：意大利蝗(Calliptamus italicus L.)是新疆荒漠半荒漠草原的主要危害种类,前期研究表明其发生与新疆气候变暖显著相关,本研究进一步探讨气候变暖条件下意大利蝗适应的呼吸代谢机制.方法：应用多通道昆虫呼吸仪测定了15℃、20℃、25℃...
详细信息
：意大利蝗(Calliptamus italicus L.)是新疆荒漠半荒漠草原的主要危害种类,前期研究表明其发生与新疆气候变暖显著相关,本研究进一步探讨气候变暖条件下意大利蝗适应的呼吸代谢机制.方法：应用多通道昆虫呼吸仪测定了15℃、20℃、25℃、30℃ 和35℃不同温度条件下意大利蝗的呼吸率、代谢率、CO2释放率、Q10及呼吸商并分析其变化特征.结果：15℃时意大利蝗的呼吸率、代谢率、CO2释放率均显著低于其它温度(P＜0.01),35℃时三项指标值显著高于其它温度(P＜0.01),表明低于15℃和高于35℃的温度条件都对意大利蝗的呼吸代谢产生明显影响;在20℃～30℃之间,意大利蝗的呼吸率、代谢率及CO2释放率变化幅度小且平稳,差异均不显著(P＞0.01),表明该温度范围是意大利蝗生长发育的适宜温度条件.不同温度下意大利蝗呼吸率的Q10值显示,20℃～30℃温度范围内的 Q10值最小,为1.03,15℃～25℃的Q10值为1.43,25℃～35℃的Q10值最大,为2.42,说明意大利蝗的呼吸代谢活动对温度变化敏感,并表明20℃～30℃是意大利蝗生长发育的适宜范围.各温度条件下意大利蝗呼吸商的差异不显著(P＞0.01),平均为0.945,并判断意大利蝗呼吸代谢消耗的底物主要为糖类物质.结论：本研究得出20℃～30℃是意大利蝗生长发育的适宜范围,预示着在气候持续变暖背景下,意大利蝗仍将是新疆草原最重要的生物灾害之一.
目的：构建骆驼蓬脂转移蛋白(lipid transfer protein from Peganum harmala,PhLTP)基因真核表达质粒,并探讨其对黑色素瘤B16细胞在体内外的抗肿瘤作用. 方法：将PhLTP基因亚克隆至pcDNA3.1上,获得重组质粒pcDNA3.1-PhLTP;用脂质体...
详细信息
目的：构建骆驼蓬脂转移蛋白(lipid transfer protein from Peganum harmala,PhLTP)基因真核表达质粒,并探讨其对黑色素瘤B16细胞在体内外的抗肿瘤作用.
方法：将PhLTP基因亚克隆至pcDNA3.1上,获得重组质粒pcDNA3.1-PhLTP;用脂质体转染法将重组质粒及空载体外转染B16细胞,MTT检测其对B16细胞生长的影响.建立B16荷瘤小鼠模型,设重组质粒(pcDNA3.1-PhLTP)、空载(pcDNA3.1)、生理盐水和阳性药物(CTX)组,分别处理小鼠后测量各组肿瘤体积并称瘤重,计算抑瘤率.光镜观察鼠脾、肝等组织变化;免疫组织化学法检测各瘤体中PhLTP、血管内皮生长因子(VEGF)及碱性成纤维细胞生长因子(bFGF)的表达.
结果：pcDNA3.1-PhLTP转染B16细胞72 h后,细胞增殖能力明显受到抑制(P ＜ 0.01).注射pcDNA3.1-PhLTP组的小鼠肿瘤生长速度明显减慢,肿瘤体积小于空载和生理盐水组(P ＜ 0.05).显微镜下可见重组质粒组肿瘤细胞有不同程度的点、片状坏死,而肝、肺等无明显病理损伤.重组质粒组肿瘤组织中有PhLTP蛋白的表达,且VEGF和bFGF的阳性表达指数都低于空载和生理盐水组(P ＜ 0.01).
结论：成功构建了重组表达质粒pcDNA3.1-PhLTP,体内外实验结果显示其能有效地抑制B16细胞的生长,预示了该重组质粒在治疗黑色素瘤中的潜在应用价值.
Cold acclimation usually alters gene expression in *** order to get the further information about the up-regulated Attacin gene (MpAttacin) obtained from the transcriptomic data generated at 4℃ from the desert beetle...
详细信息
Cold acclimation usually alters gene expression in *** order to get the further information about the up-regulated Attacin gene (MpAttacin) obtained from the transcriptomic data generated at 4℃ from the desert beetle Microdera punctipennis,and to analyze the responsive expression of these genes induced by low temperature,MpAttacin were characterized by bioinformatic *** analysis showed that the encoded product of these genes shares 30%-57% identity at the amino acid level with Attacins from other insects of Lepidoptera,Diptera,and *** phylogenetic tree generated by Neighbor-Joining method indicated that MpAttacins and Attacin proteins from other coleopterans are descended from a common ancestor,and they belong to Attacin C ***-time quantitative PCR was performed to detect the mRNA level of MpAttacins at low *** of real-time quantitative PCR showed that the expression of MpAttacins presented a stress-response tendency when stressed at both 4℃and -4℃,decreasing first and then ***,there were differences in their responsive time and strength between these two *** Mpattacin transcripts were detected at significant level in hindgut and Malpighian tube,These results suggest that in addition to the typical function as an anti-microbial peptide,attacin may also be involved in the cold adaptation of insects.
暂无评论