Preparedness for a looming influenza pandemic caused by highly pathogenic avian influenza (HPAI) A subtype H5N1 has become a global priority. H5N1 disease outbreaks in migratory waterfowl in western China, the continu...
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Preparedness for a looming influenza pandemic caused by highly pathogenic avian influenza (HPAI) A subtype H5N1 has become a global priority. H5N1 disease outbreaks in migratory waterfowl in western China, the continuing geographic spread of the virus in Eurasia, and the emergence of human infection in Indonesia have heightened pandemic concern. It remains unknown from where the pandemic strain may emerge;current attention is directed at Vietnam, Thailand, Cambodia, and
AIM: It was the infectation of hepatovirus that results in hepatitis and hepatocirrhosis mainly. The primary objective of this study is to establish an animal model of hepatic fibrosis by the duck hepatitis B virus (D...
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AIM: It was the infectation of hepatovirus that results in hepatitis and hepatocirrhosis mainly. The primary objective of this study is to establish an animal model of hepatic fibrosis by the duck hepatitis B virus (DHBV) . METHODS: Each duckling born within 24 hours was injected with 0.1ml DHBV positive serum into vena cruralis once a week. From the 10th week,the dosage was increased to 0.2ml each time. Duck was killed after 16 *** indexes of hepatic fibrosis were assayed and the hepaticr histopathologyic changes and hepatocellular ultrastructure were observed. RESULTS: On hepaticr histopathological evaluation, the hepatic tissue of DHBV-induced model groups have hepatocellular oedema, fatty degeneration, inflammation and collagen deposition, which had no significant difference with the CCl4-induced model groups (P>0.05) . The level of serum type Ⅲ procollagen (PCⅢ) and serumal hyaluronic acid (HA) and hydroxyproline (Hyp) of hepatic tissue were increased significantly (P<0.01, P0.05, P0.01) . CONCLUSION The results showed that DHBV-induced duck model of hepatitis fibrosis is established successfully.
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