The toxic effects or biomedical functions of nanoparticles largely depend on their translocation ***,because of a large number of variable parameters of nanoparticles,experimental measurements of each type of nanopart...
详细信息
The toxic effects or biomedical functions of nanoparticles largely depend on their translocation ***,because of a large number of variable parameters of nanoparticles,experimental measurements of each type of nanoparticles in vivo become a huge work and almost ***,establishing theoretical models for rapid analyses will be beneficial to both safety estimation and application of *** the present study,the size-dependent translocation mode and biological fate of intranasally instilled FeO nanoparticles（40 nm and 280 nm）in CNS were *** nanoparticle translocation in different parts of brain at 4h,12h,24h,3d,7d,and 30d were quantified using ICP-MS technique.A biexponential model（correlation coefficient r=0.98～0.99）was satisfactory to describe the particokinetic translocation behavior of FeO nanoparticles in *** found a size-dependent translocation pattern and a size-sensitive time-window（4-72 h）of the nanoparticles in the brain,which are most significant in toxic concerns of nanoparticles in the *** synchrotron-based techniques such as microbeam X ray fluorescence（SR-μXRF） and near-edge X-ray absorption spectroscopy（XANES）were then used to map the spatial microdistribution and to identify the chemical forms of the nanoparticles in brain,respectively. The SR-μXRF images showed the 40 nm-FeO particles more widely distributed in brain regions than the large FeO particle *** XANES results indicate that the presence of chemical speciation of the FeO nanoparticle（～15%）and protein-complex like apotransferrin-FeO（～16%）in brain,implying that self-coating of FeO nanoparticles occurred with transferrin in *** but not the least,all the findings suggest size-sensitive manners of nanoparticles in brain,the smaller one possesses evident detention properties in the CNS *** larger one.
An integrated metabonomics study using high-resolution H NMR spectroscopy has been applied to investigate the biochemical composition of urine,serum,liver tissue aqueous extracts（acetonitrile/water）and liver tissue ...
详细信息
An integrated metabonomics study using high-resolution H NMR spectroscopy has been applied to investigate the biochemical composition of urine,serum,liver tissue aqueous extracts（acetonitrile/water）and liver tissue lipidic extracts（chloroform/methanol） obtained from control and Bay41-4109 treated rats(10,50,400mg·kg·dfor 5 days,i.g.)or phenobarbital treated rats(60mg·kg·dfor 5 days,i.p.).Principal components analysis was used to visualize similarities and differences in biochemical *** results showed the biochemical profiles of 400mg·kg·dgroup might reflect the hepatotoxicity of Bay41-4109 more *** elevation in the level of 3-HB,lactate,2-hydroxy-acetol,hydroxymaleic acid and d-glucose was found in the urine,and the levels of VLDL/LDL（CH）n, VLDL/LDL-CH,2-oxo-3-methyl-n-valerate,3-HB,lactate,pyruvate,taurine, 3-amino-isovalerate,2-hydroxy-isovalerate in serum were increased significantly in 400mg·kg·*** predominant changes identified in liver tissue aqueous extracts included an increase in the signal intensities of lactate,3-amino-isovalerate,pyruvate,taurine, choline,TMAO and a reduction in the intensities of trytophan and *** liver tissue chloroform/methanol extracts,there was a remarkably increase in many of the lipid signals including the triglyceride terminal methyl,methylene groups,and CHCO,N（CH）, CHOPO,*** observations all provide evidence that fatty acid metabolism disorder and mitochondrial inability might contribute to the hepatotoxicity of Bay41-4109. There were similarities and differences between the mechanisms of the hepatotoxicity of Bay41-4109 and *** application of H NMR spectroscopy to an array of biological samples comprising urine,serum and liver tissue extracts yields new insight into the hepatotoxicity of xenobiotics.
暂无评论