Recent studies have shown that F-box proteins constitute a large family in eukaryotes and play pivotal roles in regulating various developmental processes in ***,their functions in monocots are still *** this study,we...
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Recent studies have shown that F-box proteins constitute a large family in eukaryotes and play pivotal roles in regulating various developmental processes in ***,their functions in monocots are still *** this study,we characterized a recessive mutant dwarf and deformedflowerl-1(ddf1-1) in *** mutant is abnormal in both vegetative and reproductive development,with significant size reduction in all organs except ***1 controls organ size by regulating both cell division and cell *** the ddf1-1 spikelet,the specification of floral organs in whorls 2 and 3 is altered,with most lodicules and stamens being transformed into glume-like organs and pistil-like organs,respectively;but the specification of lemma/palea and pistil in whorls 1 and 4 is not ***1 encodes an F-box protein anchored in nucleolus and is expressed in almost all vegetative and reproductive *** with the mutant floral phenotype,DDF1 positively regulates B-class genes OsMADS4/16,and negatively regulates pistil specification gene *** addition,DDF1 also negatively regulates the Arabidopsis LFY ortholog APO2,implicating a functional connection between DDF1 and ***,these results revealed that DDF1,as a newly identified F-box gene,is a crucial genetic factor with pleiotropic functions for both vegetative growth and floral organ specification in *** findings provide additional insights into the molecular mechanism controlling monocot vegetative and reproductive development.
叶绿体ATP合成酶的主要功能是利用光合电子传递建立的跨膜质子梯度来合成ATP,它是一个由20多个亚基以一定比例(Ⅰ1Ⅱ1Ⅲ14Ⅳ1和α3β3γ1ε1δ1)组成的大分子蛋白复合物,包括镶嵌在膜内起质子通道作用的CF0和突出于膜外起催化作用的CF1两部分。叶绿体ATP合成酶的生物发生不但需要核基因组与叶绿体基因组的相互协作,还需要一些特殊的核基因编码的调节因子的精密调控。为研究叶绿体ATP合成酶的生物发生机理,我们建立了一套特异性筛选叶绿体ATP合成酶突变体的筛选体系。通过对拟南芥突变体库进行大规模筛选,获得了多个ATP合成酶累积量降低的突变体。进而利用图位克隆、Tail-PCR等方法克隆到两个突变体的突变基因,其分别编码一个功能未知的叶绿体基质蛋白(FBAl和FBA3,Factorsrequired for Biogenesis of the chloroplast ATP synthase)。酵母舣杂交实验表叫FBA1和FBA3分别与ATP合成酶的γ,和β相互作用,表明这两个生物发生因子可能通过与特定的ATP合成酶亚基相互作用而调控ATP合成酶的生物发生。
作者：
刘旭东高凌寒张蕊赵阿曼纪宝虎贺林马捷Department of Orthopaedic Surgery
Shanghai Sixth People's Hospital Affiliated to Shanghai Jiaotong University Bio-X Institutes
Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders(Ministry of Education)Shanghai Jiao Tong University Department of Genetics and Molecular Biology
Xi'an Jiaotong University School of Medicine Bio-X Institutes
Key Laboratory for the Genetics of Developmental and Ncuropsychiatric Disorders(Ministry of Education)Shanghai Jiao Tong University Department of Psychiatry
University of California Bio-X Institutes
Key Laboratory for the Genetics of Developmental and NeuropsychiatricDisorders(Ministry of Education)Shanghai Jiao Tong University
Brachydactyly type A2（BDA2,MIM 112600） is characterized by the deviation and shortening of the middle phalange of the index finger and the second *** carried out a genetic analysis using a Chinese BDA2 family to giv...
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Brachydactyly type A2（BDA2,MIM 112600） is characterized by the deviation and shortening of the middle phalange of the index finger and the second *** carried out a genetic analysis using a Chinese BDA2 family to give genetic *** the pedigree,we mapped the the maximum candidate interval of BDA2 to a～1.5Mb region between D20S194 and D20S115 within chromosome 20p12.3,and found that the pairwise logarithm of odds score was highest for marker D20S156(Z=6.09 atθ=0).Based on functional and positional perspectives,Bone morphogenetic protein 2（BMP2） gene was identified as the causal gene for BDA2 in this region though no point mutation was detected in *** the further investigation,we identified a 4.6 kb genomic duplication at the downstream of BMP2 *** duplication is located within the linked region,co-segregated with the BDA2 phenotype in this family but not in the unaffected family members and the unrelated control *** summary,we detected a duplication of 4.6 kb in BMP2 locus in a Chinese family affected with *** finding supports that the genomic location that corresponds to the duplication region is a mutational hotspot and that this genomic location is critical for the function of BMP2.
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