Introduction:The objective of this study was to identify biomarkers of sepsis-induced disseminated intravascular coagulation(DIC) among platelet-derived factors using biotin label-based custom protein microarray techn...
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Introduction:The objective of this study was to identify biomarkers of sepsis-induced disseminated intravascular coagulation(DIC) among platelet-derived factors using biotin label-based custom protein microarray technology in a mouse cecal ligation and puncture (CLP) ***:KM mice were randomized into sham-operated and CLP groups. Blood samples were obtained immediately and at 1 h,2 h,6 h,12 h,24 h,48 h and 72 h after establishment of the CLP for platelet count,coagulation assay and blood *** and mesentery tissues were examined histologically at all corresponding time points,looking for microthrombus *** protein microarray analysis was performed to detect platelet-derived ***:The survival rate 72 h post-CLP was 15%,but there was no mortality among the sham-operated *** with the sham group,the platelet count (n = 5,p<0.05),fibrinogen concentration(n = 5,p<0.05) and alanine aminotransferase level of the CLP group began to decrease significantly at 6 h *** prolongation of prothrombin time(n = 5,p<0.05) and activated partial thromboplastin time(n = 5,p<0.05) and elevation of D-dimer(n=5,p<0.05) occurred after 6 h *** histology, microthrombus formation in lung and mesentery tissue was observed in the CLP groups 6 h post-CLP and had become significant and extensive 12 h post-CLP(n=5,p<0.05).On protein microarray analysis,thrombospondin(TSP),tissue inhibitor of metalloproteinase 1 (TIMP-1) and thymus chemokine-1(TCK-1) all increased during the first 2 h post-CLP,then remained at a higher level than in the sham group for 72 h post-CLP(n=5,p<0.05). Conclusions:TSP,TIMP-1 and TCK-1 are elevated in the early stage of sepsis-induced DIC in a mouse CLP model and may be considered early markers for sepsis-induced DIC.
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