Gene transfer vector is one of the key technologies of gene *** carriers generally can be divided into two major categories of virus and non-virus,the former is subjected to security *** latter much favored in medical...
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Gene transfer vector is one of the key technologies of gene *** carriers generally can be divided into two major categories of virus and non-virus,the former is subjected to security *** latter much favored in medical science and also is currently the main object of pharmaceutical research because of its safety,vector design diversity and low *** non-virus carrier,polycation gene carrier due to the high rate of transfection and become a main research ***(PAMAM) is one of the important member in the family of cationic polymer carrier,PAMAM dentrimers are attracted highly attention recently due to their special properties in the drug and gene delivery systems,especially the high generation PAMAM as the gene vector shows strength transfection ability compared with the other non-viral carrier *** main drawback for the materials is presenting serious cell *** order to overcome this problem,one new gene delivery system has been designed by our research *** is that PAMAMs are covalently conjugated onto α-cyclodextrin(α-CD) via amide bonds to obtain the starburst-like cationic polymers(CD-PG2).The chemical structure and composition of CD-PG2 was characterized by *** physicochemical and biological properties of CD-PG2/pDNApolyplex were evaluated by agarose gel retardation,stability test against DNase I,MTT assay,DLS measurement,CLSM observation,LDH leakage test,and in vitro cell *** test results tell us that the CD-PG2 could efficiently condense pDNA into nano-scale particles with a narrow size distribution,and protest from DNase I ***-PG2 shows excellent gene transfection efficiency without serum interference as well as relatively low cytotoxicity,compared with free PET-25 *** commercial product Lipofectamine 2000(one good gene vector).The cell uptake route analysis shows cellular uptake CD-PG2/pDNApolyplex is mainly through Clathrin-mediated endocytosis,CME and Caveolae-medi
The genus Pholidota(Orchidaceae) belongs to the tribe Coelogyneae,and comprises ca.30 species with a distribution from tropical Asia to tropical *** them,ca.14 species occur in *** Cantonensis Rolfe.,named ’Xi-Ye-Shi...
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The genus Pholidota(Orchidaceae) belongs to the tribe Coelogyneae,and comprises ca.30 species with a distribution from tropical Asia to tropical *** them,ca.14 species occur in *** Cantonensis Rolfe.,named ’Xi-Ye-Shi-Xian-Tao’ in Chinese,is a Chinese folk herbal medicine,with a long history of safe use for treating sore throat,tonsillitis,cough phlegm asthma,pneumonia,acute soft tissue trauma and *** phytochemical study on *** showed the existence of stibenoids,cycloartane triterpenoid and *** on the previous study the 80%ethanol extract of the whole plant *** was separated by chromatography on silica gel,Sephadex LH-20,MCI gel and afford22 compounds,the structures of compounds were elucidated on the basis of the chemical evidence and H-NMR,C-NMR,2D-NMR and MS *** new constituents were isolated by the column chromatography over silica gel,MCI gel,RP-18 silica gel,Sephadex LH-20 and pre-HPLC from the EtOH extract of the air-dried whole plant of Pholidota cantonensis Rolfe.,together with 18 known *** structures of the new compounds were established as l-hydroxy-2,7-dimethoxy-9,10-dihydrophenanthrene-[4,5-bcd]furan(1),5-hydroxy-2,7-dimethoxy-9,10-dihydro-l,4-Phenanthrenedione(2),ent-labd-13(E)-ene-3α,8β,15-tri-3-O-β-D-glucopyranoside(3),5α,10β-ent-cleroda-3,13(E)-diene-15,18-diol-15-0-β-D-glucopyrannoside-18-O-β-Dglucopyrannoside(4) by chemical and spectroscopic methods,including ID and 2D NMR.
Ethnopharmacological relevance:compound Muniziqi granule（MNZQ）,a traditional Uighur medical preparation,is made up of 13 species of medicinal *** is traditionally used for regulating the body's immunity,eliminat...
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Ethnopharmacological relevance:compound Muniziqi granule（MNZQ）,a traditional Uighur medical preparation,is made up of 13 species of medicinal *** is traditionally used for regulating the body's immunity,eliminating inflammation and analgesic,detoxification,and anti-tumor in *** aim of present study is to scientifically evaluate the anti-intlammatory and analgesic activities of MNZQ and to support its clinic use with scientific evidence,and lay a foundation for its further *** analgesic activity was evaluated by hot plate test and acetic acid induced abdominal writhing *** acute inflammatory was evaluated by models of xylene-induced ear oedema and carrageenan-induced paw oedema,and the chronic inflammatory was evaluated by cotton pellets-induced granuloma *** the two tests of analgesic activities,MNZQ produced significant dose-dependent manner increased latency in the hot-plate test,and the percentage of inhibition suggested that it has centrally analgesic *** in acetic acid-induced abdominal writhing in mice,MNZQ showed strongly inhibition on the acetic acid induced writhing response for 25.22%（P < 0.01）,44.60%（P < 0.001） and 49.41%（P < 0.001） at dosages of 0.8,2.4 and 7.2 g·*** results suggested that MNZQ also has peripheral analgesic *** for anti-inflammatory activities,MNZQ showed significant dose-dependent manner inhibition on xylene-induced oedema and the percentage inhibition was 22.24%（P < 0.01） at the highest dosage of 7.2 g·*** the dosages of 1.62 and 4.86 g·kg,MNZQ could significantly reduce carrageenan induced rat hind paw edema by82.43%and 84.32%（P < 0.001） after 1h injecting carrageenan and kept to 5 *** also exerted a significant anti-inflammatory effect against cotton pellet induced granuloma *** the dosages of 1.62 and 4.86 g·kgproduced 17.07%,17.60%inhibition of granuloma formation,whereas diclofenac produced 33.12%inhibition in granuloma *** results demonstrat
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