Tomato（Lycopersicon esculentum cv.'Moneymaker'） was transformed with a codA gene,from Arthrobacter globiformis,for choline oxidase that had been modified to allow targeting to both chloroplasts and the ***...
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Tomato（Lycopersicon esculentum cv.'Moneymaker'） was transformed with a codA gene,from Arthrobacter globiformis,for choline oxidase that had been modified to allow targeting to both chloroplasts and the ***（GB） accumulated in seeds of transformed plants up to 1μmol g DW,while no detectable GB was found in wild-type *** incubation at temperatures from 25℃to 55℃for 90 min during imbibition,codA-transgenic seeds germinated faster and at higher frequency than wild-type *** the exposure of imbibed seeds to 40°/30℃（12 h/12 h） or 34°/34℃（16 h/8 h）, transgenic seeds germinated faster and developed more vigorous seedlings than wild-type *** compared the expression of genes for a mitochondrial small heat-shock protein（MT-sHSP）,heat-shock protein 70（HSP70）,and heat shock cognate 70（HSC70） between wild-type and transgenic seeds after heat stress at 34℃.Levels of expression of all three genes were higher in transgenic seeds than in wild-type seeds during heat stress,and the accumulation of HSP70 was more prominent in codA-transgenic seeds than in wild-type *** of GB at 0.1 mM to 5 mM to the germination medium or imbibition of seeds in a solution of GB enhanced the tolerance of wild-type seeds to high ***-type seeds that had been soaked in 1 mM GB for 20 h before high-temperature treatment also expressed heat-shock genes at elevated levels and accumulated more HSP70 than *** results suggest that GB,either applied exogenously or accumulated in vivo in codA-transgenic seeds,enhanced the expression of heat-shock genes in and improved the tolerance to high temperature of tomato seeds during germination.
MicroRNAs(miRNAs) are important for the regulation of gene expression,and are involved in many developmental processes.A set of miRNAs which were differentially expressed between cells of totipotent (C1) and non-totip...
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MicroRNAs(miRNAs) are important for the regulation of gene expression,and are involved in many developmental processes.A set of miRNAs which were differentially expressed between cells of totipotent (C1) and non-totipotent(C2) Arabidopsis thaliana calli was identified,some of which were affected during callus formation or shoot *** of those down-regulated after 10 days' incubation in shoot induction medium(SIM) was MIR160a,for which transcript abundance was lower in C1 than in C2. Over-expression of MIR160 compromised shoot regeneration from in vitro cultured *** cells,while the transgenic expression of a miR160-resistant form of ARF10 was associated with a high level of shoot *** latter transgenic line also showed an elevated expression level of shoot meristem-specific genes CLAVATA3,CUP-SHAPEDCOTYLEDON1 and -2,and WUSCHEL ARFW expression was concentrated at the initiation sites of shoots or leaves,while during the early phase of shoot regeneration,the accumulation of the ARFW mRNA was lower in the wild type than in the mARF10 transgenics,in contrast to the pattern of miR160 ***,miR160 and ARF10 both appear to be components of the regulation of shoot regeneration in vitro.
In a field experiment,two winter wheat(Triticum aestivum L.) cultivars,Jimai 22 and Xinmai 26,differing in their sensitivity to heat stress,were subjected to high temperature by establishing greenhouse at grain fillin...
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In a field experiment,two winter wheat(Triticum aestivum L.) cultivars,Jimai 22 and Xinmai 26,differing in their sensitivity to heat stress,were subjected to high temperature by establishing greenhouse at grain filling,to study the photosynthesis capability with various aspects under heat stress. Experimental results showed that the net photosynthesis rate declined under the heat stress,not caused by stomata limitation but by decline in Rubisco activity and functional drop of PSⅡ.Photosynthetic rates of flag leaf decreased faster in Xinmai 26 than in Jimai 22 under heat stress during grain filling,with a decrease of 12.8%and 6.6%,*** net photosynthesis rate reduced more rapidly than that under non-stress,and the reduction reached up to 69.9%and 59.3%at 10 *** slower photosynthetic decline contribute directly some to its higher grain yield in Jimai 22,together with its slower decrease of chlorophyll,flag leaf senescence was delayed in comparison to the earlier senescent Xinmai *** grain yield of Jimai 22 decreased by 6.41%,and that of Xinmai 26 decreased by 11.43%,compared with those *** stress also caused alteration to ultrastructure of mesophyll *** stress damaged the regular ultrastructure of many organelles in different extent,such as chloroplast,mitochondria,*** caused by heat stress on organelles in Xinmai 26 was more severe than those in Jimai ***,Jimai 22 cultivar showed some self-repair capacity to the impairment,yet Xinmai 26 cultivar did *** results indicated that photosynthetic performance decline caused by heat stress was cultivar-dependent. Jimai 22 cultivar had better heat stability in PSⅡfunction and in carboxylation activity,comparison to Xinmai 26 cultivar,which was susceptible to heat stress.
In this study,12 winter wheat cultivars,growing in phytotron,were used as *** seedlings of winter wheat were treated by ultraviolet-B radiation（UV-B,280nrn～320nm） for 15 d using the dose of 3.24 kJ·m·d an...
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In this study,12 winter wheat cultivars,growing in phytotron,were used as *** seedlings of winter wheat were treated by ultraviolet-B radiation（UV-B,280nrn～320nm） for 15 d using the dose of 3.24 kJ·m·d and 5.40 kJ·m·d,*** response indexes（RI） of plant height,dry weight, fresh weight,photochemical reflectance index（PRI）,chlorophyll,carotenoid and anthocyanin content were analyzed and *** results showed that the influences of the two UV-B doses on winter wheat seedlings were ***-B dose of 3.24 kJ·m·d significantly inhibited the height of plants, but promoted the dry weight and ***-B dose of 5.40 kJ·m·d significantly inhibited all the indexes, especially plant height and fresh *** the treatment of two UV-B doses,the RI of plant height, dry weight,fresh weight,carotenoid and anthocyanin content were all very significantly correlated with the cumulative stress response index（CSRI）,and the RI of carotenoid and anthocyanin content were significantly correlated with dry weight and fresh weight,indicating that carotenoid and anthocyanin content were vital to UV-B ***,there was no significant correlation in the RI of anthocyanin content between two doses of UV-B and the same result existed in the RI of carotenoid content,indicating that the responses of these two indexes were not consistent under different doses of *** shows a very significant correlation to carotenoid and anthocyanin content under UV-B of 3.24 kJ·m·d,indicating that the decline of carotenoid and anthocyanin content might be one of the reasons for PRI *** all samples,the modification of fresh weight under two doses of UV-B was variable and not significant,but the correlation of dry weight was significant,which might be the results of different effects on water content influenced by different doses of ***,the index of fresh weight was not suitable for analysis of UV-B *** conclusion,when comparing the UV-B toleran
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