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中国松树枯梢病菌(Sphaeropsis sapinea)遗传多态性的RAPD分析

中国松树枯梢病菌(Sphaeropsis sapinea)遗传多态性的RAPD分析

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江苏省植物病理学会第九届会员代表大会暨学术研讨会

作者：吴小芹黄敏仁尹佟明南京林业大学南京林业大学南京林业大学

松树枯梢病[Sphaeropsis sapinea(Fr.:Fr.)Dyko & Sutton]是世界范围内针叶树上的重要病害之一。该病在我国分布地域广,受害寄主多,发生危害严重。根据对该病菌营养体亲和性等方面的研究,发现其种群存在一定的分化现象。从遗传学观... 详细信息

松树枯梢病[Sphaeropsis sapinea(Fr.:Fr.)Dyko & Sutton]是世界范围内针叶树上的重要病害之一。该病在我国分布地域广,受害寄主多,发生危害严重。根据对该病菌营养体亲和性等方面的研究,发现其种群存在一定的分化现象。从遗传学观点来看,遗传多样性也意味着基因多样性,它决定了物种进化的潜势。一个物种的遗传变异越丰富,对环境变化的适应性也就越大。掌握病原真菌遗传变异的细微结构是进行群体遗传学和流行学研究的一个重要方面。因此,为了从根本上弄清***的种内变异情况,了解该菌对寄主与环境变化适性的潜能,本研究运用RAPD技术对从我国13个省(区)收集的该菌菌株进行种一级水平基因多态性的检测,以其从分子水平上揭示该病菌的遗传变异本质及其亲缘关系,为进一步弄明该菌的流行特点和实施综合治理提供参考依据。

关键词：地理来源松树枯梢病菌 Sphaeropsis sapinea 引物筛选多态性

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社会团体提升生态文明建设科普教育活动成效的策略研究

社会团体提升生态文明建设科普教育活动成效的策略研究

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中国生态经济学学会第九届会员代表大会暨生态经济与生态城市学术研讨会

作者：蔡卓平段舜山广东省生态学会广东省生态学会科普工作委员会广东省生态学会海洋生态专业委员会广东省生态学会生态健康人居与生态工程专业委员会

社会团体应该集中专家力量,结合学术特色,发挥专业优势,积极参与生态文明建设科普教育工作。本文介绍生态文明建设及其科普教育情况,分析社会团体开展生态文明建设科普教育工作的意义及其推动因素,总结社会团体开展生态文明建设科普教... 详细信息

社会团体应该集中专家力量,结合学术特色,发挥专业优势,积极参与生态文明建设科普教育工作。本文介绍生态文明建设及其科普教育情况,分析社会团体开展生态文明建设科普教育工作的意义及其推动因素,总结社会团体开展生态文明建设科普教育工作的实践模式,归纳社会团体开展科普教育过程中面临的困境,提出社会团体开展生态文明建设科普教育工作的提升策略。实例分析广东省生态学会近年在开展生态文明建设相关活动中的实践成果,以期为相关社会团体开展相关科普宣传工作提供有益参考。

关键词：生态文明科普教育社会团体策略

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AMHRⅡ及其基因甲基化在高雄激素PCOS大鼠模型中的研究

AMHRⅡ及其基因甲基化在高雄激素PCOS大鼠模型中的研究

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广东省遗传学会第九届代表大会暨学术研讨会

作者：韦相才郝栋栋钟兴明王瑾苗竹林王永霞广东省计划生育科学技术研究所

多囊卵巢综合征(PCOS)是以高雄激素、胰岛素抵抗为主的内分泌、代谢紊乱综合征。PCOS至今发病机制不明,近年来研究认为PCOS是一种卵源性疾病,卵巢局部调节因子的异常可能是导致PCOS一系列病例生理改变的重要因素。抗苗勒管激素(AMH)是... 详细信息

多囊卵巢综合征(PCOS)是以高雄激素、胰岛素抵抗为主的内分泌、代谢紊乱综合征。PCOS至今发病机制不明,近年来研究认为PCOS是一种卵源性疾病,卵巢局部调节因子的异常可能是导致PCOS一系列病例生理改变的重要因素。抗苗勒管激素(AMH)是有卵巢颗粒细胞分泌的一种局部调节因子,在PCOS患者血清中明显高于正常同龄妇女且研究认为其与卵泡生长发育密切相关。AMHRⅡ是AMH作用的中间受体,它的表达是否参与或者以何种方式参与AMH调控卵泡发育的过程,研究报道不一。DNA甲基化是基因表观遗传修饰的重要方式,AMHRⅡ基因在PCOS中是否存在着表观遗传学改变从而影响其在组织中的表达,至今鲜有报道。本实验采用免疫组织化学法检测高雄激素大鼠模型卵巢、子宫内膜等局部组织中AMHRⅡ的表达与分布;通过MSP定性分析模型组与对照组大鼠血液、卵巢组织DNAAMHRⅡ基因启动子区域的甲基化状态。免疫组织化学检测显示AMHRⅡ1定位表达于细胞膜,在卵巢间质及卵泡颗粒细胞中均存在阳性表达,且在模型组卵泡及间质中的表达强于对照组;AMHRⅡ在子宫内膜中也存在着表达,两组之间表达无明显差异。MSP结果显示模型组与对照组血液、卵巢组织所有DNA样本均检出部分甲基化条带,两者之间无差异。结果提示,AMHRⅡ可能参与了PCOS中AMH调控卵泡发育的过程,但可能与子宫内膜病变无明显相关关系。AMHRⅡ基因在启动子区域存在着部分甲基化,但这种甲基化状态可能与PCOS发病及局部组织病理变化无明显相关。

关键词：抗苗勒管激素Ⅱ型受体多囊卵巢综合征甲基化特异性PCR DNA甲基化大鼠模型

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A novel compound heterozygous mutation of ABCC8 gene causing a diazoxide-unresponsive congenital hyperins-ulinism with an atypical form:18F-DOPA-PET/CT scan of a focal lesion in the pancreas

A novel compound heterozygous mutation of ABCC8 gene causing...

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广东省遗传学会第九届代表大会暨学术研讨会

作者：张文刘丽 Jing Cheng Zhe Wen Cuiling Li Fenghua Wang Hongsheng Liu Department of genetics and endocrinology Guangzhou Women and Children's Medical Center

Congenital hyperinsulinism（CHI） is a severe heterogeneous disorder due to dysregulation of insulin secretion from the pancreatic P-cells leading to severe hypoglycemia in the neonatal,infancy and childhood *** we report a Chinese boy with CHI unresponsive to medical therapy with *** analysis identified a novel compound heterozygous mutation in ABCC8 *** heterozygous mutations in CHI result in complex *** novel mutation and phenotype correlation is *** with F-DOPA-PET/CT indicated a focal lesion in the head of the *** pathological diagnosis was an atypical form.F-DOPA-PET/CT is a useful tool in distinguishing between focal and diffuse disease preoperatively but the accuracy is not 100%.The scan result is best combined with genetic analysis and intra-operative *** report may provide new insights of genotype-phenotype correlations and management of CHI.

关键词： Congenital hyperinsulinism mutation ABCC8 18F-DOPA-PET/CT

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High resolution melting analysis for the neonatal screening of G6PD deficiency

High resolution melting analysis for the neonatal screening ...

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广东省遗传学会第九届代表大会暨学术研讨会

作者： Hui Yang Qian Wang Lei Zheng Xiao-Fen Zhan Min Lin Fen Lin Xin Tong Zhao-Yun Luo Yue Huang Li-Ye Yang Laboratory Medical Center Chaozhou Central Hospital Affiliated to Southern Medical University Laboratory Medical Center Nanfang HospitalSouthern Medical University

Introduction:Glucose-6-phosphate dehydrogenase(G6PD) deficiency is highly prevalent in southern *** aim of this study is to assess the extent of this disease in Chinese neonates and determine its molecular characteristics using a novel molecular screening ***:A total of 2500 neonates were routinely screened for G6 PD deficiency using a modified fluorescent spot test(FST).PCR-High Resolution Melting(HRM) Analysis was then used for the molecular ***:The overall incidence of G6 PD deficiency was 2.68%in our study *** in male population was 3.22%(44 neonates of 1365 male neonates) and in female population was2.03%(23 neonates of 1135 female neonates).Of the 67 newborns suspected to be G6 PD deficient based on FST(44 males,23 females),58/67(87%) were detected with gene *** kinds of mutations[c.95A>G,c.392G>T,c.493A>G,c.871G>A,C.1360C>T,c.1376G>T,and c.l388G>A]were identified by HRM ***:Routine newborn screening in Chaozhou,China with a relatively high prevalence of G6 PD deficiency is justified and meets the World Health Organization *** usage of molecular diagnosis can favor the detection of heterozygotes which can be a supplement to regular newborn screening and useful for premarital and prenatal diagnosis for G6 PD deficiency.

关键词： High resolution melting analysis for the neonatal screening of G6PD deficiency FST

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MET Is Required for the Maximal Action of 20-Hydroxyecdysone during Bombyx Metamorphosis

MET Is Required for the Maximal Action of 20-Hydroxyecdysone...

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广东省遗传学会第九届代表大会暨学术研讨会

作者：郭恩 Qianyu He Shumin Liu Ling Tian Zhentao Sheng Qin Peng Jingmin Guan Mingan Shi Kang Li Lawrence I.Gilbert Jian Wang 曹阳李胜 Key Laboratory of Insect Developmental and Evolutionary Biology Institute of Plant Physiology and EcologyShanghai Institutes for Biological SciencesChinese Academy of Sciences Laboratory of Insect Molecular Biology and Biotechnology Guangdong Provincial Key Laboratory of Agro-animal Genomics and Molecular BreedingCollege of Animal SciencesSouth China Agricultural University

Little is known about how the putative juvenile hormone(JH) receptor,the bHLH-PAS transcription factor MET,is involved in 20-hydroxyecdysone(20E;the molting hormone) *** we report that two MET proteins found in the silkworm,Bombyx mori,participate in 20 E signal *** is 20 E responsive and its expression peaks during molting and pupation,when the 20 E titer is *** found with results from RNAi knockdown of EcR-USP(the ecdysone receptor genes),RNAi knockdown of Met at the early wandering stage disrupts the 20E-triggered transcriptional cascade,preventing tissue remodeling(including autophagy,apoptosis and destruction of larval tissues and generation of adult structures) and causing lethality during the larval-pupal *** physically interacts with ***,MET,EcR-USP and the 20E-response element(EcRE) form a protein-DNA complex,implying that MET might modulate 20E-induced gene transcription by interacting with *** conclusion,the 20 E induction of MET is required for the maximal action of 20 E during Bombyx metamorphosis.

关键词： MET 20-Hydroxyecdysone Bombyx mori Metamorphosis

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Maternal serum disintegrin and metaIIoprotease protein-12 in early pregnancy as a potential marker of adverse pregnancy outcomes

Maternal serum disintegrin and metaIIoprotease protein-12 in...

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广东省遗传学会第九届代表大会暨学术研讨会

作者： Yang J Wu J Guo F Wang D Chen K Li J Du L Yin A Prenatal Diagnosis Centre Guangdong Women and Children Hospital

Background:The aim of this study was to determine whether the concentration of disintegrin and metalloprotease proteinl2（ADAM 12） in first trimester maternal serum can be used as a marker for first-trimester complete spontaneous abortions,missed abortions,ectopic pregnancies and hydatidiform ***:The maternal serum concentrations of ADAM12 were measured in the range of 5-9+6 weeks of gestation using an automated AutoDelfia immunoassay platform in 9 cases of complete spontaneous abortion,27 cases of missed abortions,56 cases of ectopic pregnancies,12 cases of hydatidiform moles,and 100 *** regression analysis was used to determine significant factors for predicting adverse pregnancyoutcomes in early *** performance was assessed using receiver operating characteristic ***:Two hundred and four women were enrolled in the *** the control group,the level of ADAM12 increased with gestational *** median ADAM12 levels in the spontaneous abortion（0.430 MoM）,ectopic pregnancy（0.460 MoM） and hydatidiform mole（0.037 MoM） groups were lower than that in the control group,while the median AD AM12 level in the missed abortion group（1.062 MoM） was not significant from the controls（1.002 MoM）.Logistic regression analysis demonstrated that the level of ADAM 12 in maternal serum facilitated the detection of ectopic pregnancies（OR=0.909;95%CI = 0.841- 0.982） and complete spontaneous abortion（OR = 0.863;95%CI= 0.787 ~ 0.946）.Conclusions:In complete spontaneous abortion and ectopic pregnancy,ADAM 12 maintained at low levels in early pregnancies,and there were significant differences compared to normal ***12 is a promising marker for the diagnosis of complete spontaneous abortion and ectopic pregnancy in symptomatic women,and under certain conditions,ADAM 12 can diagnose ectopic pregnancy and spontaneous abortion before an ultrasonographic detection of the conditions.

关键词： MoM Maternal serum disintegrin and metaIIoprotease protein-12 in early pregnancy as a potential marker of adverse pregnancy outcomes

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Mapping of the genetic determinant for grain size in rice using a recombinant inbred line(RIL) population generated from two elite indica parents

Mapping of the genetic determinant for grain size in rice us...

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广东省遗传学会第九届代表大会暨学术研讨会

作者：刘迪林 Meihua Kang 王丰 Wuge Liu Chongyun Fu Jinhua Li Manshan Zhu Xueqin Zeng Yilong Liao Zhenrong Liu Huijun Huang Rice Research Institute Guangdong Academy of Agricultural Sciences Guangdong Provincial Key Laboratory of New Technology in Rice Breeding

Grain size is a major component of rice grain yield and grain *** we conducted genetic analysis for grain size using an F recombinant inbred lines（RILs）population derived from the cross between two elite indica parents,the slender-grain line Taifeng B（TFB） with good grain quality and the high-yielding short-grain cultivar Tesanai 2（TSA2）.Strong positive association between hundred-grain weight and grain width was observed,but significant negative correlation was found between grain length and grain width.A total of 31 QTLs for grain weight,grain length,grain width,grain length/width ratio and grain thickness were identified and they explained a total phenotypic variance ranging from 51.4%to 73.8%.Of them,9 QTLs were reported for the first time and they are likely novel genetic ***,QTLs for grain size tend to form clusters at similar *** of them were detected on chromosome 3,5,and 7,locating at the regions of the previously reported loci GS3,qSW5IGW5 and GS7,*** allele variance of the above three loci between the parental lines was verified by using functional marker and genome *** major QTLs and QTL clusters discovered in this study are valuable for improvement of rice grain yield and quality through marker-assisted selection（MAS）.The repeatedly detected QTLs and newly identified loci provide foundation for further fine mapping and map-based cloning.

关键词： Rice(Oryza sativa L) grain size QTL mapping pleiotropic effect marker-assisted selection(MAS)

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Arabidopsis IQM1 is involved in stomatal innate immune

Arabidopsis IQM1 is involved in stomatal innate immune

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广东省遗传学会第九届代表大会暨学术研讨会

作者：周玉萍 Qiong-Hua Chen Xiao-Ling Huang Zhang-Ke Huang Zhong-Zhen Mo 田长恩 School of life Sciences Guangzhou University

Arabidopsis IQMs share sequence homology with a pea heavy metal-induced protein 6 and a *** expression patterns for each gene suggest that each IQM family member may play a different role in plant development and response to environmental *** functions of the IQM family members remain to be *** can bind with AtCaM5 in yeast two-hybrid assay via its *** CaM binding was Ca-independent in vitro,and was also observed in bimolecular fluorescence complementation analyses in onion epidermal *** was found to express strongly in guard cells and the cortex of *** T-DNA insertion mutants of IQMl(iqml) displayed a smaller stomatal aperture,a decreased water loss rate and a shorter primary ***,iqml did not change its stomatal aperture when treated with light,dark,ABA and chitin *** guard cell of iqml-1 also showed enhanced expression of genes involved in production and signaling of reactive oxygen species(ROS).Consistently,increased ROS level was observed in guard ***,243 and 28 genes were up- and down-regulated by more than two fold in the leaf of iqml-1,***,34 of 117 and 7 of 30 chitin-responsive transcriptional factor and ubiquitin ligase genes were *** 51defense-associated genes in iqml-1 were also *** with above,iqml-1 was found to be more resistant to Pst DC3000 than wild type when the pathogen is sprayed,but to show similar symptom to wild type when the pathogen is infiltrated,suggesting that IQMl is involved in stomatal innate *** further results showed that mutation of IQMl reduces sensitivity to J A which enhances stomatal aperture and consequently reduces resistance to Pst DC3000,indicating that IQMl enhance J A signal transduction and stomatal open and consequently reduce resistance to Pst DC3000.

关键词： AtIQM1 Stomatal movement Reactive oxygen species Calmodulin-binding protein JA signaling Arabidopsis thaliana Stomatal innate immune Pst DC3000

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Gloverins of the silkworm Bombyx mori:Structural and binding properties and activities

Gloverins of the silkworm Bombyx mori:Structural and binding...

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广东省遗传学会第九届代表大会暨学术研讨会

作者：易辉玉 Xiao-Juan Deng Wan-Ying Yang Cong-Zhao Zhou 曹阳余小强 Laboratory of Insect Molecular Biology and Biotechnology Guangdong Provincial Key Laboratory of Agro-animal Genomics and Molecular BreedingCollege of Animal ScienceSouth China Agricultural University Division of Cell Biology and Biophysics School of Biological SciencesUniversity of Missouri-Kansas City School of Life Sciences University of Science and Technology of China

Glovenns are basic,glycine-nch and heat-stable antibacterial proteins(w14-kDa) in lepidopteran insects with activity against Escherichia coli,Gram-positive bacteria,fungi and a *** gloveri gloverin adopts a random coil structure in aqueous solution but has a-helical structure in membrane-like environment,and it may interact with the lipid A moiety of lipopolysaccharide(LPS).Manduca sexta gloverin binds to the O-specific antigen and outer core carbohydrate of *** the silkworm Bombyx mori,there are four gloverins with slightly acidic to neutral isoelectric *** this study,we investigate structural and binding properties and activities of *** gloverins(BmGlvs),as well as correlations between structure,binding property and *** BmGlv\-4 were expressed in bacteria and *** dichroism(CD) spectra showed that all four BmGlvs mainly adopted random coli structure(>50%) in aqueous solution in regardless of pH,but contained a-helical structure in the presence of l,l,l,3,3,3-hexafluoro-2-propanol(HFIP),smooth and rough mutants(Ra,Re and Re) of LPS and lipid *** ELISA assay showed that BmGlvs at pH 5.0 bound to rough mutants of LPS and lipid A but not to smooth *** activity assay showed that positively charged BmGlvs(at pH 5.0) were active against *** mutant strains containing rough LPS but inactive against *** with smooth *** results suggest that binding to rough LPS is the prerequisite for the activity of BmGlvs against ***.

关键词： Gloverin Lipopolysaccharide Circular dichroism Antibacterial Random coil a-helix Bombyx mori

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