了较详细的研究。西施舌 Mactra antiquata(spengler)俗称“海蚌”,属瓣鳃纲 Lamellihranchia 异齿亚纲 Her- erodonta 帘蛤目 Veneroida 蛤蜊科 Mactridae。西施舌为广温性种,分布于太平洋西部、印度半岛、日本、朝鲜及我国沿海,福建省尤以长乐县盛产．西施舌栖息在潮间带下区和潮下带的沙滩、沙泥滩中,以底栖硅藻为主食,西施舌以其肉嫩味美而闻名,为名贵的海珍品。有关西施舌的研究报导：张玺、齐钟彦等(1960—1962)曾报导了西施舌的外部形态及在我国海区的分布情况;陈文龙、刘德经等(1966)对西施舌的人工育苗试验作了初步报导;黄一鸣、林秀瑛等(1983)报导了西施舌生殖腺的季节性变化。至于西施舌的形态研究,迄今尚未见报导。本文对西施舌的形态结构和组织学进行研究,为教学及进一步发展西施舌的人工养殖提供参考。

关键词：西施舌组织学形态学

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三种微藻对波部东风螺幼虫存活、生长及变态的影响

三种微藻对波部东风螺幼虫存活、生长及变态的影响

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中国动物学会、中国海洋湖沼学会贝类学分会第五次代表大会暨第九次学术讨论会

作者：郑怀平柯才焕周时强李复雪盐城工学院海洋工程系厦门大学海洋学系厦门大学海洋学系厦门大学海洋学系

分别用不同浓度的湛江叉鞭金藻、亚心形扁藻及盐生杜氏藻投喂波部东风螺面盘幼虫来研究藻类及其浓度对其幼虫生长发育的影响。结果表明幼虫对三种藻类均能够摄食,摄食金藻和扁藻的幼虫都能够较快地生长,最大生长速度分别可达到35．72μm... 详细信息

分别用不同浓度的湛江叉鞭金藻、亚心形扁藻及盐生杜氏藻投喂波部东风螺面盘幼虫来研究藻类及其浓度对其幼虫生长发育的影响。结果表明幼虫对三种藻类均能够摄食,摄食金藻和扁藻的幼虫都能够较快地生长,最大生长速度分别可达到35．72μm·d(25×10～4cells·mL)和 36．94μm·d(9×10～4cells·mL),但摄食盐藻的幼虫却生长的比较慢。投喂盐藻的幼虫均在没有变态之前就全部死亡,而投喂金藻和扁藻的幼虫组,除金藻浓度1×10～4cells·mL组外,其他各组都有变态个体出现。但投喂扁藻的幼虫组,幼虫的存活率和变态率均不高,二者最高的只有 23．5％和19．5％,而投喂金藻的幼虫组,却可达到51％和49．5％。三种藻类相比较,湛江叉鞭金藻是波部东风螺面盘幼虫的最佳饵料,且适宜浓度为（15～25）×10～4cells·mL。

关键词：微藻生长波部东风螺存活生长变态

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