Edwardsiella tarda is an important bacterial pathogen to cultured *** of △aroA, △aroA△esaC, △aroA△evpH and △aroA△esrB were constructed in *** wild-type LSE40 by approach of in-frame ***50 (50% lethal dose) of t...
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Edwardsiella tarda is an important bacterial pathogen to cultured *** of △aroA, △aroA△esaC, △aroA△evpH and △aroA△esrB were constructed in *** wild-type LSE40 by approach of in-frame ***50 (50% lethal dose) of these four mutants was determined in Blue gourami (Trichogaster trichopterus).Results indicated that the virulence of these mutant decreased by 13、 159、 1350 and 10000 times, *** evaluation the immune protection, the four mutants were injected muscularly (i.m.) into the Blue gourami fish at the dose of 2.15× 105 cfu/fish、 2.00× 106 cfu/fish、 1.73× 106 cfu/fish and 2.09× 107 cfu/*** 30 days of injection, the relative percent survival (RPS) of immunized fish was determined by *** with 1.91×104 cfu/fish of the wild-type *** indicated that fish injected with these four mutant show RPS values of 0, 54.55%, 0 and 90.91%, *** suggest that △aroA△esrB mutant could be used as a potential attenuated vaccine against Edwardsiellosis.
A total of 780 Megalobrama amblycephal were divided into two groups *** control group was only fed with basal diet; the test groups fed with basal diet added with 0.1% anthraquinone extract, *** 70d, fish were infecte...
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A total of 780 Megalobrama amblycephal were divided into two groups *** control group was only fed with basal diet; the test groups fed with basal diet added with 0.1% anthraquinone extract, *** 70d, fish were infected *** fish growth, blood cortisol, total protein, glucose, lysozyme and hepatic antioxidant indices, HSP70 mRNA level at 6h, 12h, 24h, 48h after *** infection were *** results showed that the growth gain, specific growth rate, blood lysozyme activity and hepatic superoxide dismutase activities in treated fish increased whereas the feed conversion rate, mortality rate reduced in treated fish before *** infection.
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