Intestinal trefoil factor(ITF,also named as trefoil factor 3,TFF3)is a member of the TFF-domain peptide family,which plays an essential role in regulating cell survival,cell migration and maintains mucosal epithelial ...
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Intestinal trefoil factor(ITF,also named as trefoil factor 3,TFF3)is a member of the TFF-domain peptide family,which plays an essential role in regulating cell survival,cell migration and maintains mucosal epithelial integrity in gastrointestinal ***,the underlying mechanisms and associated molecules remain *** aim of this study was to explore the protective effects of ITF on gastric mucosal epithelium injury and its possible molecular *** the present study,we show that ITF could promote proliferation and migration of GES-1 cells via a mechanism that involves the PI3K/Akt signaling *** blot results indicated that ITF induced a dose-and time-dependent increase in Akt signaling *** also plays an essential role in restitution of GES-1 cells damage induced by LPS(Lipopolysaccharide).LPS induced GES-1 cells apoptosis,decreased cell viability significantly(P<0.01)and led epithelial tight junction damage which is attenuated via ITF *** protective effect of ITF on the integrity of GES-1 was abrogated by inhibition of the PI3K/Akt *** together,our results demonstrate that ITF promotes proliferation and migration of gastric mucosal epithelial cells and preserves gastric mucosal epithelial integrity after damage is mediated by activation of PI3K/Akt signaling *** study suggested that PI3K/Akt pathway could act as a key intracellular pathway in the gastric mucosal epithelium that may serve as a therapeutic target to preserve epithelial integrity during injury.
We sought to explore the treatment effects and the repair mechanisms of bone marrow derived mesenchymal stem cells(MSCs)during HCl-induced acute lung injury(ALI).MSCs were delivered through the tail veins of rats 24h ...
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We sought to explore the treatment effects and the repair mechanisms of bone marrow derived mesenchymal stem cells(MSCs)during HCl-induced acute lung injury(ALI).MSCs were delivered through the tail veins of rats 24h after intraperitoneally injection of *** results showed that MSCs did not ameliorate the histopathologic changes of ALI and pulmonary *** found that the activated Wnt/β-catenin signaling may regulate the differentiation of MSCs and is associated with lung fibroblasts activation,pulmonary fibrosis and tissue repair process in ALI *** and histology analysis indicated that activated canonical Wnt/β-catenin signaling induced most MSCs to differentiate into myofibroblasts or fibroblasts in ***,inhibition of Wnt/β-catenin signaling by Dickkopf-1(DKK1)promotes epithelial differentiation of MSCs induced by native alveolar epithelial cells which are beneficial to repair the injured lung *** of Wnt/β-catenin signaling after MSCs transplantation ameliorated pulmonary fibrosis and improved pulmonary function which attenuated the lung *** vitro study,activation of the Wnt/β-catenin signaling stimulated MSCs to express myofibroblasts markers,which was attenuated by ***,Wnt3αactivated Wnt/β-catenin signaling in lung fibroblasts to enhance the expression of collagen I,vimentin andα-smooth muscle actin,but DKK1 attenuated these proteins *** findings demonstrated that canonical Wnt/β-catenin signaling plays a key role in regulating differentiation of MSCs in vivo or in vitro and the pathogenesis of fibrotic *** study suggested that inhibition of abnormal activated Wnt/β-catenin signaling would promote MSCs epithelial differentiation to repair lung injury and reduce pulmonary fibrosis.
Objective:To investigate the effects of molecular hydrogen(H2)and suberoylanilide hydroxamic acid(SAHA),a histone deacetylase inhibitor,on paraquat(PQ)-stimulated production of reactive oxygen species(ROS)and TNF-αin...
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Objective:To investigate the effects of molecular hydrogen(H2)and suberoylanilide hydroxamic acid(SAHA),a histone deacetylase inhibitor,on paraquat(PQ)-stimulated production of reactive oxygen species(ROS)and TNF-αin ***:To determine the optimal concentration of PQ for use in experiment to study the effects of H2 and SAHA on PQ-stimulated RAW264.7 macrophages,serum-starved cells were treated with PQ at 0,0.001,0.01,0.1,1 and 10 *** 1,2 and 8 h,cell viability was examined using the trypan blue exclusion method,intracellular ROS levels were evaluated using the fluorescent DCFH-DA probe and TNF-αlevels in the culture media were determined by *** on the results obtained,mouse RAW267.4 macrophages were then treated with PQ in combination with SAHA and/or H2 for 8 h Results:A significant stimulative but nontoxic effect of PQ on RAW267.4 macrophages was observed at 0.1 mM;this concentration was used in the subsequent studies.H2 and H2 combined with SAHA caused a greater decrease in PQ-induced ROS production than SAHA alone,especially at 2 h and 8 *** 1 h and 2 h,treatment involving H2 caused a greater decrease in PQ-induced production of TNF-αcompared with treatment without ***,at 8 h,treatment involving SAHA induced more pronounced effects on TNF-αthan treatment without ***:H2 decreases PQ-induced ROS production and attenuates early PQ-induced TNF-αproduction while SAHA reduces PQ-induced TNF-αproduction at the late phase in *** effects are enhanced by H2 combined with SAHA.
Objective:This study aims to assess the prognostic significance of urine paraquat（PQ）concentrations on admission at emergency room in patients with acute PQ *** and Method:From January 2009 to December 2013,a total ...
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Objective:This study aims to assess the prognostic significance of urine paraquat（PQ）concentrations on admission at emergency room in patients with acute PQ *** and Method:From January 2009 to December 2013,a total of 306 patients with acute PQ poisoning were admitted to the Emergency Intensive Care Unit（EICU）at the First Affiliated Hospital of China Medical *** relationships between urine PQ concentrations and prognosis of patients with acute PQ poisoning were *** was tested using the sodium dithionite reaction and plasma PQ levels were measured at ED using high-performance liquid chromatography since November ***:The median dose of ingested PQ among patients with acute PQ poisoning was 45 ml（range:10-280 ml,IQR:60 ml）.The mean time interval between PQ ingestion and presentation for medical care was 2.9±0.9 *** overall mortality rate was 70.9%（217/306）over a 28-day follow-up period,38 patients died within 7 days following PQ poisoning,and 179 patients died between 7 and 28 *** was a significant difference in admission urine PQ concentrations between non-survivors and survivors（P=0.022）.Receiver operating characteristic（ROC）curve analysis reveals that the area under the ROC curve（AUC）of admission urine PQ concentrations for predicting mortality was 0.854 with a cut-off value of 34.5μg/ml（sensitivity,84.8%;specificity,42.7%;Youden index=0.275）.The cumulative survival rate differed significantly between patients with urine PQ concentration<34.5μg/ml and≧34.5μg/ml（46.9%vs.2.7%,x=18.024,P=0.000,OR=8.96,95%CI=5.19-13.71）.The results indicated that there was a positive correlation between the urine and the plasma PQ concentration（r=0.532,P=0.002）.The dose of PQ ingested,arterial lactate and urine concentration of PQ were independent risk factors predicting 28-day *** time interval between ingestion and hemoperfusion,arterial lactate and urine concentration of PQ were independent risk factors predicting AKI a
Background:Thousands of paraquat（PQ）-poisoned patients continue to die,particularly in developing countries,plasma perfusion has recently been incorporated as a method of clinical *** purpose of this study was to es...
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Background:Thousands of paraquat（PQ）-poisoned patients continue to die,particularly in developing countries,plasma perfusion has recently been incorporated as a method of clinical *** purpose of this study was to estimate the paraquat clearance of plasma perfusion,and observe the effect of plasma perfusion on paraquat concentration in the blood of patients with acute paraquat ***:21 PQ-poisoned patients admitted to our poisoning center within 24 hours after the ingestion were prospectively *** plasma perfusion（flow rate 180 ml/min）was performed,with plasma/blood separation at 40 ml/min and routine cartridges change every 3 *** PQ（UPQ）,urine flow rate（UFR）,and plasma PQ（PPQ）concentration level at inlet/outlet of the cartridge were obtained right before,and 1.5 hours after the start of each perfusion session for calculation of renal and plasma PQ ***:In all 8 rounds（108 sessions）of plasma perfusion on the 21 patients,PQ clearance rate（ml/min）by plasma perfusion was found always higher than the renal value:[1st（21 cases）11.14±6.13 vs.6.53±1.46;2nd（21 cases）18.36±11.32 vs.6.23±1.51;3rd（21 cases）16.13±10.05vs.4.01±0.93;4th（17 cases）12.86（6.72,17.47）vs.2.42（0.65,4.20）;5th（11 cases）14.12（10.48,35.20）vs.1.77（0.63,2.91）;6th（7 cases）16.47（11.82;20.69）vs.1.70（0.23,3.18）;7th（5 cases）13.33（9.71,18.75）vs.1.10（0.14,2.99）;8th（5 cases）11.27（9.21,16.02）vs.1.10（0.09,2.79）,P<0.05 or P<0.01].The survivors（10 cases）showed a higher PPQ reduction rate(mg×L×h)than the non-survivors（11 cases,0.57±0.03 vs.0.47±0.06,P<0.05）.Conclusion:Our data show that plasma perfusion therapies help in the clearance of PQ and may prove a promising therapeutic tool in patients with acute PQ intoxication.
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