Although clinical application of mild hypothermia(32℃)for patients with brain injury and cardiac arrest has been carried out with promising results,the molecular mechanisms underlying the protective effects of mild h...
详细信息
Although clinical application of mild hypothermia(32℃)for patients with brain injury and cardiac arrest has been carried out with promising results,the molecular mechanisms underlying the protective effects of mild hypothermia are not fully *** deal with the question,we first examined the effects of mild hypothermia on H9C2 rat ventricular myoblasts underwent apoptosis in conditions of simulated ischemia,represented by serum withdrawal plus hypoxia.H9C2 cell apoptosis is evidenced by an increased apoptotic cells with Annexin-V positive and propidium iodide negative by flow cytometry,DNA fragmentation,and activation of caspase *** this model of simulated ischemia,cardiomyoblasts apoptosis was associated with a down-regulation of small molecular weight proteins including heat shock protein(HSP)20,HSP27,andαβ-crystallin and a reduction of cell *** hypothermia significantly reduced the simulated ischemia-induced apoptosis,down-regulation of small molecular weight proteins, and reduction of cell ***,our findings have further shown that mild hypothermia causes amelioration of hypotension by enhancing stroke volume,heart beat,and total peripheral resistance in the rat during heatstroke. A comparative analysis using two-dimensional in-gel electrophoresis of cardiac protein patterns is performed in rat controls,untreated heatstroke rats,and mild hypothermia-treated heatstroke *** the onset of heatstroke, animals display hypotension and down-regulation of cardiac small molecular weight proteins,which can be reversed by mild *** data indicate that mild hypothermia may act via normalization of small molecular weight protein expressionand cardiac function to exert its therapeutic effect in ischemic hearts.
暂无评论