Transgenic tomato plants with tobacco osmotin gene and bean basic chitinase gene together with the NPTⅡmarker gene were applied in this experiment. A kanamycin spraying test was carried out on 3 successive generatio...
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Transgenic tomato plants with tobacco osmotin gene and bean basic chitinase gene together with the NPTⅡmarker gene were applied in this experiment. A kanamycin spraying test was carried out on 3 successive generations （T, T, T） of several transgenic lines with varied T-DNA copy number detected by. Southern blot on To generation. Three-week transgenic seedlings as well as the non-transformed control growing in tray in greenhouse or growing on open seedling bed were sprayed from above with 100 mg/L
Hybrid sterility frequently occurs in crosses between indica and japonica subspecies of Asian cultivated rice. In this study, we investigated the cytological processes involved in formation, and development of male an...
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Hybrid sterility frequently occurs in crosses between indica and japonica subspecies of Asian cultivated rice. In this study, we investigated the cytological processes involved in formation, and development of male and female gametes as well as their interactions in fertilization, using an indica/japonica hybrid in comparison with an indica/indica hybrid. It was found that more than 50% of the microspores generated in the indica/japonica hybrid could not develop into functional
Ca plays an essential role in the competence development of Escherichia coli cells. Using the potassium pyroantimonate method, we compared the Ca localization in competent and non competent E. coli cells. We found tha...
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Ca plays an essential role in the competence development of Escherichia coli cells. Using the potassium pyroantimonate method, we compared the Ca localization in competent and non competent E. coli cells. We found that two types of calcium pyroantimonate precipitates, round and acicular, existed on the surface of competent cells, but were not detected on non-competent cells, Though a few round or amorphous precipitates were found in the cytosol, there was no obvious difference in the cytoplasm between competent and non-competent cells. The data indicate that the competence of E. coli cells might correlate with Ca accumulation in the periplasm rather than that in the cytoplasm of E. coli cells.
Chinese cabbage is one of the most popular and worldwide-consumed vegetables with abundant resource and has been intensively investigated for microspore culture. Microspore is mononuclear cell released from tetraploid...
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Chinese cabbage is one of the most popular and worldwide-consumed vegetables with abundant resource and has been intensively investigated for microspore culture. Microspore is mononuclear cell released from tetraploid after male gametophyte meitotic division, which can improve dormant gene's selection frequency and facilitate excellent character screening after doubling haploid. RAPD (Random Application Polymorphism DNA) has been popularized for its simplicity, rapidity and non-specialty among different genera and was extensively applied in genetic diversity and germplasm classification without knowing genetic background.
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