It is well established that both ischemia-preconditioned astrocytes and neurons can protect neurons against ischemia-induced *** compelling evidence has indicated the existence of bi-directional communications between...
It is well established that both ischemia-preconditioned astrocytes and neurons can protect neurons against ischemia-induced *** compelling evidence has indicated the existence of bi-directional communications between neurons and astrocytes under physiological conditions, we speculated that preconditioned neurons, like astrocytes, could protect astrocytes from ischemia-induced injury as *** the present study, we investigated the effects of preconditioned neurons on astrocytes in ischemia *** results demonstrated that ischemia-preconditioned neurons significantly enhanced the viability of astrocytes undergoing ischemia, most likely via anti-apoptosis, which was mediated by the molecular changes of reduced level of active caspase-3, increased expression of anti-apoptotic factor Bcl-2 and Bcl-xL as well as phosphorylation of pro-apoptotic factor Bad in ischemic *** also found that the preconditioning of neurons could increase the expression and release of erythropoietin (EPO), and addition of anti-EPO antibody blocked the observed protective effect of ischemia-preconditioned *** analyses revealed that the activation of phosphatidylinositol 3-kinase (PI3K), extracellular signal-regulated kinase (ERK) and signal transducer and activator of transcription 5 (STAT5) signaling pathways induced by EPO contributed to the anti-apoptotic action of ischemia-preconditioned neurons on *** our in vivo model of forebrain ischemia, it was found that prior destruction of cortical neurons by kainic acid (KA) injection resulted in a significant appearance of apoptosis in astrocytes, which was accompanied by a reduction in up-regulated EPO content induced by IP *** results imply that ischemia-preconditioned neurons can protect astrocytes against ischemia-induced apoptosis and EPO plays a critical role in this process.
Fas and its ligand (FasL) play important roles in the apoptosis and *** in Fas (-670A>G,-1377G>A,) and FasL (-844C>T) implicated in cancer risk had been widely ***, the conclusions were always ***'s urgen...
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Fas and its ligand (FasL) play important roles in the apoptosis and *** in Fas (-670A>G,-1377G>A,) and FasL (-844C>T) implicated in cancer risk had been widely ***, the conclusions were always ***'s urgent to carry out a meta-analysis to achieve a more precise estimation of the relationship between polymorphisms in Fas or FasL and cancer *** studies were selected by PubMed and Web of Science, and 52 eligible literatures were involved in this *** Fas-670A>G polymorphism, no significant association was observed in the overall *** for Fas-1377G>A polymorphism, results revealed AA genotype was associated with elevated cancer risk in the overall *** stratifications showed increased risks in breast, gastric and esophageal cancer, Asian population, population-based and hospital-based controls and studies carried by the method of *** FasL-844C>T, a significantly elevated cancer risk was associated with CC genotype in the overall *** stratifications showed increased risks in gastric, esophageal and ovarian cancer, Asian population, population-based and hospital-based controls and studies carried by the method of *** meta-analysis indicated the A allele ofFas-1377G>A and C allele of FasL-844C>T were risk factors for most of cancers.
Objective: In this study, we aimed to reduce the expression of CD147 by RNA silencing, investigating the effect of CD 147 silencing to the human colorectal cancer cells proliferation, invasion and drug ***: We constru...
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Objective: In this study, we aimed to reduce the expression of CD147 by RNA silencing, investigating the effect of CD 147 silencing to the human colorectal cancer cells proliferation, invasion and drug ***: We constructed a novel short hairpin RNA (shRNA) expression vector *** CD147 shRNA plasmid was transferred to human colorectal cancer cell line *** expression of CD147, MCT1 and MCT4 were monitored by quantitative real-time RT-PCR and Western blotting, *** gelatin zymography assay determined the MMP-2 and MMP-9 *** intracellular lactaten concentration was determined by the lactic acid assay ***-8 assay was applied to determine the HT29 cells proliferation and the chemosensitivity to *** assay was used to determine the invasion of HT29 *** addition, we selected the 4-6-week-old female nude mice to establish subcutaneous tumor model of colorectal cancer, and applie-d the immunohistochemical analysis to detect CD147 expression in ***: The results showed that the CD147 and MCT1 expression were significantly reduced at both mRNA and protein levels, as well as the MMP-2 and MMP-9 *** proliferation and invasion ability were also decreased in HT29 cells, but increased chemosensitivity to *** vivo, the CD 147 expression was also significantly decreased, and reduced the tumor growth after CD147 gene ***: The results demonstrated that silencing of CD147 expression inhibited the proliferation and invasion of colorectal cancer cells, but increased its chemosensitivity to cisplatin and decreased the secretion of MMPs in colorectal cancer cells, suggesting CD 147 silencing might be an adjuvant gene therapy strategy to chemotherapy.
To study the inhibitory effects of broccoli in colon cancer cells line SW620 and its possible ***620 cells were treated with broccoli extract in different *** order to further explore the anti-tumor mechanisms of broc...
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To study the inhibitory effects of broccoli in colon cancer cells line SW620 and its possible ***620 cells were treated with broccoli extract in different *** order to further explore the anti-tumor mechanisms of broccoli, with different concentration broccoli crude extract processing colon cancer cell line SW620, using MTT and drawing growth curve, this experimental investigates the SW620 cell proliferation *** transcription and expression level change of SW620 of apoptosis related gene p53 and caspase-3 were detected by q-PCR, analysis the influence mechanisms of broccoli crude extract on SW620 cell proliferation and *** results showed that different concentrations of broccoli crude extract has obvious inhibition effect on SW620 cell proliferation which increases obviously with the increasing concentration, and the highest inhibition rate reaches as high as 90.1%.By MTT method IC50 of broccoli crude extract has calculated as 0.1 mg/*** processing by the broccoli crude extract on IC50, the following changes of SW620 cell has occurred: the growth of cells is obviously restrained, cell falls off, deformation and adherent ability drops, appearing the phenomenon of *** genome DNA banding appears obviously tailing phenomenon, the transcriptional level of caspase-3 detected by fq-PCR compared with the control group has increased more than three times, moreover, the same level of p53 has increased dozens of *** research results show that the broccoli crude extract can inhibit the growth of colon cancer cell line SW620, and its inhibition mechanism may be show that the active material induced the up-regulated expression of p53 and caspase-3 those two important apoptosis regulation gene in apoptosis pathway, leading the cell apoptosis.
Fas and Fas ligand system is one of the key components in the apoptotic *** -1377G>A,-670A>G and FasL-844T>C polymorphisms affecting the genes expression are widely investigated in breast ***, the migration a...
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Fas and Fas ligand system is one of the key components in the apoptotic *** -1377G>A,-670A>G and FasL-844T>C polymorphisms affecting the genes expression are widely investigated in breast ***, the migration and invasion inhibitory protein (MIIP) is paid close attention in recent *** reveal the effect ofFas, FasL and MIIP (-167A>G) polymorphisms to the occurrence of breast cancer risk, herein 439 breast cancer patients and 439 controls were enrolled in this *** genotypes were detected by MassARRAY, and the estrogen receptor, progesterone receptor, CerbB-2 were determined by *** the 439 patients, 22 breast cancer and adjacent normal tissues were collected to detect the mRNA level of Fas by real-time PCR, and soluble Fas and FasL concentrations of 180 patients were detected by enzyme-linked immuno sorbent *** results showed Fas-1377GA, AA and GA/AA genotypes were associated with decreased breast cancer risk, the same as Fas-670AG, GG and AG/GG *** FasL-844T>C polymorphism, TC and CT/TT genotype were associated with decreased breast cancer ***, haplotype analysis indicated Fas-1377G/-670A was associated with increased breast cancer risk, while-1377A/-670A with an opposite *** gene-gene interaction analysis of Fas and FasL polymorphisms revealed Fas-1377GA/AA (Fas -670AG/GG) and FasL-844CC or TC/TT genotypes associated with decreased breast cancer ***, it was found that-1377GG genotype was associated with higher sFas concentration than other genotypes, the same as-670AA *** for MIIP-167A>G polymorphism, AG and AG/GG genotypes were associated with decreased breast cancer *** findings indicate that Fas, FasL and MIIP polymorphisms can affect breast cancer risk, and Fas polymorphisms effect breast cancer risk by regulating the sFas *** to some limitations of this study, the conclusion deserves further elucidation in larger and multi-ethnic pop
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