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To construct a dense genetic map and to map QTLs controlling some important characters in oilseed rape

To construct a dense genetic map and to map QTLs controlling...

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湖北省遗传学会第七次代表大会暨学术讨论会

作者： Dan Qiu, Jiaqin Shi, Yan Long and Jinling Meng National Key Laboratory of Crop Genetic Improvement,- Huazhong Agricultural University, Wuhan 430070, China.

A total of 202 doubled haploid （DH） lines was developed from the F between Tapidor, a European modern double low winter-typed cultivar, and Ningyou7, Chinese old double high cultivar, by microspore culture. The DH population was grown in two places under different environment for 2 years. 12 yield-related and quality-related QTLs characteristics had been investigated in two growing site in 2003. It should be noticed that the F1 shown strong heterosis on most of seed yield related characteristics, and the seed yield in both sites. A linkage map including 68 SSR marker loci was constructed and QTL analysis was based on this linkage map with the software WinQTLCart 2.0（Zhao-Bang Zeng）. QTL for seed oil content, flowering time, seed weight per plant and seed weight （g/1000seeds） both in two growing site were identified. The further research will focus on increasing the number of markers to 200 on the map with 80 RFLP makers and other SSR markers. Hundreds of SNP makers coming from other laboratories will integrate into the map. QTLs involving 12 characteristics will be mapped in the new genetic map. Correlation analyses of these traits will be studied and the same trait under the different environment will be compared. The genetic analysis of yield-related QTLs and the seed quality-related QTLs under different environments would provide useful information for the oilseed breeding.

关键词： Rapeseed SSR QTL mapping Seed Yield-related QTLs Seed Quality-related QTLs

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药用野生稻与栽培稻第5染色体比较核型分析及抗性基因Xa-5的比较物理定位

药用野生稻与栽培稻第5染色体比较核型分析及抗性基因Xa-5的比较...

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湖北省遗传学会第七次代表大会暨学术讨论会

作者：覃瑞宋运淳刘学群蔡健相宋发军吴士筠何光存中南民族大学化学与生命科学学院生物技术国家民委重点实验室武汉大学生命科学学院教育部发育生物学重点实验室中南民族大学化学与生命科学学院生物技术国家民委重点实验室韩国全北大学基础科学部分子生物学实验室中南民族大学化学与生命科学学院生物技术国家民委重点实验室中南民族大学化学与生命科学学院生物技术国家民委重点实验室武汉大学生命科学学院教育部发育生物学重点实验室

药用野生稻是栽培稻的重要种质资源,从药用野生稻中发掘出的抗病基因具有抗性强、抗谱广、抗性稳定的特点,因而对野生稻资源的研究正日益受到重视。本研究通过水稻细菌人工染色体(BAC)克隆的荧光原位杂交(BAC-FISH)进行了抗病虫基因Xa-... 详细信息

药用野生稻是栽培稻的重要种质资源,从药用野生稻中发掘出的抗病基因具有抗性强、抗谱广、抗性稳定的特点,因而对野生稻资源的研究正日益受到重视。本研究通过水稻细菌人工染色体(BAC)克隆的荧光原位杂交(BAC-FISH)进行了抗病虫基因Xa-5在药用野生稻和栽培稻的染色体比较物理作图,并通过比较作图分析了药用野生稻第5染色体的核型。选用栽培稻RFLP标记RG556及其筛选的BAC克隆4484作探针,通过荧光原位杂交确定了药用野生稻第5染色体的有丝分裂核型。它们的RL和AR值相应地为5.20±0.05,和1.22±0.04,利用原位杂交以遗传组成为依据的染色体核型分析是一种新的尝试,国内外尚属首次。由于RG556和4484与抗性基因Xa-5紧密连锁,从而确定该基因分别位于栽培稻和药用野生稻的短臂和长臂上,我们推测这可能与倒位有关,但这种倒位属含着丝粒两边的臂间倒位,而不是臂内倒位。本研究的目的是为了使栽培稻与药用野生稻含相同或类似遗传组成的染色体编号基本上达到一致,进一步阐明它们之间的亲缘关系与进化历程,提高栽培稻这一模式植物的研究成果在近缘种属中的通用性,加速野生稻遗传资源的开发利用,促进稻属不同种之间,甚至禾本科不同属之间大遗传体系的建立。

关键词： BAC-FISH 核型分析 O.officinalis 物理定位 Xa-5

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Zebrafish (Danio rerio) as a model for the study of infectious reaction against Spring viraemia of carp virus-A1

Zebrafish (Danio rerio) as a model for the study of infectio...

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中国水产学会鱼病专业委员会第七次会员代表大会暨学术讨论会

作者： Dan Xu SiewHong Lam ChoongYong Ung MyaMyintzu Hlaing QiWei Qin Zhi Yuan Gong State Key Laboratory of Biocontrol Sun Yat-sen University Guangzhou 510275 PR China Department of Biological Sciences National University of Singapore 14 Science Drive 410 King Ridge CrescentSingapore 119260 Singapore Laboratory of Marine Biology South China Sea Institute of Oceanology Chinese Academy of Sciences 164 West Xingang Road Guangzhou 510301 PR China

Spring viraemia of carp (SVC) is a viral disease that causes outbreaks in common carp (Cyprinus carpio) and several other freshwater *** is getting more attention as an infection model and some experimental infections have been conducted with different bacteria and ***, zebrafish have been shown to be susceptible to infection with the rhabdovirus spring viraemia of carp virus (SVCV).In order to investigate viral pathogenesis process in liver and capture liver inflammation expression signature/***-A 1 was infected with zebrafish and captured at the 24hr, 48hr and 96hr time *** zebrfish livers were separated from the body for microarray and fixed in buffered formalin for different staining: Hematoxylin and Eosin (H&E) staining and other Immunohistochemistry staining (E-cadherin and Cytokeratin-18), ApopTag staining and Periodic acid-Schiff (PAS) staining.

关键词： SVCV-A1 Zebrfish Staining Microarray Pathway

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A new chromosome fluorescence banding techniquecombining DAPI staining with image analysis in plants

A new chromosome fluorescence banding techniquecombining DAP...

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湖北省遗传学会第七次代表大会暨学术讨论会

作者： Jingyu Liu~(1.2) Chaowen She~1 Zhongli Hu~1 Zhiyong Xiong~1 Lihua Liu~1 Yunchun Song~1 (1 The Key Laboratory of the Ministry of Education of Developmental Biology, WuhanUniyersity, Wuhan, P. R. China, 430072 2. College of Life Science, Henan University, Kaifeng, P. R. China, 475001)

In this study, a new chromosome fluorescence banding technique was developed in plants. The technique combined DAPI staining with software analysis including three-dimensional imaging after deconvolution. Clear multiple and adjacent DAPI bands like G-bands were obtained in the tested species including Hordeum vulgare L., Oryza officinalis Wall & Watt, Triticum aestivum L., Lilium brownii Brown, and Vicia faba L. by this technique. During mitotic metaphase number of the bands for their haploid genomes was about 185, 141, 309, 456 and 194, respectively. The reproducibility analysis demonstrated that banding patterns within a species were stable at the same mitotic stage and they could be used for identifying specific chromosomes and chromosome regions. The band number was fluctuated, and the earlier the mitotic stage, the more the bands. The technique enables genes to map onto specific band regions of the chromosomes by only one FISH step with no chemical banding treatments. In this study, 45S and 5S rDNA of some tested species were located on the specific band regions of the specific chromosomes and they all were positioned at the interbands with the new technique. Because of no chemical banding treatment, the banding patterns displayed by the technique should basically reflect the natural conformational features of chromatin. Thus it could be expected that this technique should be suitable to all the eukaryotes and would have widespread utility in chromosomal structure analysis and physical mapping of genes.

关键词： Plant chromosomes DAPI banding Three-dimensional imaging Deconvolution FISH

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Microcalorimetric Study on the Interactions between Escherichia coli B and itsT4 Phage in the Early Phase of Infection

Microcalorimetric Study on the Interactions between Escheric...

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湖北省遗传学会第七次代表大会暨学术讨论会

作者： Liu Guo-Sheng~(1,2), Liu Yi~3, Li Mei-Ju~1, Chen Xiang-Dong~(1*), Zhu Jun-Cheng~3, Shen Ping~1 1 College of Life Sciences, Wuhan University, Wuhan 430072, P. R. China 2 College of Life Sciences, Henan Normal University, Xinxiang 453002, P. R. China 3 College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, P. R. China

In a restricted medium the interactions between T4 phage and resting cells of Escherichia coli B in the early phase of phage infection were measured using LKB-2277 bioactivity monitor. Non-growth metabolism of E. coli B-T4 phage system possesses the same characteristic as E. coli B: output power（P） has a. linear correlation with cell concentration with the equation P=1.68369×10 C+1.86 for E. coli B, and the equation P=2.23787×10C+3.595 for T4 infected E. coli B. The output power of single cell（SCP） is restrained by cell density, indicating that there is a "space effect" for resting metabolism, At one of the conditions, non-infection state or infection state, the total quantity of heat（Q） does not change significantly with the cell concentration change. But P, SCP and Q of infected cells, are all higher than that of non-infected cells. It is confirmed that T4 phage infection can enhance metabolism of host cells. Heat output power of single infected cell（ISCP） exceeds that of uninfected cell by 1.23 pW to 2.03 pW at various cell concentration. The metabolic activity of infected cell is enhanced by 44.57% to 61.70% in comparison to uninfected cell.

关键词： Microcalorimetry, Phage-host Interaction, Resting cell, Non-growth metabolism, Metabolic activity

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A dominant gdcP specific marker derived from Moricandia nitens assistant for introducing the C3-C4 character from *** into Brassica crops

A dominant gdcP specific marker derived from Moricandia nite...

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湖北省遗传学会第七次代表大会暨学术讨论会

作者： C. Zhang, G. Xu, R. Huang, C. Chen and J. Meng~1 National Key Lab of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China

Differential localization of decarboxylation and carboxylation in association with Kranz anatomy distinguish species with C or C-C intermediate photosynthesis from species with C photosynthesis. Moricandia nitens has a C-C4 intermediate photosynthetic mechanism and yet is closely related to C Brassica species. In order to introduce the C-C character from M. nitens into Brassica crops, sesquitetraploids （MACC） were synthesized by crossing Brassica napus （AACC） and somatic hybrid （M. nitens + B. oleracea, MMCC）. 2,966 bp and 3,254 bp homologous fragments of P-protein gene of glycine decarboxylase （gdcP） were isolated from M. nitens and B. napus, respectively, to develop a PCR-based marker for assisted selection. RT-PCR of the partial encoding regions of both gdcPs indicated that the mRNAs were expressed in the expanded leaf. The characterization of high divergence, but with large conserved island in the regulatory region of the two C species, implies that they may play an important role in maintaining the C photosynthetic pathway. Thereafter, a dominant gene specific marker was developed within the highly divergent region for subsequent introduction of C-C photosynthesis from Moricandia nitens into Brassica crops.

关键词： A dominant gdcP specific marker derived from Moricandia nitens assistant for introducing the C3-C4 character from M.nitens into Brassica crops

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一株高产铁载体假单胞菌的分离鉴定和生理特性的研究

一株高产铁载体假单胞菌的分离鉴定和生理特性的研究

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湖北省遗传学会第七次代表大会暨学术讨论会

作者：陈绍兴赵翔谢志雄沈萍武汉大学生命科学学院武汉大学生命科学学院武汉大学生命科学学院武汉大学生命科学学院

铁离子在细胞内是一种不可缺少的元素,可以作为电子传递链的电子受体,以及很多氧化还原酶的辅基。虽然铁元素在地壳中的含量占第四位,但是游离的铁离子在自然环境中极其微量(10M),远远低于生物体生长的要求(10M)。大部分微生物,真菌（... 详细信息

铁离子在细胞内是一种不可缺少的元素,可以作为电子传递链的电子受体,以及很多氧化还原酶的辅基。虽然铁元素在地壳中的含量占第四位,但是游离的铁离子在自然环境中极其微量(10M),远远低于生物体生长的要求(10M)。大部分微生物,真菌（如大豆根瘤菌）,以及一些植物,包括一些农作物;如禾本科的水稻、玉米、小麦等,均能分泌一种或多种类型的铁载体。铁载体的最大特性就是能够和铁离子有很强的螯合能力,细胞通过细胞膜上面的铁载体的受体蛋白,将螯合有铁离子的铁载体通过消耗ATP的过程运输到细胞内,从而达到从环境中富集铁离子的作用。铁载体是一种活性多肽,分子量一般在500-1500Da左右。我们用CAS常用的检测铁载体的方法从武汉东湖的浅表层水体（50cm）中分离到能够产铁载体的菌株,在相同培养条件下,其中有一株菌的铁载体产量是其它的细菌的2-5倍。用Biolog细菌快速鉴定板进行初步鉴定为假单胞菌,克隆测序了其16SrRNA基因,在NCBI数据库进行序列比较分析,结果显示我们分离的这株菌属于假单胞菌属。在这基础上我们从中国典型培养物保藏中心获得了假单胞菌属的7个种的菌株和我们自己分离的菌株进行了产铁载体的比较,结果显示我们分离的这一菌株其产量明显高于我们所选的那7个株菌。由此我认为我们分离到的是一株铁载体的高产菌株。我们进一步作了它的生理方面的研究:a.对大多数的抗生素均存在较大的抗性,如氨苄青霉素,氯霉素等;b.发现最适生长的pH在7.0左右;c.最适生长的温度在26-30℃;d.通气越好,越有利于产铁载体;e.在外加铁离子的情况下大大降低了铁载体的产量,在加入适当的铁离子的螯合剂的情况下铁载体的产量明显增加;f铁载体一般在对数生长后期开始大量分泌,进入稳定期后期达到最大;g.针对不同的氮源和炭源,我们得到了一种相对优化的培养基,优化的培养基上,铁载体的产量比原来的提高了近五倍。

关键词：铁载体假单胞菌生理特性

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细胞质雄性不育水稻线粒体基因组的RFLP分析

细胞质雄性不育水稻线粒体基因组的RFLP分析

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湖北省遗传学会第七次代表大会暨学术讨论会

作者：黄威汪莉易平谭学林张雪梅张再君李阳生朱英国武汉大学植物发育生物学教育部重点实验室武汉大学植物发育生物学教育部重点实验室武汉大学植物发育生物学教育部重点实验室云南农业大学水稻研究所云南农业大学水稻研究所武汉大学植物发育生物学教育部重点实验室武汉大学植物发育生物学教育部重点实验室武汉大学植物发育生物学教育部重点实验室

水稻细胞质雄性不育按照其不育性的遗传特点可以分成两种类型:一种是配子体细胞质雄性不育,包括红莲型、包台型和滇一型;另一种是孢子体细胞质雄性不育,包括马协型、野败型、冈型、K型、D型和Ⅱ型。利用RFLP技术,比较研究了在农业生产... 详细信息

水稻细胞质雄性不育按照其不育性的遗传特点可以分成两种类型:一种是配子体细胞质雄性不育,包括红莲型、包台型和滇一型;另一种是孢子体细胞质雄性不育,包括马协型、野败型、冈型、K型、D型和Ⅱ型。利用RFLP技术,比较研究了在农业生产上广泛应用的九种细胞质雄性不育体系的线粒体基因组,结果表明: (1) 九种雄性不育细胞质的遗传相似性变化范围为0.615-1.000。所有配子体雄性不育细胞质的遗传相似性变化范围为0.643-1.000,其中QXA,DY1A和YM15A三种细胞质的遗传相似性为1.000。所有孢子体雄性不育细胞质的遗传相似性为1。000。(2) 在配子体细胞质雄性不育水稻申,用三个探针(atpA,atp9+nad6,cox2)与两种内切酶(HindⅢ,BamH Ⅰ)的组合未发现不育系与保持系之间的多态性,但在探针atp6,cob,had2的杂交带型中找到了不育系和保持系之间的一些差异。YTA和YTB在五个探针-内切酶组合(atp6/HindⅢ,cob/HindⅢ,atp6/BamH Ⅰ,cob/BamH Ⅰ,nad2/BamH Ⅰ)中存在差异。三种细胞质(QX,SJ,DY1)的不育系和保持系之间的差异是相同的,都出现在atp6/HindⅢ,atp6/BamH Ⅰ,cob/BamH Ⅰ三个组合中。YM15A和YM15B在四个组合(atp6/HindⅢ,atp6/BamH Ⅰ,cob/BamH Ⅰ,nad2/BamH Ⅰ)中存在差异。LYA和LYB的差异出现在cob/HindⅢ,cob/BamH Ⅰ,nad2/BamH Ⅰ这三个组合中。(3) 在孢子体细胞质雄性不育水稻中,所有不育系的带型是一样的,所有保持系的带型也是一样的。不育系和保持系的差异出现在atp6/HindⅢ,cob/HindⅢ, atp6/EcoR Ⅰ,cob/EcoR Ⅰ,cox1/EcoR Ⅰ,atp6/BamH Ⅰ,cob/BamH Ⅰ,cox1/BamH Ⅰ,cox2/BamH Ⅰ九个组合中。这些结果在分子水平上揭示了九种雄性不育细胞质的线粒体基因组存在结构多样性,并为其细胞质雄性不育分子机理的研究打下了基础。

关键词：细胞质雄性不育线粒体基因组 RFLP 水稻

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Genetic Characterization of Boron Efficiency in Rape(Brassica napus L.)

Genetic Characterization of Boron Efficiency in Rape(Brassic...

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湖北省遗传学会第七次代表大会暨学术讨论会

作者： Lei Shi~(1,2), Fangsen Xu~(1,2), Jia Liu~1, Fuzhao Nian~2, Jianwei Lu~2, Jinling Meng~1 & Yunhua Wang~2 1 National Key Laboratory Of Crop Genetic Improvement, National Center of Crop Molecular Breeding, Huazhong Agriculture University., Wuhan 430070, P.R. China 2 Laboratory of Microelement, College of Resources and Environment, Huazhong Agriculture University., Wuhan 430070, P.R. China

Soil B deficiency always causes the decrease of the rapeseed （Brassica napus L） yield and quality. Genetic variation exists for B-efficiency among different Brassica napus germplasms. Field experiments were conducted to study the genetic control of boron efficiency in oil seed rape by boron efficient coefficient （BEC, ratio of the seed yield at the critical boron level to that at the boron sufficient level） with 657 F lines, which was derived from a cross between a high B-efficiency cultivar, 'Qingyou 10' and a low B-efficiency cultivar, 'Bakow'. Results showed that the parents showed significant differences in both seed yield under boron deficiency treatment and BEC. The distribution of the seed yield of the 657 F lines showed normal distribution （mean=81.88g;SD=20.56） under supplied sufficient boron. Boron deficiency decreased the seed yield of Brassica napus significantly and the distribution of BECs of the F lines showed a bimodal distribution. The 657 F lines were grouped into high boron efficiency lines and low boron efficiency lines depended on the bimodal distribution of BECs. The ratio of high boron efficiency to low boron efficiency lines fitted the expected ratio of 3:1, indicating that one major gene controlled the B-efficiency trait, which further confirmed the results we obtained from a F population with 183 individuals （Xu, 2001）. 127 F lines selected randomly in the population, whose distributions of the BEC and seed yield under 2 treatments were similar to the population, could be used to narrow down the target region for fine-mapping the high boron efficiency gene. Abbreviation: B - boron;HWSB - hot water soluble boron;BEC - boron efficiency coefficient;*** - Brassica napus L.

关键词： B-boron, deficiency, BEC, high boron efficiency Brassica napus L.

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Expression profiles of the hybrid and parents at three stages of panicle development as revealed by cDNA microarray analysis

Expression profiles of the hybrid and parents at three stage...

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湖北省遗传学会第七次代表大会暨学术讨论会

作者： Yi Huang, Dejun Yuan, Jianwei Zhang, Xianghua Li, Caiguo Xu, Shiping Wang and Qifa Zhang National Key Laboratory of Crop Improvement, Huazhong Agricultural University, Wuhan 430070, China

There have been intensive genetic studies heterosis based on molecular marker technology and high-density linkage maps, which have provided data for critical assessment of long-debating hypotheses concerning the genetic basis of heterosis. The current study was undertaken to compare the genes that were differentially expressed in an elite hybrid and its parents during young panicle development, as the first step to understand heterosis at the molecular level. In this study, a high-density cDNA microarray consisting of 9216 unique rice gene sequences derived from a normalized cDNA library made up of 14 different tissues was used to profile global gene expression patterns. Young panicles at the secondary-branch primordia stage, pistil-stamen primordia stage and pollen progenitor cell stage of development were harvested from Zhenshan 97, Minghui63 and their hybrid Shanyou 63, the most widely cultivated hybrid in China. The first cDNA strands of hybrid and both parents were labeled with Cy5-dye;hybridization was done on 3 microarrays at a time, with two independent replications each. Ratios of hybridization signals of the hybrid to each of the parents were calculated. To assess the error variation from chip to chip, we performed two independent hybridizations with the targets of Zhenshan97 at pollen progenitor cell stage onto two different chips simultaneously. The normalized ratios of slidel/slide2 were used to represent the gene expression levels in the experimental samples. We chose 1.65-fold as the threshold of sigrfificance. The criteria were applied to the mean values of the 2 data sets produced by replicating hybridizations. A gene was regarded as upor down- regulated only if the ratios obtained in both of the replicates were larger than 1.65 or smaller than 0.60.

关键词： profile microarray Expression profiles of the hybrid and parents at three stages of panicle development as revealed by cDNA microarray analysis

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