The susceptibility of eight marine fish species cultured in South China were tested for infection by the parasitic ciliate, Cryptocaryon irritans, via a challenge examination and an immobilization *** species of fish ...
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The susceptibility of eight marine fish species cultured in South China were tested for infection by the parasitic ciliate, Cryptocaryon irritans, via a challenge examination and an immobilization *** species of fish (representing six different families) that we investigated were infected by *** except the rabbitfish (Siganus oramin), which displayed resistance to *** *** infection intensity of rabbitfish (0.92±0.969, p<0.05) was significantly lower while the immobilization titres of rabbitfish serum were significantly higher (44.51±22.982 p<0.05) than the other seven species of fish.
Studies were conducted to determine the cause of the high level mortalities of cultured half-smooth tongue sole (Cynoglossus semilaevis).Gross signs of disease included loss of appetite, erratic swimming, and haemorrh...
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Studies were conducted to determine the cause of the high level mortalities of cultured half-smooth tongue sole (Cynoglossus semilaevis).Gross signs of disease included loss of appetite, erratic swimming, and haemorrhages at the head, opercula and base of fin, rot of dorsal fin, swelling of the abdomen filled with ascitic fluid, and exposure of intestine tube outside of *** examination of the liver showed focal areas of necrosis and extensive *** pure dense cultures were obtained from liver, kidney, spleen tissues, and strong pathogenicity of the isolates to tongue sole was confirmed.
A marine fish cell line derived from the kidney of red spotted grouper (Epinephelus akaara) named EAGK was established and characterized, this cell line has been subcultured more than 80 *** cells multiplied well in L...
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A marine fish cell line derived from the kidney of red spotted grouper (Epinephelus akaara) named EAGK was established and characterized, this cell line has been subcultured more than 80 *** cells multiplied well in Leibovitz's L-15 medium containing 10% fatal bovine serum at temperatures between 25~30℃.The EAGK cell line has a modal diploid chromosome number of *** expression of vimentin, desmin and fibronectin can be tested in EAGK cells while cytokeratin can't, with immunofluorescence experiment using four antibodies respectively.
Owing to its specific binding to carbohydrates, lectins play a crucial role in pathogen recognition and clearance in vertebrate ***, only two types of collectins had been isolated from bony fish: mannan-binding iectin...
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Owing to its specific binding to carbohydrates, lectins play a crucial role in pathogen recognition and clearance in vertebrate ***, only two types of collectins had been isolated from bony fish: mannan-binding iectin (MBL) and galactose-binding lectin (GalBL).In this study, the gene sequence of a novel collectin (Designed EALec1) from red-spotted grouper Epinephelus akaara has been *** genomic structure of EALec1 and alignment of Carbohydrate recognition domain(CRD) sequence amcng three collectins implied that EALec1, is a new type collectin derived from MBL.
作者:
Jinggeng ZhouDan XuJingguang WeiQiwei QinState Key Laboratory of Biocontrol
School of Life Science Sun Yat-sen University 135 West Xingang Road Guangzhou 510275 Key Laboratory of Marine Bio-resourses Sustainable Utilization South China Sea Institute of Oceanology The Chinese Academy of Sciences 164 West Xingang Road Guangzhou 510301
Hepcidin gene is widely expressed in various fish, suggesting that this antimicrobial peptide is a very important component in the innate immune *** (Epinephelus tauvina) is one of the important economic species of ma...
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Hepcidin gene is widely expressed in various fish, suggesting that this antimicrobial peptide is a very important component in the innate immune *** (Epinephelus tauvina) is one of the important economic species of marine-cultured fish but knowledge of its innate immune mechanism is *** this study, we found two different types (designated Hep-ET1 and Hep-ET2) of hepcidin cDNA in the *** consist of an open reading frame of 267and 263 bases encoding 88 and 87 amino acids respectively and both have a conserved sequence in common with other known hepcidins.
Motility is thought to contribute to the virulence of many pathogenic *** order to understand the role of motility of Vibrio harveyi in virulence, the mini-Tn10/Kan transposoninduced mutants with deficient motility in...
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Motility is thought to contribute to the virulence of many pathogenic *** order to understand the role of motility of Vibrio harveyi in virulence, the mini-Tn10/Kan transposoninduced mutants with deficient motility in semisoft medium were constructed, screened and ***, a mutagenesis clone bank of *** TS-628 was obtained by cell conjugation between the donor *** Sm10 (pLOFKm) containing the mini-Tn10Kan and the recipient *** TS-628 by filter *** seventeen non-motile mutants were screened from the mutans bank by phenotypical characterization in semisoft medium, two of which were finally confirmed to be mini-Tn10/Kan transposon-induced motility mutants after identified by PCR and Southern *** the growth rate, ability of chemotaxis and biofilm formation of the mutations and the wild type strain were assessed.
In this paper, we report the molecular cloning of a novel stefin analogue from the spleen of large yellow croaker Pseudosciaena crocea (Lycstefin).The open reading frame (ORF) of 297 nucleotides (nt) of Lycstefin enco...
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In this paper, we report the molecular cloning of a novel stefin analogue from the spleen of large yellow croaker Pseudosciaena crocea (Lycstefin).The open reading frame (ORF) of 297 nucleotides (nt) of Lycstefin encodes a protein of 99 amino acids (aa) with a putative molecular weight of 1 l kDa, in which no signal peptide and potential N-glycoslation site are *** deduced Lycstefin possesses the structural features of the mammalian stefins, including two conserved motifs known to interact with the active sites of family C 1 cysteine peptidases: one glycine in the N-terminal region (G6) and GIn-Xaa-Val-Xaa-Gly motif (Q48LVAG52).It shares 32-47.5% aa sequence identity to the sequences found in mammals and other fish species and is rich in cysteine residues (seven cysteines).
Soft-shelled turtle iridovirus (STIV) is the causative agent of serious diseases in the farmed soft-shelled turtle (Trionyx sinensis).Here we determined the complete nucleotide sequence of STIV *** STIV genome is 1058...
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Soft-shelled turtle iridovirus (STIV) is the causative agent of serious diseases in the farmed soft-shelled turtle (Trionyx sinensis).Here we determined the complete nucleotide sequence of STIV *** STIV genome is 105890 bp in length with a base composition of 55.1% G+*** assisted analysis revealed that STIV contained 105 potential open reading frames (ORFs) which encode polypeptides ranging from 40 to 1,294 amino acids and 20 MicroRNA candidates.
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