INTRODUCTION Gene transfer vector is one of the key technologies of gene *** carriers generally can be divided into two major categories of virus and non-virus,the former is subjected to security *** latter much favor...
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INTRODUCTION Gene transfer vector is one of the key technologies of gene *** carriers generally can be divided into two major categories of virus and non-virus,the former is subjected to security *** latter much favored in medical science and also is currently the main object of pharmaceutical research because of its safety,vector design diversity and low *** non-virus carrier,polycation gene carrier due to the high rate of transfection and become a main research ***(PAMAM) is one of the important member in the family of cationic polymer carrier,PAMAM dentrimers are attracted highly attention recently due to their special properties in the drug and gene delivery systems,especially the high generation PAMAM as the gene vector shows strength transfection ability compared with the other non-viral carrier *** main drawback for the materials is presenting serious cell *** order to overcome this problem,one new gene delivery system has been designed by our research *** AND DISCUSION This is that PAMAMs are covalently conjugated onto a-cyclodextrin(a-CD) via amide bonds to obtain the starburst-like cationic polymers(CD-PG2...he chemical structure and composition of CD-PG2...s characterized by *** physicochemical and biological properties of CD-PG2...NA polyplex were evaluated by agarose gel retardation,stability test against DNase I,MTT assay,DLS measurement,CLSM observation,LDH leakage test,and in vitro cell *** test results tell us that the CD-PG2...uld efficiently condense pDNA into nano-scale particles with a narrow size distribution,and protest from DNase Ⅰ *** CD-PG2...ows excellent gene transfection efficiency without serum interference as well as relatively low cytotoxicity,compared with free PET-2... and commercial product Lipofectamine 2...(one good gene vector),.The cell uptake route analysis shows cellular uptake CD-PG2...NA polyplex is mainly through Cl
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