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Differential antioxidant responses to cold stress in cell suspension cultures of two subspecies of rice

Differential antioxidant responses to cold stress in cell su...

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中国遗传学会第九次全国会员代表大会暨学术研讨会

作者： Xin Wang Gen Fang Yang Li Meng Ding Hanyu Gong Yangsheng Li State Key Laboratory of Hybrid Rice College of Life SciencesWuhan University

Crops often encounter oxidative stresses due to changes of environmental conditions Cold damage is one of the serious problems to agricultural *** this study,cell suspension cultures of two subspecies of rice(Oryza sativa L.),Japonica *** and indica cv.9311, were developed to investigate their antioxidant responses to cold *** inherent morphology and surface features of cultured cells were examined by use of Cryo-scanning electron microscopy *** transcript of OsDREB2B,a cold-inducible transcription factor encoding gene,was strongly induced by cold stress in both cell lines.A significant accumulation of malondialdehyde,a product of lipid peroxidation,in cv.9311 cells exposed to low temperature after 24 h demonstrated that they had higher levels of membrane damage compared with *** under cold stress In addition,responses of the antioxidant system to cold stress were genotype *** to cv.9311,cell suspension cultures of *** exhibited elevated levels/contents of glutathione peroxidase,higher catalase,glutathione reductase,and *** would serve to protect cells against oxidative injury during the first few hours of chilling stress.

关键词： Oryza sativa L. OsDREB2B Antioxidant response Low temperature

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Transgenic rice expressing ictB and FBP/SBPase derived from Cyanobacteria exhibits enhanced photosynthesis and mesophyll conductance to CO2

Transgenic rice expressing ictB and FBP/SBPase derived from ...

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中国遗传学会第九次全国会员代表大会暨学术研讨会

作者： Han Y.Gong Yang Li Sun Sa Dao H.Hu Yang S.Li State Key Laboratory of Hybrid Rice College of Life SciencesWuhan University Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China College of Life SciencesSouth-Central University For Nationalities

In order to find a way to promote the rate of carbon flux and further improve photosynthetic rate in rice（Oryza sativa L.）.Two CO-transporting and fixing relevant genes ictB and FBP/SBPase derived from cyanobacterium arranged with respective 35SMV promotor as a whole construct were introduced into Oryza sativa *** *** homologous transgenic lines in T generation were selected according to the molecular biological experimental assays,and the functional effect of these two genes acting on improving the rate of carbon flow and chloroplastic CO concentration,further enhancing photosynthetic rate in rice mesophyll cells were investigated by means of the physiological biochemical experiments and analysis of leaf anatomy Our results indicated that photosynthetic rate,chloroplastic CO concentration（C） and mesophyll conductance（gm） in transgenic lines were higher about 11.5-21.7%,24.2-37.6%and 21.6-27.7% but without any changes in leaf anatomy structure compared to wild types *** multi-gene transformation technology,functional synergistic interaction of the two genes for improving CO2-transporting and assimilation rate and potential utility value for improving yield in rice were discussed.

关键词： rice Cyanobacteria photosynthesis Mesophyll Conductance to CO2

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The ComP-ComA quorum system is essential for "Trojan horse" like pathogenesis in Bacillus nematocida

The ComP-ComA quorum system is essential for "Trojan horse" ...

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中国遗传学会第九次全国会员代表大会暨学术研讨会

作者： Xidan Deng Yunxia Tian Xiao'e Xu Hanbo Zhang Qiuhong Niu Lianming Liang Ke-Qin Zhang Xiaowei Huang Laboratory for Conservation and Utilization of Bio-Resources and Key Laboratory for Microbial Resources of the Ministry of EducationYunnan University Tianjin Institute of Industrial Biotechnology.Chinese Academy of Sciences School of life science and technology Nanyang Normal University

Bacillus nematocida B16 has been shown to use "Trojan horse" mechanism in pathogenesis that has characteristics of "social" *** ComP-ComA system,a conserved quorum sensing system in the genus Bacillus,functions in many physiological processes including competence development,lipopeptide antibiotic surfactin production,degradative enzyme production and even some unknown *** we investigated the requirement of ComP-ComA system in *** B16 for its pathogenicity against *** comP mutant displayed deficiencies in attracting and killing nematodes,due to the absence of attractive signal molecules and the decreased expressions of virulence factors,*** data from transcriptional analysis further confirmed that this signaling system directly or indirectly regulated the expressions of two major virulence proteases in the infection of *** B16 Bioinformatics analyses from comparative genomics also suggested that the potential target genes of transcription factor ComA were involved in the processes such as the synthesis of attractants, production of extracellular degradative enzymes and sortase,secondary metabolites biosynthesis, regulation of transcription factors,mobility,as well as transporters,most of which were different from a saprophytic relative *** *** investigation firstly revealed that the participation and necessity of ComP-ComA signaling system in bacterial pathogenesis.

关键词： Bacillus The ComP-ComA quorum system is essential for Trojan horse like pathogenesis in Bacillus nematocida

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全基因组外显子测序首次发现CMD的常染色体隐性致病基因GJA1突变

全基因组外显子测序首次发现CMD的常染色体隐性致病基因GJA1突变

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中国遗传学会第九次全国会员代表大会暨学术研讨会

作者：胡颖 Ernst Reichenberger Salome de Almeida Valdenize Tiziani Kalpana Gowrishankar 首都医科大学附属北京口腔医院 University of Connecticut Health Center

颅骨干垢端发育不良(Craniometaphyseal dysplasia,CMD)是一种罕见骨骼硬化性疾病,主要表现为颅面骨进行性硬化。常染色体显性遗传的CMD已经被证实是由于焦磷酸盐转运体ANKH基因突变所导致。尽管CMD常染色体隐性模式(AR CMD)的致病基因... 详细信息

颅骨干垢端发育不良(Craniometaphyseal dysplasia,CMD)是一种罕见骨骼硬化性疾病,主要表现为颅面骨进行性硬化。常染色体显性遗传的CMD已经被证实是由于焦磷酸盐转运体ANKH基因突变所导致。尽管CMD常染色体隐性模式(AR CMD)的致病基因早已通过连锁分析定位到6q21.22染色体区域,但是基因突变一直未见报道。我们对一个葡萄牙AR CMD患者进行全基因组外显子测序,发现GJA,(gap junction protein alpha-1)基因C-末端的一个纯合错义突变(c.716G>A,***239Gln)在家系中共分离,并且该突变随后分别在巴西和印度家系中得到验证。

关键词：全基因组外显子测序常染色体隐性 CMD 首次发现

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椰枣全基因组测序与分析

椰枣全基因组测序与分析

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中国遗传学会第九次全国会员代表大会暨学术研讨会

作者： Al-Mssallem Ibrahim S. 胡松年张晓伟谭军林强刘万飞于晓光刘久成潘林林张同武尹玉新辛成齐吴浩张广宇 Ba Abdullah MohammedM. 黄大维方永军 AlnakhliYasser O. 贾善刚殷安 Al-OwayyedSaad A. AlsaihatiBurair A. 赵多军张笋 Al-OtaibiNoha A. 孙高远 MajrashiMajed A. 李福森塔拉 AlhuzimiEman M. 王吉祥云权正 AlnassarNafla A. 王磊杨猛 Al-JelaifyRasha F. 刘凯高胜寒陈开富 AlkhaldiSamiyah R. 刘贵明张猛郭海燕于军中沙联合卓越研究中心(中国科学院北京基因组研究所沙特阿拉伯阿卜杜拉国王科技城)

椰枣是中东和北非地区广泛种植的作物,具有重要的经济价值和历史文化意义。为了揭示椰枣的生物多样性、地理种群的分布、抗旱和抗逆的分子机理、性别决定(雌雄异株植物)与分子育种研究等,我们开展了椰枣基因组和转录组测序和分析工作。

关键词：全基因组测序

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Lesion mimic phenotype of spl5 was negatively regulated by a splicing factor 3b subunit 3(SF3b3) gene in rice

Lesion mimic phenotype of spl5 was negatively regulated by a...

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中国遗传学会第九次全国会员代表大会暨学术研讨会

作者： CHEN Xi-feng HAO Liang JIN Yang GU zhi-min MA Bo-jun College of Chemistry and Life Sciences Zhejiang Normal University

A lesion-mimic phenotype in rice(Oryza sativa L.) spotted leaf5(spl5) indicates that wild-type SPL5 negatively regulates cell death and resistance ***,the spl5 gene was already mapped to the 80-kb region between two markers SSR7 and RM7121 through a map-based cloning ***,we further showed that the spl5 gene was delimitated into a 15.l-kb genomic region by the high-resolution sequence target site(STS)*** sequencing in this region of spl5 mutant revealed that one candidate gene harbored a single-base deletion,resulting in a frame-shift mutation and a premature stop *** analysis showed that SPL5 gene encodes a putative splicing factor 3b subunit 3(SF3b3) and might be involved in splicing reactions of pre-mature RNAs participating in the regulation of cell death and resistance *** analysis showed that wild-type SPL5 did functionally complement the spl5 *** data presented here clearly indicate that the SPL5 negatively regulates cell death and resistance responses via modulating RNA splicing in plants

关键词： rice lesion mimic mutant spl5 map-based cloning SF3b3

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Research Advances of Hybrid Sterility in Rice

Research Advances of Hybrid Sterility in Rice

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中国遗传学会第九次全国会员代表大会暨学术研讨会

作者： LIU Yao-guang State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources College of Life SciencesSouth China Agricultural University

Hybrid sterility is a major form of postzygotic reproductive isolation between the species and subspecies in Oryza genus.A number of gene loci are involved in hybrid sterility in *** far,the cloning of several hybrid sterility genes have been reported,including S5(Chen et al.,2008,Yang et al.,2012),SaF/SaM(Long et al.,2008),S27/S28(Yamagata et al.,2010) and DPL1/DPL2(Mizuta et al.,2010).Three closely linked genes in S5 locus interact to control the female sterility of the indica-japonica hybrids of the Asia cultivated rice,while nucleotide variations in these genes produced three incompatible(S5-i,S5-j) and compatible(S5-n) alleles SaF and SaM form a complex locus Sa,and they encode a F-box/FBD protein and a SUMO-E3 ligase,*** have provided a "two gene/three component interaction model" to explain the selective killing of microspores carrying SaM-j allele in the indica-japonica *** we found that two other proteins also interact with this complex in controlling the pollen abortion S27/S28 and DPL1/DPL2 are essential genes for pollen *** the interspecific hybrids, recombination in meiosis between the pair of loci located in different chromosomes produces a portion of sterile pollen lacking both *** recently isolated several new genes controlling rice hybrid sterility with different molecular *** information demonstrates that diverse molecular mechanisms have been produced for reproductive barriers in rice speciation.

关键词： Rice Hybrid sterility Reproductive isolation Speciation gene

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Genetically controlled humanized microenvironment in mice for pre-clinical study

Genetically controlled humanized microenvironment in mice fo...

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中国遗传学会第九次全国会员代表大会暨学术研讨会

作者： CHEN Ye JACAMO Rodrigo KONOPLEVA Marina GUAN Min-xin ANDREEFF Michael Institute of Genetics College of Life SciencesZhejiang University Department of Leukemia The University of Texas M.D.Anderson Cancer Center

The importance of the microenvironment for cancer development,progression and resistance to treatment has recently been *** group was first to report the contribution of bone marrow（BM） derived mesenchymal stromal cells（MSC） for tumor development and *** provides dynamic microenvironment（niche） for the maintenance of hematopoietic stem cells（HSC） quiescence and normal hematopoiesis with high local concentrations of growth factors,chemokines and *** evidence has also shown that the BM microenvironment plays a pivotal role in the pathophysiology and propagation of leukemia Leukemia cells undergo spontaneous apoptosis once they are removed from the in vivo microenvironment and placed in suspension cultures without supportive *** understanding of the interactions between leukemia cells and their BM niche is critically important for leukemia *** here describe a novel artificial bone and bone marrow model mimicking the human hematopoietic microenvironment by using human mesenchymal stromat cells（MSCs） and endothelial colony-forming cells（ECFCs）.MSCs and ECFCs were isolated from heparinized human bone marrow or peripheral blood through an initial adhesion step,grown in specific media and then subcutaneously injected into the flanks of the NOD/SCID/IL-2rγ mice,where they developed into bone-like tissues with high osteoblast activity after 8 *** stains confirmed the bone structures and also showed that these artificial bones contained typical bone marrow cavities constituting a robust hematopoietic *** vivo imaging with Osteosense confirmed the presence of hydroxylapatite and luciferase imaging of firefly luciferase labeled human leukemic cells the engraftment of MOLM13/Luc/GFP leukemic cells in the extramedullary BM sites,following *** with infiltration of the extramedullary and physiological medullary bone marrow *** critical for MSC support of normal *** hematopoie

关键词： Mouse model Genetically controlled humanized microenvironment Tumor engraftment

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Compound Heterozygous MYO7A Mutations in a Chinese Pedigree with Nonsyndromic Hearing Loss

Compound Heterozygous MYO7A Mutations in a Chinese Pedigree ...

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中国遗传学会第九次全国会员代表大会暨学术研讨会

作者： CHEN Chao CHEN Dan-ni ZHENG Jing GONG Sha-sha ZHU Xu-fen Guan Min-xin Department of Genetics College of Life SciencesZhejiang University Attardi Institute of Mitochondrial Biomedicine and Zhejiang Provincial Key Laboratory of medical Genetics.School of Life Sciences Wenzhou Medical University Attardi Institute of Mitochondrial Biomedicine and Zhejiang Provincial Key Laboratory of medical Genetics School of Life SciencesWenzhou Medical University

MYO7A gene encodes an unconventional myosin,which is essential for well functioned hair cells of inner *** in MYO7A have been associated with nonsyndromic hearing loss Here we report clinical and genetic characterization of a Chinese pedigree carrying compound heterozygous ***7A is considered to be responsible for the WLT151 pedigree by exome capture sequencing and pathogenicity *** proband has less severe hearing loss in low frequency without defects in vestibular or retinal,though her parents have normal hearing After evaluation by PCR and sequencing,the proband is confirmed to carry a missense mutation c.l184G>A(***395His) and a nonsense mutation c.4117C>T(***1373Ter),the former of which is inherited from her father's de novo mutation,and the latter of which is from her mother. These compound heterozygous mutations are in functional domains probably causing damaged *** analysis shows the two sites are highly conserved in 19 vertebrates,with absent allelic frequency in the ***,these data strongly indicate the WLT151 pedigree has autosomal recessive nonsyndromic deafness(DFNB2).

关键词： MYO7A gene nonsyndromic hearing loss compound heterozygous mutation

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Establishment and gene expression characteristics of the silk gland cell line BmSG-SWU1 of the silkworm,Bombyx mori

Establishment and gene expression characteristics of the sil...

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中国遗传学会第九次全国会员代表大会暨学术研讨会

作者： Zhen-Yue Feng 潘春 Min Liu Zhi-Qiang Tian Qian He Tai-Hang Liu Cheng Lu 潘敏慧 State Key Laboratory of Silkworm Genome Biology Southwest University Institute of Life Science Technology Heilongjiang Bayi Agriculture University

A BmSG-SWU1 cell line was established from the silk gland tissues of newly hatched Bombyx mori larvae by performing primary cultures for two *** cell line was comprised of long and thin shuttle *** percentage of cells with the chromosome number 2n=56 was 76.28%.Therefore,the cell line was considered a diploid cell *** analysis of BmSG-SWU1 and four other cell lines indicated that BmSG-SWU1 had a specific fingerprint,and that the genetic relationship between BmSG-SWU1 and the original silk gland tissue was the *** employed whole genome microarray data,RT-PCR and FQ-PCR to analyze the expression profile of *** day three of the fifth instar silk gland,the two known silk gland-specific genes Fib-L and P25 as well as 18 other highly expressed genes were found in the whole genome microarray *** some of these were expressed in the cell line BmSG-SWUI, which showed that this cell line had maintained some gene expression characteristics of a silk *** key cell cycle-control genes BmCyclinB and BmCyclinB3 of the G2 and M phase were expressed at low levels in silk ***,they were highly expressed in BmSG-SWUI,in the ovaries and other cell lines,which suggested that BmCyclinB and BmCyclinB3 are important for the transformation of BmSG-SWU1 from endoreduplication to the mitotic cycle.

关键词： Bombyx mori silk gland cell line gene expression

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