Objective: Patients with minor ischemic stroke(MIS) are at a high risk of recurrent ischemic stroke(RIS).However,few studies to investigated the effects of gene variants on antiplatelet drug responsiveness in *** aim ...
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Objective: Patients with minor ischemic stroke(MIS) are at a high risk of recurrent ischemic stroke(RIS).However,few studies to investigated the effects of gene variants on antiplatelet drug responsiveness in *** aim of this study was to investigate the association of platelet receptor gene(P2Y12, P2Y1) and glycoprotein gene(GPIIIa) variants, as well as their interactions with antiplatelet drugs responsiveness and clinical outcomes in acute MIS. Methods: We prospective consecutively enrolled 426 patients with acute MIS who were receiving combined treatment of aspirin and clopidogrel for at least 3 *** seven variants from P2Y12, P2Y1 and GPIIIa genes were examined using mass spectrometry. Gene-gene interactions were analyzed using generalized multifactor dimensionality reduction(GMDR) analysis. Antiplatelet drugs responsiveness was assessed by platelet aggregation *** patients were followed up for 90 days. Primary outcomes were evaluated as a composite of RIS, myocardial infarction(MI) and death. Results: Among the 426 patients, the primary adverse events occurred in 46(10.8%) patients(40 had RIS, 2 died, and 6 had MI) during the first 90 days after stroke. There were no significant differences in the frequencies of the genotypes of the seven variants between the patients with and without adverse events using single-locus analytical approach. However, there was significant gene-gene interaction between rs16863323 and rs2317676. The high-risk interactive genotypes were independently associated with antiplatelet drug responsiveness and clinical outcomes. Conclusion: Sensitivity of MIS patients to antiplatelet drugs and adverse clinical events maybe multifactorial, but is not determined by single gene polymorphisms.
Objective: The association of genetic variants with aspirin resistance(AR) remains controversial. The aim of this study was to assess the correlation of relevant genetic variants with AR and early clinical outcomes in...
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Objective: The association of genetic variants with aspirin resistance(AR) remains controversial. The aim of this study was to assess the correlation of relevant genetic variants with AR and early clinical outcomes in Chinese patients with acute ischemic stroke(IS). Methods: Eight hundred and fifty acute IS patients were consecutively enrolled. Platelet aggregation activity was measured before and after a 7-10 day aspirin treatment. The DNA sequences of 14 variants of COX-1, COX-2, GPIb, GPIIIa, P2Y1 and P2Y12 genes were examined using a matrix-assisted laser desorption/ionization time of flight mass spectrometry. Gene-gene interactions were analyzed using generalized multifactor dimensionality reduction(GMDR). The primary outcome was early neurological deterioration(END) within the first 10 days after *** secondary outcome was a composite of early recurrent ischemic stroke(ERIS), myocardial infarction(MI), and death during the first 10 days after admission. Results: Among the 850 acute IS patients, 175(20.6%) had AR, 45(5.3%) had aspirin semi-resistance(ASR), 121(14.2%) developed END, 17(0.2%) had ERIS, 2(0.2%) died, and 6(0.7%) had MI. Apart from rs1371097 variant, there were no associations between other variants and AR using the single locus analysis. However, GMDR analysis demonstrated there was significant gene-gene interaction among rs20417, rs1371097, and rs2317676 with rs20417 CC, rs1371097 TT, and rs2317676GG;rs20417 CC, rs1371097 TT, and rs2317676GG/AG;rs20417 CC, rs1371097 CT, and rs2317676 AG the high-risk interactions for AR in IS patients. The frequency of END was significantly higher in patients with AR and carrying high-riskinteractive genotypes. Moreover, AR and high-risk interactive genotypes were independently associated with END. Conclusion: Sensitivity of IS patients to aspirin and END may be multifactorial and is not determined by single gene polymorphisms. The combinational analysis used in this study may provide further insight int
Objective: Accumulating studies have indicated that immune dysfunction might be involved in the physiopathology of schizophrenia and aggression. This study aims to investigate the correlation between high sensitive C-...
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Objective: Accumulating studies have indicated that immune dysfunction might be involved in the physiopathology of schizophrenia and aggression. This study aims to investigate the correlation between high sensitive C-reactive protein(hs CRP), interleukin(IL)-10 and clinical characters especially aggression, and also explore the potential role of hs CRP and IL-10 as plasma biomarkers of schizophrenia. Methods: Forty-one patients of schizophrenia and forty age and gender matched healthy individuals were enrolled. Psychopathological severity and aggression were assessed using Positive and Negative Symptom Scale(PANSS) and Modified Overt Aggression Scale(MOAS). The blood sampling and assessments were performed at the time of enrolment. Plasma concentrations of hs CRP and IL-10 were assessed by Enzyme-Linked Immunosorbent Assay(ELISA) according to the manufacturer protocol. Results:(1) Higher levels of hs CRP(p<0.001), lower levels of log IL-10(p<0.001)and higher ratios of hs CRP to IL-10(p<0.001) were observed in plasma of patients with schizophrenia compared to healthy controls;(2) ROC(Receiver Operating Characteristic) curve analysis revealed that ratio of hs CRP/IL-10(Predictive value: 0.783, p<0.01;Sensitivity: 85.4%;Specificity: 67.5%) was more applicable as a biomarker to distinguish schizophrenia from control group than hs CRP and IL-10 alone(Predictive value: 0.718, p<0.01;0.275, p<0.001, respectively);(2) There were significant negative correlations between hs CRP and score of thought disorder of PANSS(r=-0.355,p<0.05), between log IL-10 and the score of general symptoms and total score of PANSS(r=-0.397, p<0.05;r=-0.325, p<0.05), and positive correlation between hs CRP/IL-10 and the score of general symptoms of PANSS(r=0.393, p<0.05);(3) We also found positive correlations between hs CRP and the total score and verbal aggression score of MOAS(r=0.654, p<0.01;r=0.678, p<0.05), and between hs CRP/IL-10 and the total score of MOAS(r=0.636, p<0.01). Conclusions: O
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