Objective The objective is to analyze tissue specific expression of BnMAPKlgenes, and explore their characteristics and signaling pathway in wound and Pathogenetic fungi stress Method On the basis of silico cloning,th...
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Objective The objective is to analyze tissue specific expression of BnMAPKlgenes, and explore their characteristics and signaling pathway in wound and Pathogenetic fungi stress Method On the basis of silico cloning,the promoters of BnMAPK1 were *** specificities in various organs of BnMAPK1 and mRNA expression characteristics after induced were analyzed by Quantitative real-time PCR（qPCR）.Result The cloned promoter sequences of BnMAPK1 contain several hormone and stress responsive ***1 expression could be induced by phytohormones methy1 jasmonate（MeJA）,salicylic acid（SA）,abscisic acid（ABA）, hydrogen peroxide（HO）,wound,and sclerotinia *** experiments showed that PDF1.2 gene was activated but PR-1 was suppressed after wound and *** treatments, indicate that oilseed rape most likely through jasmonic acid（JA） signaling in response to wound and pathogenetic fungi *** BnMAPK1 expression could be induced by several phytohormones and it's promoter contain several hormone and stress responsive elements BnMAPK1 may play an important role in resistance to a variety of stress in plants and oilseed rape might be mediated by JA signaling pathway in response to wound and pathogenetic fungi stress.
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