Present by You -Lin Qiao, M. D.;Ph. D. , Professor and chief, Dept. of Epidemiology, CICAMS BACKGROUND: Esophageal cancer is the fourth leading cause of cancer death in China. Linxian has one of the highest mortality ...
详细信息
Present by You -Lin Qiao, M. D.;Ph. D. , Professor and chief, Dept. of Epidemiology, CICAMS BACKGROUND: Esophageal cancer is the fourth leading cause of cancer death in China. Linxian has one of the highest mortality rates of esophageal cancer in the world. Many previous studies from Linxian , have shown that esophageal squamous dysplasia (ESD) is an important precursor lesion of esophageal squamous cancer (ESC) in this high risk area, and that the histology grading of dysplasia correlates with the risk of developing invasive cancer. Thus, the grade of ESD appears to be an appropriate interme-
目的研究FasL、B7-1基因修饰的肿瘤细胞诱导抗食管癌CTL的活化,探讨FasL、B7-1联合基因转移是否具有协同的抗肿瘤效应。方法采用重组腺病毒载体将FasL、B7-1基因导入人食管癌细胞Eca-109中,G418阳性克隆筛选,流式细胞分析、RT-PCR、显示FasL和B7-1的表达,将携带FasL和B7-1基因的食管癌细胞(命名为Eca-109/FB-11)接种于C57BL/6小鼠背部皮下,其致瘤性显著下降(p<0.01);Eca-109/FB-11致敏的小鼠对野生型瘤细胞具有免疫保护作用(p<0.05);用Eca-109和Eca-109/FB-11细胞分别经腹腔免疫小鼠,得到腹腔浸润淋巴细胞及致敏脾细胞,MTT法进行体外杀伤实验。结果FasL和B7-1基因在食管癌细胞中获得高表达,Eca-109/FB-11诱导的CTL(cytotoxic T lymphocyte)对Eca-109的杀伤活性显著高于野生型Eca-109诱导的CTL对相同靶细胞的杀伤活性(p<0.05);而且Eca-109/FB-11诱导的CTL对Eca-109/FB-11的杀伤率显著高于对野生型Eca-109的杀伤率(p<0.05)。结论FasL促进靶细胞cis和trans凋亡,B7-1促进抗食管癌CTL的增殖、活化,尤其在效应阶段发挥着重要的协同作用。
暂无评论